Animal Cell Technology

Animal Cell Technology

Products of Today, Prospects for Tomorrow

1st Edition - January 1, 1994

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  • Editors: R. E. Spier, J. B. Griffiths, W. Berthold
  • eBook ISBN: 9781483162935

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Animal Cell Technology: Products of Today, Prospects for Tomorrow is a collection of papers that discusses the advancement and future of biotechnology. The book presents a total of 164 materials that are organized into 22 sections. The coverage of the text includes the various methodologies involved in animal cell technology, such as post translational modifications; kinetics and modeling; and measurement and assay. The book also covers product safety and consistency testing; products from animal cells in culture; and apoptosis and cell biology. The text will be of great use to biologists, biotechnicians, and biological engineers. Readers who have an interest in the advancement of biotechnology will also benefit from the book.

Table of Contents

  • Section 1. Chairman's Statement

    Presence and Future of Animal Cell Technology

    Section 2. Making and Selecting Recombinant Cell Lines

    Introduction of DNA Into Mammalian Cells

    Retrotargeting: Use of Defective Retroviral DNA Fragments to Improve Recombinant Protein Production in Mammalian Cells

    Receptor-Mediated Gene Delivery into Mammalian Cells

    Improvement of Cell Lines for Large Scale Mammalian Cell Culturing

    Drosophila Cell Lines as Hosts for Recombinant Protein Expression

    Immortalized Cell Lines for Virus Diagnosis

    Selection Strategies for Highly Productive Recombinant Cell Lines

    Section 3. Cell Line Characterization

    Population Analysis of a Recombinant Chinese Hamster Ovary Cell Line

    Expressing Recombinant Human Protein Cultured in the Presence and Absence of Methotrexate Selective Pressure

    Genetic and Biochemical Analysis of a Murine Hybridoma in Long-Term Continuous Culture

    DNA Fingerprinting as a Quality Control Marker for the Genetic Stability of Production Cells

    Analysis of SV40 Early Region Expression in Immortalized Mouse Macrophage Cell Lines

    Section 4. Complex Media formulations

    Stimulation of Murine Hybridomas by Cytokines

    The Use of 2-Hydroxy-2,4,6-Cycloheptarin-1-One (Tropolone) as a Replacemetn for Transferrin

    Comparison of Long R3IGF-1 with Insulin in the Support of Cell Growth and Recombinant Protein Expression in CHO Cells

    Development of a Hybridoma Cloning Medium

    Performance of Gamma-Irradiated Fetal Bovine Serum in Cell Culture

    Low Level FCS Adaptation and Media Development for the Culture of Two Hybridoma Cell Lines Producing Igg and IGM Monoclonal Antibodies

    Improved Bioreactor Productivity and Manufacturing Efficiency Using Liquid Medium Concentrates

    Cell Culture Raw Materials Screening by Calcein-AM Fluorescence Using a 96 Well Plate format

    Importance of Extra-Cellular Matrix in the Long Term Anchorage Dependent Growth of Cell Line CHO K1

    Section 5. Protein and or Serum Free Cultures

    Development of Serum Free Media for Engineered NSO Cell Lines

    Chloresterol: An Important Additive for Serum-Free Media?

    Serum Free Ultraculture? Medium: An Alternative to the Instability Generated by Long-Term Cultivation of Mouse Hybridomas

    Investigations of the TSH Antibody Producing Mouse Hybridoma Cell Line under Serum-Free Media Condition

    Interferon Gamma Production in Laboratory Bioreactor by Lens Culinaris Lectin Stimulated Human Buffy Coat Using Protein Free Medium

    Evaluation of CHO Cell Growth in a Protein-Free Environment

    Inactivation of Serum Contaminants

    Adaption of Mammalian Cells to Protein-Free Growth

    Section 6. Ammonia/Glutamine and other Inhibitors

    Ammonium Ions Inhibit Cell Growth by Intracellular formation of UDP-Activated Hexosamines

    Influence of the PH on the Ammonia Sensitivity of a Murine Hybridoma Cell Line

    The Influence of Ammonium on T-Pa Production from Chinese Hamster Ovary (CHO) Cells

    Detoxification of Ammonium in Bioreactor Cultivations

    Influence of Lactate and Ammonia on the Death Rate of Hybridoma

    The Effect of Glutamine-Containing Dipeptides on the Growth and Productivity of a Hybridoma

    Inhibitory Substance(S) Secreted in Cell Culture Media of Recombinant CHO and a Hybridoma Cell Line

    Growth of Hybridoma Cells is Inhibited by Gangliosides

    Section 7. Apoptosis and Cell Biology

    Programmed to Die: Cell Death via Apoptosis

    Cell Death (Apoptosis) in Cell Culture

    Cell Death by Necrosis and Adoptosis during the Culture of Commercially Important Cell Lines

    Spontaneous Apoptosis Contributes Negatively to the Regulation of Hybridoma Cell Viability

    Metabolic Characteristics and Specific Requests of Hybridoma and CHO Cell Lines

    Nature of Cell-Cell Interactions Between Recombinant CHO Cells Expressing Human Interferon Gamma

    Section 8. Perfusion Cultures and Segregation Methods

    High Density Insect Cell Culture for the Production of Recombinant Proteins with the Baculovirus Expression System

    The Super-Spinner: A Mass Cell Culture Bioreactor for the CO2 Incubator

    Continuous Suspension CHO Cell Culture

    Progress in Animal Cell Perfusion Technology with Superspin

    Production of a Recombinant Protein in a High Density Insect Cell Cytoflow Reactor

    A Compact Gravitational Settling Device for Cell Retention

    Conical Bioreactor with Internal Lamella Settler for Perfusion Culture of Suspension Cells

    Lamellar Clarifier - A New Device for Animal Cell Retention in Perfusion Culture Systems

    Perfusion Culture of Suspended CHO Cells Employing Inclined Sedimentation

    Section 9. Fixed and Fluidized Beds

    Collegenase Production by a Cell Line in Collagen Microspheres in a Fluidized-Bed Reactor and Its Modelization

    Development of a Bioreactor Process for Recombinant Cell Lines

    Growth and Metabolism of CHO-Cells in Porous Glass Carriers

    Evolution of the Capacitance of Cytodex 3 Microbeads Suspended in Growth Medium

    Section 10. Porous Microcarriers

    High Density Culture Using Macroporous Microcarrier

    Growth of Spodoptera Cells on Porous Microcarriers (Verax) Production of Recombinant Proteins

    Protein Free Culture of R-CHO and Hybridoma Cells and Macroporous PolyporeR Carrier

    Section 11. Hollow Fiber Bioreactors

    A Novel Hepatocyte Entrapment, Hollow Fiber Bioreactor as a Bio-Artificial Liver

    Pitfalls of Bioprocessing a Human Monoclonal Multireactive IGM Antibody

    Hollow Fiber Cell Culture - A Proven Alternative

    Section 12. Novel Bioreactors

    Development of a New Membrane Reactor for Large Scale Mammalian Cell Culture

    A Simple Magnetic Driven Mini-Reactor for Fluidized and Packed Beds

    Animal Cell Cultivation in the NASA Rotating-Wall Vessel

    Human Cancer and Primary Cell Culture in the New Hybrid Bioreactor System Tecnomouse

    Analysis of a Long Term Hybridoma Culture in a New Minimized High Cell Density Bioreactor

    Cell-Bubble Interactions During Aeration are Strongly Influenced by Surfactants in the Medium and Can be Minimized in the Newly Developed Bubble Bed Reactor

    Micro-Bubble Sparging of Anchorage Dependent Animal Cell Cultures

    Rationalization of the Design of High Density Perfusion Cultures for Suspended Animal Cells

    Simulation of Hybridoma Growth and Monoclonal Antibody Production in a Homogenous Dialysis Bioreactor

    Section 13. Bioreactor Comparisons

    Comparison of Biorector Technologies for Efficient Production of Recombinant α-Amidating Enzyme

    A Comparison of Animal Cell Growth Using Microcarriers and Suspended Natural Aggregates

    Comparative Studies of Cell Propagation Systems for Production of Human Acetylcholinesterase by Recombinant Cells

    Production of a Monoclonal Antibody with a Serum Dependant Hybridoma in Different Reactor Systems

    Transient Expression with Cos Cells on Spinner Scale

    Production of The HIV-1 Neutralizing Human Monoclonal Antibody 2F5: Stirred Tank versus Fluidized Bed Culture

    Section 14. Novel Measurements and Assays

    The CytosensorTM-Microphysiometer: Biological Applications of Living Cells on Silicon Sensor

    Mass Spectroscopy Determination of Insect Cell Respiration Rates in Culture and Production Processes

    Intracellular PH Evolution During Batch Cultures Using Flow Cytometry

    Cell Cycle Analysis as a Tool for Control and Regulation of Mammalian Cell Cultures in Bioreactors

    Use of MTT-Assay in Studies of the CHO: DG44 Cell Line

    On-line-Measurement of Metabolites in Mammalian Cell Bioreactors by Means of Enzyme Electrodes and of the Number of Living and Dead Cells

    Fully Automated Image Analysis of Cell Number, Viability and Morphology

    Fast Quantification of Mouse IgG from Cell Culture Supernatants

    On-Line Monitoring of Growth and Viral Infection of Vero Cells in Collagen Microspheres in a Fluidized-Bed Bioreactor

    On-Line Glucose Control of Animal Cell Cultures in Fluidized Beds

    On-Line Indirect Measurement of Oxygen Uptake Rate in Bench Scale Hybridoma Cultures

    Software Sensors for the Monitoring of High-Density Hybridoma Perfusion Cultures

    Application of an Industrial Disc Stack Centrifuge for the Separation of Hybridoma Cell as a First Step in Downstream Processing

    Section 15. Physical Factors in Bioreactors

    Dynamic Interfacial Tension and Theological Properties of Cell Culture Medium with Shear Protectant Additives

    Mixing Phenomena in Industrial Bioreactors with Perfusion Spin Filters

    Hydrodynamic Control of Suspended Natural Animal Cell Aggregates

    Modeling and Prediction of Animal Cell Disruption in Simple Flows

    Biosynthetic Responses and Scanning Electron Microscopic Studies of Murine Hybridomas Subjected to Hydrodynamic Stresses

    Section 16. Bioreactor Operation Strategies

    Fed-Batch Strategies for Mammalian Cell Cultures

    Growth and Productivity of Animal Cells: A Contribution to Some Factors Influencing Both

    Simultaneous Control of Growth and Productivity Using a Mutant CHO Cell Line

    Factors Affecting Growth, Infection and Protein Yield in Baculovirus-Infected Insect Cells

    Effects of the Feed Medium Glucose Concentration on Growth and Metabolism of Hybridomas in Chemostat Culture

    Effects of Glucose on the Production of Recombinant Protein C in Mammalian Cell Culture

    Substrate Level Control in Fed-Batch Hybridoma Cultures

    Effect of Dissolved Oxygen on Monoclonal Antibody Production from Hybridoma Cultured in Haemodialysers

    Metabolic Parameters of a Hybridoma Cell Line in Batch and Continuous Cultivation

    Overproduction of Monoclonal Antibodies in High Salt Medium in the Presence of Butyrate

    Growth Control of Production Processes with BHK Cells and Regulation of the Perfusion Rate by Monitoring the Intracellular Nucleotide Pools

    Control of The Maximal Cell Density in a Membrane Perfusion Reactor

    Continuous Medium Recycling In Pilot Scale with a Recombinant Animal Cell Culture for Antithrombin III Production

    Optimization of Antibody Production in a Fluidized Bed Bioreactor

    Monoclonal Antibodies Released from Viable Hybridoma Cells at Different Stages of Growth

    High Cell Density in Insect Cell Cultures

    Semi-Continuous Production with the Baculovirus Expression System

    Long Term Cultures of BHK Suspended Aggregates

    Section 17. Kinetics and Modeling

    Batch Kinetic Data of Hybridoma Growth and Productivity as a Basis for Simulation of Antibody Production in Different Culture Systems

    Modeling Insect Cell Cultures Infected with Recombinant Baculovirus

    Kinetic Analysis of Hybridoma Growth and Monoclonal Antibody Production in Semicontinuous and Continuous Cultures with Respect to Batch Cultures

    Process Development and Kinetic Analysis of a Protein-Free Hybridoma Perfusion System

    Steady-State Analysis of Hybridomas in Chemostta Culture

    Kinetics Studies of Fed-Batch Hybridoma Cultures: Effect of Various Feeding Compositions and Flow Rates

    A General Two-Step Procedure for the Kinetic Modeling of Animal Cell Cultures

    Mathematical Modeling of the Growth and Production Kinetics in Hybridoma Cultures

    Section 18. Downstream Processing

    Impact of Improved Chromatographic Media on Productivity and Process Design in Downstream Processing

    Direct Capture of Recombinant Proteins from Animal Cell Culture Using a Fluidized Bed

    Chromatographic Separation of Immunoglobins on a New "Tentacle"-Type "TA"-Phase: Fractogel EMD TA 650(S)

    Validation of Chromatographic Procedures Used in the Fractionation of Plasma Derivatives

    Section 19. Products from Animal Cells in Culture

    Successful Products and Future Business Prospects

    Production of Recombinant Factor VIII from Perfusion Cultures: I Large-Scale Fermentation

    Production of Recombinant Factor VIII from Perfusion Cultures: II Large-Scale Purification

    Advantages of High Cell Density In Vitro Cultures in Down Stream Processing of Monoclonal IGG Antibody for In Vivo Use

    Production, Purification and Application of Flatfish Interferon

    Monoclonal Antibody Production Strategy for Serum Dependent Clone

    High Level Measles Virus N Protein Expression Using a Recombinant Baculovirus: Assembly of Nucleocapsid-Like Structures

    Equimolar Expression of Two Protein Chains in Recombinant Mammalian Cells

    Expression of Mammalian Protein Farnesyltransferase in a Baculovirus System

    Levels of Troponin I and Myosin Heavy Chain in Differentiated Adult Rabbit Cardiac Myocytes

    Expression of the D2 and D3dopamine Receptors in Insect Cells Using the Baculovirus System

    Production and Characterization of Soluble Mouse and Human Interferon-γ Receptors (IFN-γr) from Insect Cells

    Generation of DNA-Packaging Proteins by Overexpression in the Baculovirus/Insect Cell System

    Metalloproteinases and TIMPs Production by Aids-Kaposi Sarcoma Derived Cells

    Endothelial Cells and Their Infection with the Rickettsia Cowdria Rumination: Prospects for Vaccine Production

    Section 20. Post Translational Modifications

    Changes in the Glycosylation Pattern of Recombinant Proteins Effected by Defined Culture Conditions of BHK-21 Cells

    Glycosylation Analysis of a Human Monoclonal IgG Antibody Derived from a Human-Mouse Heterihybridoma

    Culture PH and Ammonia Affect Expression Rates and Glycosation of Recombinant Mouse Placental Lactogen Proteins by CHO Cells

    Analysis of the Product Consistency: Independant of Process Parameters, rt-PA Shows a Stable Glycosylation Pattern

    Galactosylation of Human/Mouse IGG Anti-NP Antibodies and Its Detection

    Characterization of Carbohydrate Chain on Light Chain of Human Monoclonal Antibody

    Comparison of the Posttranslational Modifications of Human Proteins Secreted from Different Recombinant Baculovirus Infected Insect Cell Lines

    Characterization of Intra- and Extracellular Proteases in Recombinant Mammalian and Hybridoma Cells

    Impact of Factors Secreted by Mammalian and Insect Cells on Human Monoclonal Antibody Integrity

    BiP - A Chaperone Involved in Antibody formation

    Section 21. Cells in Culture for Toxicology Studies

    Cell Systems for In Vitro Toxicology

    Hepatocytes: In Vitro Pharmacology, Toxicology and Drug Metabolism

    Development of an Optimized In Vitro System for Measurement of Human Tumor Response to Cytotoxic Agents

    Three Dimensional Cell Cultures for Toxicity Testing and for the Estimation of the Affinity of Cells to Biocompatible Materials

    Stable Expression of the Bovine Adrenal 11β-Hydroxylase, a Cytochrome P450 Enzyme, in CHO Cells as Tool for Drug Characterization

    Activation and Expansion of Human Cytotoxic T Lymphocytes in Hollow Fiber Bioreactors

    Section 22. Product Safety and Consistency Testing

    Significance of Contamination with Viruses of Cell Lines Used in the Production of Biological Medicinal Products

    Residual In Vitro Activity of DNA in Biologicals Treated by Beta-Propiolacetone: Rabies Vaccines as Model

    The Development of Appropriate Viral Models for the Validation of Viral Inactivation Procedures

    Regulatory Affairs: Safety Testing for Gene Therapy Products

    Product Quality Studies of Antithrombin III during Medium Recycling in Pilot Scale

    Product Consistency During Long Term Fed-Batch Culture

    The Application of Insect Cells for Biopharmaceutical Production: Implications for Safety Testing

    Generation of Linear Epitope Specific Antibodies against the Human Recombinant Tissue-Plasminogen-Activator (Rt-Pa)

    List of Participants

    Author Index


Product details

  • No. of pages: 852
  • Language: English
  • Copyright: © Butterworth-Heinemann 1994
  • Published: January 1, 1994
  • Imprint: Butterworth-Heinemann
  • eBook ISBN: 9781483162935

About the Editors

R. E. Spier

J. B. Griffiths

W. Berthold

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