General Methodology and Apparatus:
P.-+. Albertsson and F. Tjerneld, Phase Diagrams.
D.E. Brooks and R. Norris-Jones, Preparation and Analysis of Two-Phase Systems.
G. Johansson, Partitioning Procedures and Techniques: Small Molecules and Macromolecules.
H. Walter and C. Larsson, Partitioning Procedures and Techniques: Cells, Organelles, and Membranes.
G. Johansson, Uses of Poly(ethylene Glycol) with Charged or Hydrophobic Groups.
G. Johansson and M. Joelsson, Systems Containing Organic Solvents.
P.-+. Albertsson, Chiral Resolution.
H.E. Kerlund and P.-+. Albertsson, Thin-Layer Countercurrent Distribution and Centrifugal Countercurrent Distribution Apparatus.
W. Maller, Columns Using Aqueous Two-Phase Systems.
P.-O. Larsson, Magnetically Enhanced Phase Separation.
Partitioning and Affinity Partitioning of Macromolecules: Proteins:
G. Kopperschlager, Affinity Extraction with Dye Ligands.
M. Joelsson and F. Tjerneld, Purification of Lactate Dehydrogenase from Pig Muscle by Affinity Partitioning.
G. Kopperschlager, Phosphofructokinase from Baker's Yeast.
G. Birkenmeier, Partitioning of Blood Proteins Using Immobilized Dyes.
B.H. Chung, D. Bailey, and F.H. Arnold, Metal Affinity Partitioning.
A. Blennow, Starch Synthase from Potato Tubers.
J.S. Brusca and J.D. Radolf, Isolation of Integral Membrane Proteins by Phase Partitioning with Triton X-114.
W. Maller, Separation of Proteins and Nucleic Acids.
G.E. Francis and D. Fisher, Purification of Nucleic Acid-Protein Comple
This volume of Methods in Enzymology represents the sole collection of separation methods based on aqueous two-phase systems. It includes procedures for the isolation of proteins, especially enzymes, nucleic acids, cell membranes, and organelles, as well as for the separation and study of cells.
@introbul:Key Features @bul:* Use of affinity partitioning for enzyme purification
- Separation and study of cells
- Isolation of plasma membranes
- Large scale biotechnical use
Biochemists, biophysicists, molecular biologists, cell biologists, physical chemists, microbiologists, biotechnologists, plant scientists.
- No. of pages:
- © Academic Press 1994
- 18th April 1994
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
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Aqueous Phase Systems, Washington, D.C., U.S.A.
University of Lund, Sweden
California Institute of Technology, Division of Biology, Pasadena, U.S.A.
The Salk Institute, La Jolla, CA, USA