Applied Biochemistry and Bioengineering

Applied Biochemistry and Bioengineering

Enzyme Technology

First published on January 1, 1979

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  • Editor: Lemuel Wingard
  • eBook ISBN: 9780323156011

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Applied Biochemistry and Bioengineering, Volume 2: Enzyme Technology discusses the industrial applications of immobilized enzymes. Organized into 10 chapters, this volume first describes the techniques for the isolation and purification of intracellular and extracellular enzymes for use on an industrial scale. It then deals with immobilized enzyme processes, with an emphasis on immobilized glucose isomerase and the amylolytic enzymes related to the production of high-fructose syrups from starch. Significant topics on immobilized enzyme technology for future uses in energy transduction and in pharmaceutical modifications of steroid compounds are also explored. Microbiologists, geneticists, and chemical engineers will find this book of great value.

Table of Contents

  • List of Contributors


    Contents of Volume 1

    Production of Intracellular Microbial Enzymes

    I. Introduction

    II. Choice of Microorganism

    III. Influence of Fermentation Conditions on Enzyme Synthesis

    IV. Scale-up of Enzyme Production

    V. Kinetics of Intracellular Enzyme Production

    VI. Continuous-Flow Enzyme Production

    VII. Influence of Fermentation Conditions on Enzyme Isolation

    VIII. Summary


    Production, Isolation, and Economics of Extracellular Enzymes

    I. Introduction

    II. History

    III. The Enzyme Industry

    IV. General Methods for Industrial Production of Extracellular Enzymes

    V. Standardization and Control

    VI. Proteolytic Enzymes

    VII. Amylolytic Enzymes

    VIII. Other Enzymes

    IX. Conclusion


    Extraction and Purification of Enzymes Using Aqueous Two-Phase Systems

    I. Introduction

    II. Incompatibility of Polymers

    III. General Aspects of Aqueous Two-Phase Systems

    IV. Choice of a System

    V. Selection of Parameters

    VI. Influence of the Polymers

    VII. Influence of Ions Included in the Phase System

    VIII. Influence of Temperature

    IX. Influence of the Microorganism

    X. Technical Aspects of Extraction and Separation

    XI. Selectivity of Partition

    XII. Removal of Polymers

    XIII. Conclusions


    Glucose Isomerase Production of High-Fructose Syrups

    I. Introduction

    II. Historical Perspective

    III. Glucose Isomerase

    IV. Commercial Glucose Isomerase Production and Immobilization

    V. Commercial Production of High-Fructose Corn Syrups

    VI. New and Continuing Developments


    Development of an Immobilized Glucose Isomerase for Industrial Application

    I. Introduction

    II. Glucose Isomerization Reaction

    III. Soluble Glucose Isomerase

    IV. Immobilized Glucose Isomerase

    V. Conclusion

    List of Symbols


    Starch Hydrolysis with Soluble and Immobilized Glucoamylase

    I. Introduction

    II. Mode of Action

    III. Physical Properties

    IV. Reaction Mechanism

    V. Reaction of Natural Substrates

    VI. Potential Use of Immobilized Glucoamylase

    VII. Conclusion


    Industrial Applications of Immobilized Enzymes: A Commercial Overview

    I. Introduction

    II. Commercially Available Immobilized Enzyme Technology

    III. Glucose Isomerase

    IV. Glucose Isomerase versus Other Immobilized Systems

    V. Economic Considerations—Immobilized Enzyme Systems

    VI. Options Available for Immobilized Enzyme Development

    VII. Summary and Outlook


    Industrial Applications of Fiber-Entrapped Enzymes

    I. Introduction

    II. Aminoacylase

    III. Fumarase

    IV. Glucoamylase

    V. Glucose Isomerase

    VI. Hydantoinase

    VII. Invertase

    VIII. Lactase

    IX. Multienzyme Systems

    X. Penicillin Amidase

    XI. Tryptophan Synthetase

    XII. Miscellaneous


    Energy-Related Applications of Immobilized Enzymes

    I. Introduction

    II. Water Biophotolysis

    III. Bioelectrocatalysis and Electrode Processes Accelerated by Immobilized Enzymes

    IV. Conclusions


    Transformation of Steroids by Immobilized Living Microorganisms

    I. Introduction

    II. Immobilization Methods

    III. Steroid-Converting Microorganisms Immobilized in Polyacrylamide

    IV. Steroid-Converting Microorganisms Immobilized in Calcium Alginate

    V. General Discussion


    Subject Index

Product details

  • No. of pages: 320
  • Language: English
  • Copyright: © Academic Press 1979
  • Published: January 1, 1979
  • Imprint: Academic Press
  • eBook ISBN: 9780323156011

About the Editor

Lemuel Wingard

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