Approaches to Research on the Systematics of Fish-Borne Trematodes is a concise guide for systematic studies of the prevalence of fish-borne trematodes both in the endemic areas and experimental laboratories. It includes methods to identify species of fish-borne trematodes to enhance the precision of research studies based on the metacercarial stage.

Misidentification of trematode species is a common occurrence when researchers are new to the field and have no guidance. Consequentially, sometimes publications report inaccurate prevalence rates of these parasites. This compact guide gives clear direction on:

  • Collection of parasites in the final hosts
  • Collection of cercaria from snail first intermediate hosts
  • Collection of metacercaria from fish hosts
  • Molecular identification of parasites
  • Systematics of fish-borne trematodes

Key Features

  • Provides research guidelines and protocols for studying systematics of fish-borne trematodes using both morphological and molecular data
  • Presents keys to enable identification of metacercariae of fish-borne trematodes in the Greater Mekong subregion


parasitologists, helminthologists, biologists, ecologists and public health specialists.

Table of Contents


Chapter 1. Medically Important Fish-Borne Zoonotic Trematodes

1.1 Major Fish-Borne Trematodes

1.2 Other Fish-Borne Trematodes


Chapter 2. Collection of Fish-Borne Trematodes from the Final Host

2.1 Diagnostic Methods

2.2 Treatment and Purgation

2.3 Collection of Adult FBT from Animals

2.4 Worm Preparation for Morphological Study

2.5 Diagnostic Characteristics of FBT


Chapter 3. Collection of Fish-Borne Trematode Cercaria

3.1 Snail Host of Fish-Borne Trematodes

3.2 Cercaria Morphological Study

3.3 Morphology of Fresh Cercaria

3.4 Vital Stain

3.5 Chaetotaxy

3.6 Polymerase Chain Reaction


Chapter 4. Collection of Fish-Borne Trematodes in Infective Stage from the Fish: The Second Intermediate Host

4.1 Development of Metacercariae

4.2 Methods of Fish Examination for Metacercariae

4.3 Mobile Laboratory for Fish Examination

4.4 Method of Excysting Metacercariae

4.5 Morphology of Metacercariae


Chapter 5. Molecular Systematics of Fish-Borne Trematodes

5.1 What is Molecular Systematics?

5.2 Species Identifica


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© 2014
Academic Press
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About the authors

Jitra Waikagul

JitraWaikagul has been a specialist in helminthology of the Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Thailand, since 1971. She holds a B.Sc. (Zoology) from Chulalongkorn University, an M.Sc. (Tropical Medicine) from Mahidol University, an M.Sc. (Parasitology) from Queensland University, Australia, and a Ph.D. (Medical Science) from Hiroshima University, Japan. Her research contributes to expanding knowledge of the biology and taxonomy of zoonotic helminthes, in particulars fish- and crustacean-borne tremotodes.

Urusa Thaekham

Urusa Thaenkham has been worked as a specialist in molecular systematics of helminths in the Department of Helminthology, Faculty of Tropical Medicine, Mahidol University, Thailand. She finished her Ph.D. (Tropical Medicine) from Mahidol University. Her research interested is focused on molecular systematics of helminths, in particularly fish- and crustacean-borne trematodes.