This volume of Methods in Enzymology represents the sole collection of separation methods based on aqueous two-phase systems. It includes procedures for the isolation of proteins, especially enzymes, nucleic acids, cell membranes, and organelles, as well as for the separation and study of cells.

Key Features

@introbul:Key Features @bul:* Use of affinity partitioning for enzyme purification * Separation and study of cells * Isolation of plasma membranes * Large scale biotechnical use


Biochemists, biophysicists, molecular biologists, cell biologists, physical chemists, microbiologists, biotechnologists, plant scientists.

Table of Contents

General Methodology and Apparatus: P.-+. Albertsson and F. Tjerneld, Phase Diagrams. D.E. Brooks and R. Norris-Jones, Preparation and Analysis of Two-Phase Systems. G. Johansson, Partitioning Procedures and Techniques: Small Molecules and Macromolecules. H. Walter and C. Larsson, Partitioning Procedures and Techniques: Cells, Organelles, and Membranes. G. Johansson, Uses of Poly(ethylene Glycol) with Charged or Hydrophobic Groups. G. Johansson and M. Joelsson, Systems Containing Organic Solvents. P.-+. Albertsson, Chiral Resolution. H.E. Kerlund and P.-+. Albertsson, Thin-Layer Countercurrent Distribution and Centrifugal Countercurrent Distribution Apparatus. W. Maller, Columns Using Aqueous Two-Phase Systems. P.-O. Larsson, Magnetically Enhanced Phase Separation. Partitioning and Affinity Partitioning of Macromolecules: Proteins: G. Kopperschlager, Affinity Extraction with Dye Ligands. M. Joelsson and F. Tjerneld, Purification of Lactate Dehydrogenase from Pig Muscle by Affinity Partitioning. G. Kopperschlager, Phosphofructokinase from Baker's Yeast. G. Birkenmeier, Partitioning of Blood Proteins Using Immobilized Dyes. B.H. Chung, D. Bailey, and F.H. Arnold, Metal Affinity Partitioning. A. Blennow, Starch Synthase from Potato Tubers. J.S. Brusca and J.D. Radolf, Isolation of Integral Membrane Proteins by Phase Partitioning with Triton X-114. Nucleic Acids: W. Maller, Separation of Proteins and Nucleic Acids. G.E. Francis and D. Fisher, Purification of Nucleic Acid-Protein Comple


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© 1994
Academic Press
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@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY