Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods which inhibit gene expression permit studies probing the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmacology, oncology, genetics, cell biology, developmental biology, molecular biology, biochemistry, and neurosciences. This volume will be a truly important tool in biomedically-oriented research. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.
Biochemists, geneticists, pharmacologists, oncologists, cell biologists, developmental biologists, molecular biologists, biomedically oriented researchers, neuroscientists, and physiologists.
Antisense Receptor Targets:
J. Garzón, I. de Antonio, and P. Sánchez-Blázquez, In vivo Modulation of G-proteins and Opioid Receptor Function by Antisense Oligodeoxynucleotides.
J. Karle and M. Nielsen, Targeting Brain GABA-A Receptors with Antisense Oligonucleotides: Implications for Epilepsy.
D. Mohuezy, X. Tang, and M.I. Phillips, Delivery of Antisense DNA by Vectors for Prolonged Effects in Vitro and in Vivo.
G.W. Pasternak and Y.-X. Pan, Antisense Mappling: Assessing the Functional Significance of Genes and Splice Variants.
E.S.J. Robinson and A.L. Hudson, In Vivo and in Vitro Effects of Antisense on Þ2-Adrenoceptor Expression.
J.G. Hensler and J.M. Scalzitti, Design and Efficacy of a Seritonin2A Receptor Antisense Oligodeoxynucleotide.
M. Beinfeld and D. Vishnuvardham, The Use of Expression of Antisense mRNA for the Enzymes PC1 and PC2 in Prohormone Processing.
Antisense Neuroscience Targets:
W.C. Broaddus, S.S. Prabhu, S. Wu-Pong, G.T. Gillies, and H. Fillmore, Design and Testing of Antisense Oligonucleotide Strategies for the Central Nervous System.
N.J. Buckley, Fe.C. Abogadie, D.A. Brown, M. Dayrell, M.P. Caulfield, P. Delmas, and J.E. Haley, Use of Antisense Expression Plasmids to Attenuate G-Expression in Primary Neurons.
S. Catsicas, F. Hallböök, M. Catsicas, J.K. Staple, and G. Grenningloh, Gene Functional Analysis in the Nervous System.
S. Peng Ho, D.H.O. Britton, Y. Bao, and M.S. Scully, RNA Mapping: Selection of Potent Oligonucleotide Sequences for the Antisense Experiment.
P.S. Kalra, M.G. Dube, and S.P. Kalra, Effects of Centrality Administered Antisense Oligodeoxynucleotide
- No. of pages:
- © Academic Press 2000
- 22nd October 1999
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
University of Florida, Gainesville, U.S.A. Norris Professor of Life Sciences
@from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY