Aqueous Two-Phase Systems, Volume 228

1st Edition

Serial Volume Editors: Harry Walter Göte Johansson
Editor-in-Chiefs: John Abelson Melvin Simon
Hardcover ISBN: 9780121821296
eBook ISBN: 9780080883410
Imprint: Academic Press
Published Date: 18th April 1994
Page Count: 725


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Table of Contents

General Methodology and Apparatus:

P.-+. Albertsson and F. Tjerneld, Phase Diagrams.

D.E. Brooks and R. Norris-Jones, Preparation and Analysis of Two-Phase Systems.

G. Johansson, Partitioning Procedures and Techniques: Small Molecules and Macromolecules.

H. Walter and C. Larsson, Partitioning Procedures and Techniques: Cells, Organelles, and Membranes.

G. Johansson, Uses of Poly(ethylene Glycol) with Charged or Hydrophobic Groups.

G. Johansson and M. Joelsson, Systems Containing Organic Solvents.

P.-+. Albertsson, Chiral Resolution.

H.E. Kerlund and P.-+. Albertsson, Thin-Layer Countercurrent Distribution and Centrifugal Countercurrent Distribution Apparatus.

W. Maller, Columns Using Aqueous Two-Phase Systems.

P.-O. Larsson, Magnetically Enhanced Phase Separation.

Partitioning and Affinity Partitioning of Macromolecules: Proteins:

G. Kopperschlager, Affinity Extraction with Dye Ligands.

M. Joelsson and F. Tjerneld, Purification of Lactate Dehydrogenase from Pig Muscle by Affinity Partitioning.

G. Kopperschlager, Phosphofructokinase from Baker's Yeast.

G. Birkenmeier, Partitioning of Blood Proteins Using Immobilized Dyes.

B.H. Chung, D. Bailey, and F.H. Arnold, Metal Affinity Partitioning.

A. Blennow, Starch Synthase from Potato Tubers.

J.S. Brusca and J.D. Radolf, Isolation of Integral Membrane Proteins by Phase Partitioning with Triton X-114.

Nucleic Acids:

W. Maller, Separation of Proteins and Nucleic Acids.

G.E. Francis and D. Fisher, Purification of Nucleic Acid-Protein Comple


This volume of Methods in Enzymology represents the sole collection of separation methods based on aqueous two-phase systems. It includes procedures for the isolation of proteins, especially enzymes, nucleic acids, cell membranes, and organelles, as well as for the separation and study of cells.

Key Features

@introbul:Key Features @bul:* Use of affinity partitioning for enzyme purification

  • Separation and study of cells
  • Isolation of plasma membranes
  • Large scale biotechnical use


Biochemists, biophysicists, molecular biologists, cell biologists, physical chemists, microbiologists, biotechnologists, plant scientists.


No. of pages:
© Academic Press 1994
Academic Press
eBook ISBN:
Hardcover ISBN:


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About the Serial Volume Editors

Harry Walter Serial Volume Editor

Affiliations and Expertise

Aqueous Phase Systems, Washington, D.C., U.S.A.

Göte Johansson Serial Volume Editor

Affiliations and Expertise

University of Lund, Sweden

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA