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Contributors to Volume 322
Methods in Enzymology
Detection of DNA Cleavage in Apoptotic Cells
Detection of Apoptosis by Annexin V Labeling
Analysis of Apoptotic Cells by Flow and Laser Scanning Cytometry
Quantitative Measurement of Apoptosis Induced by Cytotoxic T Lymphocytes
Apoptotic Nuclease Assays
Analysis of Programmed Cell Death and Apoptosis in Drosophila
Analysis of Programmed Cell Death in the Nematode Caenorhabditis elegans
Determination of Caspase Specificities Using a Peptide Combinatorial Library
Criteria for Identifying Authentic Caspase Substrates during Apoptosis
Purification and Use of Granzyme B
Viral Caspase Inhibitors CrmA and p35
Purification and Use of Recombinant Inhibitor of Apoptosis Proteins as Caspase Inhibitors
Monitoring Activity of Caspases and Their Regulators in Yeast Saccharomyces cerevisiae
In Vitro Assays for Caspase-3 Activation and DNA Fragmentation
Cell-Free Apoptosis in Xenopus laevis Egg Extracts
Cytofluorometric Quantitation of Nuclear Apoptosis Induced in a Cell-Free System
Purification of Mitochondria for Apoptosis Assays
Quantitation of Mitochondrial Transmembrane Potential in Cells and in Isolated Mitochondria
Nitrogen Cavitation for Cell Disruption to Obtain Mitochondria from Cultured Cells
Apoptosis-Related Activities Measured with Isolated Mitochondria and Digitonin-Permeabilized Cells
Assays for Cytochrome c Release from Mitochondria during Apoptosis
Purification and Liposomal Reconstitution of Permeability Transition Pore Complex
Monitoring Interactions of Bcl-2 Family Proteins in 96-Well Plate Assays
Analysis of Dimerization of Bcl-2 Family Proteins by Surface Plasmon Resonance
Measuring Pore Formation by Bcl-2 Family Proteins
Assays for Studying Bax-Induced Lethality in the Yeast Saccharomyces cereνisiae
Exploiting the Utility of Yeast in the Context of Programmed Cell Death
Production of Recombinant TRAIL and TRAIL Receptor: Fc Chimeric Proteins
Expression of Lymphotoxins and Their Receptor–Fc Fusion Proteins by Baculovirus
Analysis of the CD95 (APO-1/Fas) Death-Inducing Signaling Complex by High-Resolution Two-Dimensional Gel Electrophoresis
Measurement of Ceramide Levels by the Diacylglycerol Kinase Reaction and by High-Performance Liquid Chromatography–Fluorescence Spectrometry
Measurement of Ceramide Synthase Activity
Measurement of Sphingomyelinase Activity
Assays for JNK and p38 Mitogen-Activated Protein Kinases
Assaying for IκB Kinase Activity
Assays for Akt
Measurement of Cellular Oxidation, Reactive Oxygen Species, and Antioxidant Enzymes during Apoptosis
Volume Regulation and Ion Transport during Apoptosis
Assays for Transglutaminases in Cell Death
Transient Transfection Assay of Cell Death Genes
Sindbis Virus Vector System for Functional Analysis of Apoptosis Regulators
Transduction of Full-Length Tat Fusion Proteins Directly into Mammalian Cells: Analysis of T Cell Receptor Activation-Induced Cell Death
Volume 322 of Methods in Enzymology is dedicated to apoptosis. Major topics covered include measuring apoptosis and apoptosis-induced endonucleases, measuring apoptosis in lower organisms, proteases involved in apoptosis and their inhibitors, cell free systems for monitoring steps in apoptosis pathways, mitochondria and apoptosis, bCl-2 family proteins, and studying receptors and signal transduction events implicated in cell survival and cell death. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Biochemists, cell biologists, molecular biologists, microbiologists, and biomedical researchers.
- No. of pages:
- © Academic Press 2000
- 5th July 2000
- Academic Press
- Hardcover ISBN:
- eBook ISBN:
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California Institute of Technology, Division of Biology, Pasadena, U.S.A.
The Salk Institute, La Jolla, CA, USA
The Burnham Institute, La Jolla, California, U.S.A.
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