Antisense Technology, Part B: Applications, Volume 314

1st Edition

Editor-in-Chiefs: John Abelson Melvin Simon
Serial Volume Editors: M. Ian Phillips
Hardcover ISBN: 9780121822156
eBook ISBN: 9780080496719
Imprint: Academic Press
Published Date: 22nd October 1999
Page Count: 647
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Table of Contents

Antisense Receptor Targets:

J. Garzón, I. de Antonio, and P. Sánchez-Blázquez, In vivo Modulation of G-proteins and Opioid Receptor Function by Antisense Oligodeoxynucleotides.

J. Karle and M. Nielsen, Targeting Brain GABA-A Receptors with Antisense Oligonucleotides: Implications for Epilepsy.

D. Mohuezy, X. Tang, and M.I. Phillips, Delivery of Antisense DNA by Vectors for Prolonged Effects in Vitro and in Vivo.

G.W. Pasternak and Y.-X. Pan, Antisense Mappling: Assessing the Functional Significance of Genes and Splice Variants.

E.S.J. Robinson and A.L. Hudson, In Vivo and in Vitro Effects of Antisense on Þ2-Adrenoceptor Expression.

J.G. Hensler and J.M. Scalzitti, Design and Efficacy of a Seritonin2A Receptor Antisense Oligodeoxynucleotide.

M. Beinfeld and D. Vishnuvardham, The Use of Expression of Antisense mRNA for the Enzymes PC1 and PC2 in Prohormone Processing.

Antisense Neuroscience Targets:

W.C. Broaddus, S.S. Prabhu, S. Wu-Pong, G.T. Gillies, and H. Fillmore, Design and Testing of Antisense Oligonucleotide Strategies for the Central Nervous System.

N.J. Buckley, Fe.C. Abogadie, D.A. Brown, M. Dayrell, M.P. Caulfield, P. Delmas, and J.E. Haley, Use of Antisense Expression Plasmids to Attenuate G-Expression in Primary Neurons.

S. Catsicas, F. Hallböök, M. Catsicas, J.K. Staple, and G. Grenningloh, Gene Functional Analysis in the Nervous System.

S. Peng Ho, D.H.O. Britton, Y. Bao, and M.S. Scully, RNA Mapping: Selection of Potent Oligonucleotide Sequences for the Antisense Experiment.

P.S. Kalra, M.G. Dube, and S.P. Kalra, Effects of Centrality Administered Antisense Oligodeoxynucleotide


Antisense technology is the ability to manipulate gene expression within mammalian cells providing powerful experimental approaches for the study of gene function and gene regulation. For example, methods which inhibit gene expression permit studies probing the normal function of a specific product within a cell. Such methodology can be used in many disciplines such as pharmacology, oncology, genetics, cell biology, developmental biology, molecular biology, biochemistry, and neurosciences. This volume will be a truly important tool in biomedically-oriented research. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today-truly an essential publication for researchers in all fields of life sciences.


Biochemists, geneticists, pharmacologists, oncologists, cell biologists, developmental biologists, molecular biologists, biomedically oriented researchers, neuroscientists, and physiologists.


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About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editors

M. Ian Phillips Serial Volume Editor

Affiliations and Expertise

University of Florida, Gainesville, U.S.A. Norris Professor of Life Sciences