COVID-19 Update: We are currently shipping orders daily. However, due to transit disruptions in some geographies, deliveries may be delayed. To provide all customers with timely access to content, we are offering 50% off Science and Technology Print & eBook bundle options. Terms & conditions.
Annotating New Genes - 1st Edition - ISBN: 9781907568688, 9781908818126

Annotating New Genes

1st Edition

From in Silico Screening to Experimental Validation

0.0 star rating Write a review
Author: Shizuka Uchida
Hardcover ISBN: 9781907568688
eBook ISBN: 9781908818126
Imprint: Woodhead Publishing
Published Date: 6th August 2012
Page Count: 196
Sales tax will be calculated at check-out Price includes VAT/GST
Price includes VAT/GST

Institutional Subscription

Secure Checkout

Personal information is secured with SSL technology.

Free Shipping

Free global shipping
No minimum order.

Table of Contents

List of figures and tables


About the author

Chapter 1: Introduction

Chapter 2: Databases and software to make your research life easier


2.1 Introduction

2.2 Primary databases

2.3 Secondary databases

2.4 Datasets to keep an eye out for

2.5 Conclusion

Chapter 3: How to program like a bioinformatician?


3.1 Introduction

3.2 Setting up an environment

3.3 Writing Perl scripts

3.4 Conclusion

Chapter 4: Case study: C-It, knowledge database for screening evolutionarily conserved, tissue-enriched, uncharacterized genes


4.1 Introduction

4.2 Concept of in silico screening

4.3 Case study: C-It

Chapter 5: Experimental validation: concepts and practice


5.1 Introduction

5.2 Proposed experimental strategy

5.3 Step 1: validation of expression patterns

5.4 Step 2: phenotypic analysis

5.5 Step 3: molecular interactions

5.6 Other experiments

5.7 Conclusion

Chapter 6: Further application: adult stem cells and tissue regenerations


6.1 Introduction

6.2 Cell types in heart and their lineages

6.3 Current status of cardiac stem cells

6.4 Conclusion

Chapter 7: Conclusion



In recent years, a number of academic and commercial software packages and databases have been developed for the analysis and screening of biological data; however, the usability of these data is compromised by so-called novel genes to which no biological function is assigned. Annotating new genes outlines an approach to the analysis of evolutionary-conserved, heart-enriched genes with unknown functions, offering a step-by-step description of the procedure from screening to validation. The book begins by offering an introduction to the databases and software available, before moving on to cover programming guidelines, including a specific case study on the use of C-It for in silico screening. The second half of the book offers a step-by-step guide to experimental validation concepts and procedures, as well as an overview of additional potential applications of this approach in the field of stem cells and tissue regeneration, before a concluding chapter summarises the concepts and theories presented.

Key Features

  • Focuses not only on screening but also on biological validations
  • Provides details of databases and software (web interface) products for biologists with minimal computation skills
  • Offers a step-by-step outline of the procedure involved


Academics and researchers in the field of bioinformatics and developmental biology; Geneticists and biologists


No. of pages:
© Woodhead Publishing 2012
6th August 2012
Woodhead Publishing
Hardcover ISBN:
eBook ISBN:

Ratings and Reviews

About the Author

Shizuka Uchida

Shizuka Uchida is a group leader in the Max-Planck Institute for Heart and Lung Research in Bad Nauheim, Germany. He is a trained bioinformatician and a developmental biologist with a specific focus on adult stem cell research, and has extensive experience in combining the power of computers and data mining methods with state-of-the-art experimental techniques.

Affiliations and Expertise

Max-Planck-Institute, Germany