Antibodies in Cell Biology focuses on a new generation of protocols aimed at the cell biologist. This laboratory manual features systems and techniques that are especially relevant for modern problems. The contributing authors have been carefully chosen for their specific expertise, and have provided detailed protocols, recipes, and troubleshooting guides in each chapter. The book is designed for any researcher or student who needs to use antibodies in cell biology and related research areas.

Key Features

@introbul:Key Features @bul:* Practical applications and future emphases of antibodies, including: * Light microscopic immunolocalization of antigens * Gold particles in immunoelectron microscopy * Special methods of fixation and permeabilization * Microinjection of antibodies into living cells * Antibodies to identify cDNA clones * Antisense antibody strategies


New and experienced researchers, graduate students, and technicians in cell and developmental biology, immunology, biotechnology, biochemistry, genetics and molecular biology, and other biological areas in which antibodies are used for research.

Table of Contents

E.S. Golub, The Immune Response: An Overview. D. Drenckhahn, T. Jöns, and F. Schmitz, Production of Polyclonal Antibodies Against Proteins and Peptides. D.J. Asai and J.K. Wilder, Making Monoclonal Antibodies. T.J. Smith, Purification of Mouse Antibodies and Fab Fragments. W.-J.Y. Tang, Blot-Affinity Purification of Antibodies. J.J. Otto, Immunoblotting. J.J. Otto and S.-W. Lee, Immunoprecipitation Methods D. Thrower, M.A. Jordon, and L. Wilson, A Quantitative Solid-Phase Binding Assay for Tubulin. D.L. Gard and D.L. Kropf, Confocal Immunofluorescence Microscopy of Microtubules in Oocytes, Eggs, and Embryos of Algae, and Amphibians. S.M. Wick, Immunolabeling of Antigens in Plant Cells. C.E. Alfa, I.M. Gallagher, and J.S. Hyams, Antigen Localization in Fission Yeast. B.D. Wright and J.M. Scholey, Non-Fluorescent Immunolocalization of Antigens in Mitotic Sea Urchin Blastomeres. P. van de Plas and J.L.M. Leunissen, Ultrasmall Gold Probes: Characteristics and Use in Immuno(cyto)chemical Studies. H.C. Joshi, Immunogold Electron Microscopy: Mapping Tubulin Isotypes on Neurite Microtubules. P.A. Erickson, G.P. Lewis, and S.K. Fisher, Postembedding Immunocytochemical Techniques for Light and Electron Microscopy. J.Z. Kiss and K. McDonald, Electron Microscopy Immunocytochemistry Following Cryofixation and Freeze Substitution. B.M. Jockusch and C.J. Temm-Grove, Microinjection of Antibodies in the Analysis of Cellular Architecture and Motility. R.A. Obar and E.L.F. Holzbaur, Immunoselection and Characterization of cDNA Clones. S.D. Georgatos, Antiidiotypic Antibodies: Method


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© 1993
Academic Press
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