Applied Plant Genomics and Biotechnology

Applied Plant Genomics and Biotechnology

1st Edition - January 21, 2015

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  • Authors: P Poltronieri, Yiguo Hong
  • eBook ISBN: 9780081000717
  • Hardcover ISBN: 9780081000687

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Applied plant genomics and biotechnology reviews the recent advancements in the post-genomic era, discussing how different varieties respond to abiotic and biotic stresses, investigating epigenetic modifications and epigenetic memory through analysis of DNA methylation states, applicative uses of RNA silencing and RNA interference in plant physiology and in experimental transgenics, and plants modified to produce high-value pharmaceutical proteins. The book provides an overview of research advances in application of RNA silencing and RNA interference, through Virus-based transient gene expression systems, Virus induced gene complementation (VIGC), Virus induced gene silencing (Sir VIGS, Mr VIGS) Virus-based microRNA silencing (VbMS) and  Virus-based RNA mobility assays (VRMA); RNA based vaccines and expression of virus proteins or RNA, and virus-like particles in plants, the potential of virus vaccines and therapeutics, and exploring plants as factories for useful products and pharmaceuticals are topics wholly deepened. The book reviews and discuss Plant Functional Genomic studies discussing the technologies supporting the genetic improvement of plants and the production of plant varieties more resistant to biotic and abiotic stresses. Several important crops  are analysed providing a glimpse on the most up-to-date methods and topics of investigation. The book presents a review on current state of GMO,  the cisgenesis-derived plants and novel plant products devoid of transgene elements, discuss their regulation and the production of desired traits such as resistance to viruses and disease also in fruit trees and wood trees with long vegetative periods. Several chapters cover aspects of plant physiology  related to plant improvement: cytokinin metabolism and hormone signaling pathways are discussed in barley; PARP-domain proteins involved in Stress-Induced Morphogenetic Response, regulation of NAD signaling and ROS dependent synthesis of anthocyanins. Apple allergen isoforms and the various content in different varieties are discussed and approaches to reduce their presence. Euphorbiaceae, castor bean, cassava and Jathropa are discussed at genomic structure, their diseases and viruses, and methods of transformation. Rice genomics and agricultural traits are discussed, and biotechnology for engineering and improve rice varieties. Mango topics are presented with an overview of molecular methods for variety differentiation, and aspects of fruit improvement by traditional and biotechnology methods. Oilseed rape is presented, discussing the genetic diversity, quality traits, genetic maps, genomic selection  and comparative genomics for improvement of varieties. Tomato studies are presented, with an overview on the knowledge of the regulatory networks involved in flowering, methods applied to study the tomato genome-wide DNA methylation, its regulation by small RNAs, microRNA-dependent control of transcription factors expression, the development and ripening processes in tomato, genomic studies and fruit modelling to establish fleshy fruit traits of interest; the gene reprogramming during fruit ripening, and the ethylene dependent and independent DNA methylation changes.

Key Features

  • provides an overview on the ongoing projects and activities in the field of applied biotechnology
  • includes examples of different crops and applications to be exploited
  • reviews  and discusses Plant Functional Genomic studies and the future developments in the field
  • explores the new technologies supporting the genetic improvement of plants


Researchers, students and practitioners in Biological Sciences, Plant science, Plant biotechnology, Plant physiology and Genetics, Plant functional genomics, Agriculture, Sustainable growth and economy and Bio-based Biochemicals.

Table of Contents

    • List of figures
    • List of tables
    • About the editors
    • About the contributors
    • List of abbreviations
    • Introduction
    • 1. Transgenic, cisgenic and novel plant products: Challenges in regulation and safety assessment
      • 1.1 Genetically modified plant products in the United States
      • 1.2 GMP products in Europe
      • References
    • 2. What turns on and off the cytokinin metabolisms and beyond
      • Acronyms
      • 2.1 Introduction
      • 2.2 Regulation of cytokinin biosynthesis
      • 2.3 Application of altered cytokinin metabolisms to improve agricultural traits
      • Acknowledgement
      • References
    • 3. Apple allergens genomics and biotechnology: Unravelling the determinants of apple allergenicity
      • 3.1 Introduction: Fruit and apple allergies
      • 3.2 Abiotic factors: Influence of environment and cultivation techniques
      • 3.3 Biotic factors: Pathogen infection and allergens content
      • 3.4 Post-harvest, food processing and breeding strategies towards allergenic content decrease
      • 3.5 Conclusion
      • References
    • 4. Non-food interventions: Exploring plant biotechnology applications to therapeutic protein production
      • 4.1 Introduction
      • 4.2 Plant as heterologous expression system for molecular farming
      • 4.3 Stable transformation
      • 4.4 Transient transformation
      • 4.5 Limits on the use of plants as expression systems for molecular farming
      • 4.6 Plant-made recombinant pharmaceuticals
      • 4.7 Regulatory aspects and clinical status of PMPs
      • References
    • 5. In planta produced virus-like particles as candidate vaccines
      • 5.1 Introduction
      • 5.2 Papillomaviruses
      • 5.3 Hepatitis B virus
      • 5.4 Human immunodeficiency virus-1
      • 5.5 Influenza A virus
      • References
    • 6. Biotechnology of Euphorbiaceae (Jatropha curcas, Manihot esculenta, Ricinus communis)
      • 6.1 Euphorbiaceae crops
      • 6.2 Genetic diversity
      • 6.3 Genetic improvement
      • 6.4 Phytosanitary improvement
      • 6.5 Concluding remarks
      • Acknowledgement
      • References
    • 7. Regulation framework for flowering
      • 7.1 Introduction
      • 7.2 Getting ready to flower: the juvenile to adult transition
      • 7.3 Framework controlling flowering
      • 7.4 Flowering sensing the environment
      • 7.5 Endogenous cues regulating flowering
      • 7.6 Flowering time control and manipulation
      • References
    • 8. Epigenetic regulation during fleshy fruit development and ripening
      • 8.1 Introduction
      • 8.2 An overview of DNA methylation in plants
      • 8.3 Histone marks are likely to play fundamental role in fruit development
      • 8.4 Concluding remarks
      • References
    • 9. Tomato fruit quality improvement facing the functional genomics revolution
      • 9.1 Introduction
      • 9.2 What is meant by fruit quality?
      • 9.3 Genomics-assisted breeding for improving fruit quality
      • 9.4 Future potential of tomato breeding using omics approaches
      • 9.5 Fruit modelling to establish fleshy fruit traits of interest
      • 9.6 Concluding remarks
      • References
    • 10. Rice genomics and biotechnology
      • 10.1 Golden age of genomics, biological engineering and paddy rice
      • 10.2 Research on the important agronomic traits in rice biology
      • 10.3 Emergence of new technologies will extend the golden age of paddy rice
      • 10.4 Rice requires further research development to remain a monocotyledon model plant
      • References
    • 11. Genome-wide DNA methylation in tomato
      • 11.1 Introduction
      • 11.2 Methods to detect and quantify DNA methylation
      • 11.3 Distribution of 5-methylcytosine in tomato genome
      • 11.4 Genome-wide DNA methylation reprogramming during fruit ripening
      • 11.5 Conclusion
      • Acknowledgements
      • References
    • 12. Recent application of biotechniques for the improvement of mango research
      • 12.1 Introduction
      • 12.2 Origin and distribution
      • 12.3 Economic importance
      • 12.4 Cytology
      • 12.5 Molecular biotechniques applied on mango
      • 12.6 Application of RAA method on mango
      • 12.7 Problems in mango improvement using biotechnology
      • 12.8 Conclusion and direction of future research
      • References
    • 13. Cotton genomics and biotechnology
      • 13.1 Introduction
      • 13.2 Cotton genomics
      • 13.3 Cotton fibre function genomics
      • 13.4 Cotton biotechnology
      • List of abbreviations
      • References
    • 14. Virus technology for functional genomics in plants
      • Acronyms
      • 14.1 Introduction
      • 14.2 VRMA – virus-based RNA mobility assay (Li et al., 2009, 2011)
      • 14.3 Conclusion
      • References
    • 15. PARP proteins, NAD, epigenetics, antioxidative response to abiotic stress
      • 15.1 Introduction
      • 15.2 Conclusion
      • Acknowledgement
      • References
    • 16. Applied oilseed rape marker technology and genomics
      • 16.1 Global importance of oilseed rape
      • 16.2 Rapeseed yield and quality and potential improvements
      • 16.3 Future potentials of OSR variety and crop improvement
      • 16.4 Conclusion
      • References
    • Index

Product details

  • No. of pages: 356
  • Language: English
  • Copyright: © Woodhead Publishing 2015
  • Published: January 21, 2015
  • Imprint: Woodhead Publishing
  • eBook ISBN: 9780081000717
  • Hardcover ISBN: 9780081000687

About the Authors

P Poltronieri

Dr Palmiro Poltronieri is researcher at the Agrofood Department of the Italian National Research Council. He is co-founder of Biotecgen SME - a service company involved in European projects, such as RIBOREG, NANOMYC, ABSTRESS, and TOMGEM. He has also tutored researchers for another start up, Bioesplora, in the EU project TRANS-BIO. He is Associate Editor to BMC Research Notes and is editor-in-chief for Challenges, an MDPI journal. He holds a Ph.D. in Molecular and Cellular Biology from Verona University. His current interest is on abiotic stress response in roots of tolerant and sensitive chickpea varieties, on activation of the jasmonic acid synthesis, and in the biotic stress response in model plants.

Affiliations and Expertise

Institute of Sciences of Food Production, National Research Council (ISPA-CNR), Lecce, Italy

Yiguo Hong

Professor Yiguo Hong is a scientist based at the College of Life and Environmental Sciences, Hangzhou Normal University, China. He has expertise in plant viruses and RNA silencing, having publications in international journals, such as PNAS, eLife and Nature Genetics. He serves as editors for several international journals such as Functional & Integrative Genomics. He also holds long-term visiting professorship at both University of Warwick and University of Worcester. His current research covers virus-plant interactions, RNA signaling in plant development, and development of virus technology in plant functional genomics and crop improvement.

Affiliations and Expertise

College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou, China; School of Life Science, University of Warwick, Coventry, UK; School of Science and the Environment, University of Worcester. Worcester, UK

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