Apoptosis provides a current and comprehensive collection of methods for the study of cell death. Using a diverse range of technical approaches and model systems, the chapters in this volume cover topics from the cellular and organismal to the molecular and anatomical. The methods are illustrated with user-friendly recipes and over 100 tables, halftones, and diagrams.
- Current methodologies for studying cell death
- Wide range of model systems
- Molecular, biochemical, cellular, and genetic approaches
- Complements the original Cell Death volume
- Up-to-date methodology for a fast moving field
- Designed with the needs of both basic scientists and clinicians in mind
- Authors are leaders in their respective fields
Researchers and graduate students in cell biology, developmental biology, neuroscience, and molecular biology. Academic and research libraries, pharmaceutical and biotechnology companies, and private research facilities.
Contributors. Preface. Isolation and Assay of Caspases, S.M. Srinivasula, A. Saleh, M. Ahmad, T. Fernandes-Alnemri, and E.S. Alnemri. Cloning and Analysis of Bcl-2 Family Genes, E. Cepero, B.W. Johnson, and L.H. Boise. Flow Cytometric Analysis of Cell Shrinkage and Monovalent Ions during Apoptosis, C.D. Bortner and J.A. Cidlowski. Use of flow and Laser-Scanning Cytometry in Analysis of Cell Death, Z. Darzynkiewicz, X. Li, and E. Bedner. Analysis of Protein Transglutamylation in Apoptosis, Z. Nemes, A. Mádi, L.N. Marekov, M. Piacentini, P.M. Steinert, and L. Fés252;s. Analysis of Sphingomyelin and Ceramide Levels and the Enzymes Regulating their Metabolism in Response to Cell Stress, R.T. Dobrowsky and R.N. Kolesnick. Cell-Free Systems to Study Apoptosis, H.O. Fearnhead. Role of c-Jun N-terminal Kinase in Apoptosis, Z.-g. Liu, J. Lewis, T.-H. Wang, and A. Cook. Methods for Studying Pro- and Antiapoptotic Genes in Nonimmortal Cells, M.E. McCurrach and S.W. Lowe. Calcium Flux Measurements in Apoptosis, D.J. McConkey and L.K. Nutt. Proteinase Families and Their Inhibitors, D.L. Mykles. Identification and Analysis of Caspase Substrates: Proteolytic Cleavage of Poly (ADP-ribose)polymerase and DNA Fragmentation Factor 45, C. Boucher, S. Gobeil, K. Samejima, W.C. Earnshaw, and G.G. Poirier. Analysis of Reactive Oxygen Species in Cell Death, I. Stamenkovic. Methods for Studying Apoptosis and Phagocytosis of Apoptotic Cells in Drosophila Tissues and Cell Lines, K. White, S. Lisi, P. Kurada, N. Franc, and P. Bangs. Phosphatidylserine Exposure and Phagocytosis of Apoptotic Cells, P. Williamson, S. van den Eijnde, and R.A. Schle
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- © Academic Press 2001
- 21st May 2001
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
University of Massachusetts, Department of Biology, Amherst, U.S.A.
Laboratory of Immune Cell Biology, Maryland, U.S.A.
Praise for the First Edition "This is a high quality book and should be useful for several years to come. (It is) a very useful book that should be in every research library. The research effort investigating PCD is growing at a very rapid rate and this book is a timely addition to this important research area. (I rate it at) five out of five stars." --DOODY'S JOURNAL