1st Edition - July 5, 2000
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  • Editors-in-Chief: John Abelson, Melvin Simon
  • eBook ISBN: 9780080496771

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Volume 322 of Methods in Enzymology is dedicated to apoptosis. Major topics covered include measuring apoptosis and apoptosis-induced endonucleases, measuring apoptosis in lower organisms, proteases involved in apoptosis and their inhibitors, cell free systems for monitoring steps in apoptosis pathways, mitochondria and apoptosis, bCl-2 family proteins, and studying receptors and signal transduction events implicated in cell survival and cell death.The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.


Biochemists, cell biologists, molecular biologists, microbiologists, and biomedical researchers.

Table of Contents

  • Contributors to Volume 322


    Methods in Enzymology

    Detection of DNA Cleavage in Apoptotic Cells

    Detection of Apoptosis by Annexin V Labeling

    Analysis of Apoptotic Cells by Flow and Laser Scanning Cytometry

    Quantitative Measurement of Apoptosis Induced by Cytotoxic T Lymphocytes

    Apoptotic Nuclease Assays

    Analysis of Programmed Cell Death and Apoptosis in Drosophila

    Analysis of Programmed Cell Death in the Nematode Caenorhabditis elegans

    Caspase Assays

    Determination of Caspase Specificities Using a Peptide Combinatorial Library

    Criteria for Identifying Authentic Caspase Substrates during Apoptosis

    Purification and Use of Granzyme B

    Viral Caspase Inhibitors CrmA and p35

    Purification and Use of Recombinant Inhibitor of Apoptosis Proteins as Caspase Inhibitors

    Monitoring Activity of Caspases and Their Regulators in Yeast Saccharomyces cerevisiae

    In Vitro Assays for Caspase-3 Activation and DNA Fragmentation

    Cell-Free Apoptosis in Xenopus laevis Egg Extracts

    Cytofluorometric Quantitation of Nuclear Apoptosis Induced in a Cell-Free System

    Purification of Mitochondria for Apoptosis Assays

    Quantitation of Mitochondrial Transmembrane Potential in Cells and in Isolated Mitochondria

    Nitrogen Cavitation for Cell Disruption to Obtain Mitochondria from Cultured Cells

    Apoptosis-Related Activities Measured with Isolated Mitochondria and Digitonin-Permeabilized Cells

    Assays for Cytochrome c Release from Mitochondria during Apoptosis

    Purification and Liposomal Reconstitution of Permeability Transition Pore Complex

    Monitoring Interactions of Bcl-2 Family Proteins in 96-Well Plate Assays

    Analysis of Dimerization of Bcl-2 Family Proteins by Surface Plasmon Resonance

    Measuring Pore Formation by Bcl-2 Family Proteins

    Assays for Studying Bax-Induced Lethality in the Yeast Saccharomyces cereνisiae

    Exploiting the Utility of Yeast in the Context of Programmed Cell Death

    Production of Recombinant TRAIL and TRAIL Receptor: Fc Chimeric Proteins

    Expression of Lymphotoxins and Their Receptor–Fc Fusion Proteins by Baculovirus

    Analysis of the CD95 (APO-1/Fas) Death-Inducing Signaling Complex by High-Resolution Two-Dimensional Gel Electrophoresis

    Measurement of Ceramide Levels by the Diacylglycerol Kinase Reaction and by High-Performance Liquid Chromatography–Fluorescence Spectrometry

    Measurement of Ceramide Synthase Activity

    Measurement of Sphingomyelinase Activity

    Assays for JNK and p38 Mitogen-Activated Protein Kinases

    Assaying for IκB Kinase Activity

    Assays for Akt

    Measurement of Cellular Oxidation, Reactive Oxygen Species, and Antioxidant Enzymes during Apoptosis

    Volume Regulation and Ion Transport during Apoptosis

    Assays for Transglutaminases in Cell Death


    Transient Transfection Assay of Cell Death Genes

    Sindbis Virus Vector System for Functional Analysis of Apoptosis Regulators

    Transduction of Full-Length Tat Fusion Proteins Directly into Mammalian Cells: Analysis of T Cell Receptor Activation-Induced Cell Death

    Author Index

    Subject Index

Product details

  • No. of pages: 569
  • Language: English
  • Copyright: © Academic Press 2000
  • Published: July 5, 2000
  • Imprint: Academic Press
  • eBook ISBN: 9780080496771

About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editor

John Reed

Affiliations and Expertise

The Burnham Institute, La Jolla, California, U.S.A.