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Animal Cell Technology - 1st Edition - ISBN: 9780750604215, 9781483145075

Animal Cell Technology

1st Edition

Developments, Processes and Products

Editors: R. E. Spier J. B. Griffiths C. Macdonald
eBook ISBN: 9781483145075
Imprint: Butterworth-Heinemann
Published Date: 6th July 1992
Page Count: 784
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Animal Cell Technology: Developments, Processes and Products is a compilation of scientific papers presented at the 11th European Society for Animal Cell Technology (ESACT) Meeting, held in Brighton, United Kingdom. The book is a collection of various works of scientists, engineers, and other specialists from Europe and other parts of the world who are working with animal cells. The book’s aim is to communicate experiences and research findings on the development of cell systems. The research papers are grouped into 25 sections encompassing 145 chapters. Subjects covered range from cells and physiology engineering dealing with cell characterization, cell culture establishment, cloning, and cell engineering. Topics on culture media, ammonium detoxification, the effects of physical parameters on cell cultures, assays and monitoring systems, and bioreactor techniques are also covered. Discussions are likewise made on the products from animal cells in culture, virus removal, and DNA determination and characterization in relation to safety issues. The book will be useful for cell biologists, molecular biologists, biochemists, biochemical engineers, and students engaged in the study of animal cell cultures.

Table of Contents

Section 1. Cell Characterization

The Characterization of SV40-Immortalized Murine Macrophage Cell Lines

Identification of Type-D Retroviruses as Contaminants in Widely Used Cell Lines

Characterization of Human Endothelial Hybrid Cells Expressing Von Willebrand Factor

Primary Cultures of Human Proximal Tubule Cells (HPTC) in Serum Free Defined Media: A Characterization Study F

Section 2. Cell Culture Establishment

Culture of Cells from Adult Zebrafish (Brachydanio Rerio) and Embryo

Cloning of Mouse Hybridoma Cells Infected With MYC and RAS-Containing Retroviruses Yields Cell Lines with Improved Growth and Antibody Production

Survival of B Lymphocytes Is Prolonged after Infection with Immortalizing Viruses

Establishment of Dermal Papilla Cell Lines by Retrovirus-Mediated Transfer of Oncogenes

Immortalization of Flatfish (Paralichthys Olivaceus) Leukocytes by Oncogene Transfection

Section 3. Engineering Cells for Expression

Host Cell Derived Retroviral Sequences Enhance Transfection and Expression Efficiency in CHO Cells

Stability of Production of Recombinant Antibodies from Glutamine Synthetase Amplified CHO and NSO Cell Lines

The Rapid Production of Recombinant Rabbit Metalloproteinases in Myeloma Cells

Long Term Stability of Expression of Humanized Monoclonal Antibody Campath 1-H in Chinese Hamster Ovary Cells

Oncogene-Activated Production of Recombinant Proteins for Animal Cells

Immunoglobulin Gene Expression during Hybridoma Batch and Semicontinuous Cultures

A Novel Mammalian Expression System Based on the Globin Locus Control Region

Transient Increase of Production in Fused Recombinant CHO Cells

A Comparison of Eukaryotic Control Elements Using Tissue Plasminogen Activator (tPA) as a Reporter Gene

Section 4. Physiological Modulation of Expression

Dissociation of MAB Production from Cell Division Using DNA Biosynthesis Inhibitors

The Effect of Tetramethyl Urea Α-Interferon Production by Namalwa Cells

The Effect of Sequential Induction of A-Interferon Production by Namalwa Cells

The Influence of Some Natural and Synthetical Substrata upon the Behavior of Some Cell Lines In Vitro

Differentiated Liver Function in Rat Hepatocytes Transfected with SV40 Viral DNA

Determinant Factors for Expression of Recombinant Genes in CHO Cells

New Kinetic Parameters of the Growth of Animal Cells

Section 5. Culture Media

The Preparation from Human Plasma of a Cell Medium Supplement than Can Be Pasteurized

Free Fatty Acid Utilization of Different Hybridoma Cell Lines and Its Effect on Cell Growth, Metabolism and Antibody Formation

Pluronic Adsorption Effects in Cell Culture Media

Influence of Medium Composition on the Death and Lysis of Hybridoma Cells in Continuous Cultures

Section 6. Serum Free Media

Basic Structure of a Databank - Knowledge Based System for the Computer Aided Design of Serum-Free Cell Culture Media

Long Term Serum-Free Hollow-Fiber Culture of Cell Lines Producing Human Monoclonal Antibodies: Metabolic Aspects

Serum Free Cryopreservation of Antibody Producing Cells

Growth of HeLa Cells in a Chemically Defined Medi-Cult® Medium

Adaptation of rCHO Cells to Growth in Serum Free Medium: Flow Cytometric Study

Growth, Subculture and Cryopreservation of Anchorage-Dependent Cells: A Complete Serum-Free System

Cryoprotection and Cultivation of Hybridoma Cell Lines under Serum Free Condition

Production and Characterization of a Recombinant Factor VIII Expressed by CHO Cells Cultured in Serum Supplemented or in Serum Free Media

A Defined Serum-Free Medium for Diverse Cell Culture Applications

Adaptation of a Murine Monoclonal Hybridoma Cell Line to a Low Cost Serum-Free Medium by a Repeated Batch Fermentation Procedure

Section 7. Ammonium Detoxification

Potassium Ions Affect the Ammonia/Ammonium Ion Toxicity in Animal Cell Cultivations

Development of Strategies for the Removal of Ammonia from Animal Cell Cultures

Integrated Detoxification: Reduction of Ammonium Concentration by Dialysis with Cation Exchange Membranes

Growth and Metabolism of Recombinant CHO Cell-Lines in Serum Free Medium Containing Derivatives of Glutamine

Genetic Modification of Hybridoma Glutamine Metabolism: Physiological Consequences

Growth Study of Lactate and Ammonia Double-Resistant Clones of HL-60 Cells

Section 8. The Effect of Physical Parameters on Cell Cultures

The Effect of Reduced Temperature on A-Interferon Production by Namalwa Cells

Effect of Reducing the Serum or Albumin Concentrations on the Shear Sensitivity of Two Hybridoma Cell Lines in Sparged Cultures

Mechanical Damage of Suspended Animal Cells in Directly Sparged Bioreactors

Adaption of Hybridoma Cells to Hydrodynamic Stress under Continuous Culture Conditions

Calcium Transients in SF-9 Insect Cells Exposed to Fluid Stresses Representative of Turbulent Eddies

Dissolved Oxygen - The Critical Parameter at Cell Growth and Expression of R-Protein in a Porous Microcarrier System

The Biological Effects of Dissolved Oxygen Tension on a Hybridoma Cell Line and Its Control as a Means of Improving M Ab Production in a Protein-Free Medium

The Effect of High Gas Sparge Rates in Airlift Fermenter Culture on Hybridoma Cell Growth and Antibody Production in Low Protein Medium

A Kinetic Study of Hybridoma Semicontinuous Culture: Effects of Dilution Rate

Section 9. Chemical Effects on Cell Physiology

Preliminary XH-NMR Studies of a Tumor Cell

A Simulation of Hybridoma Growth Including Sugar and Amino Acid Profiles

Carbohydrate Metabolism

Energy Metabolism of Murine Hybridomas

Complex Medium Supplements Give Difficulties When Investigating Mammalian Cell Physiology

Metabolic Quotients for Recombinant BHK Cells Grown in Repeated Batch Mode on Cytodex III Microcarriers

Hybridoma Growth and Antibody Production in Extended Stationary States

Section 10. Media Management of Cell Cultures

Metabolic Management of a Hybridoma Cell Line

Long Term Application of Medium Recycling for Economic Antibody Production

Cultivation Strategy and Bioreactor System for Optimal Medium Re-Use and Detoxification during Continuous Perfusion Processes

Applicability of Fed Batch Processes for Improving Recombinant Protein Production by the Baculovirus Expression System

Amino Acid Metabolism in Batch and Fed-Batch Culture of a Murine Myeloma Cell

Continuous Hybridoma Suspension Cultures: Changes in Feed Medium Composition and Their Effects on Extracellular and Intracellular Metabolite Concentrations

Experimental Validation of a Reaction Mechanism for a Class of Animal Cell Culture

Section 11. New Assays and Monitoring Systems

Monitoring of Biosynthetic and Metabolic Activity in Animal Cell Culture Using Flow Cytometric Methods

Monitoring and Control of Hybridoma Cell Line MN12 in a Continuous Perfusion Culture System

Development of a Novel Micromanipulation Technique for Determining Mechanical Properties of Single Mammalian Cells

Release of Cellular Enzymes for Evaluating the Dead Cell Number in Bioreactor Cultures

Quantitative Cell Adhesion Measurements on Substrates of Varying Wettability

Section 12. Suspension Cell Cultures

Productivity of a Hybridoma Cell Line in a Range of Suspension and Immobilized Culture Systems

Human Hybridoma Producing Antibodies to Pseudomonas Aeruginosa: Scale up and Optimization of IgM Production in Stirred Bioreactors

Comparison of Growth and Antibody Production of a Hybridoma Cell Line Growth in Static Flask and Suspension Culture

Section 13. Batch versus Perfusion

Perfusion vs Batch Culture: Which is the Preferred Process?

Perfusion Systems for Hybridoma Cells Based on Sedimentation in Chambers and Erlenmeyer Flasks

Aspects of Continuous Culture vs Batch Mode for Animal Cells in Industrial Processes

Perfusion Bioreactor Performance at Different Cell Bleed Rates

A Study of an Inexpensive Perfusion of a Mouse Hybridoma

A Comparative Study of Monoclonal Antibody Yield Using Batch, Continuous or Perfusion Suspension Culture Techniques

Section 14. New Bioreactor Techniques

Development of a Reactor-Integrated Aeration System for Cultivation of Animal Cells in Fluidized Beds

Oxygenation in Fluidized Bed Bioreactors Using the Microsparging Technique

Pilot Scale Modular Hard- and Software Concept for Animal Cell Fermentation

A Novel Perfusion System for the Large Scale Cultivation of Animal Cells Based on a Continuous Flow Centrifuge

Production Tissue Culture by Robots

Section 15. Cell Aggregate Cultures

Aggregate Suspension Cultures of BHK Cells

The Growth of Vero Cells as Suspended Aggregates in Serum-Free Medium

Tuning of Shear Sensitivity of CHO Cells and Its Correlation with the Size Distribution of Cell Aggregates

High Density Aggregate Culture of Recombinant CHO Cells in Fluidized Bed Bioreactors

Elimination of Cell Aggregation in Suspension Cultures of Chinese Hamster Ovary (CHO) Cells

Section 16. Spin Filter Cultures

Evaluation of a Spin Filter during Perfusion Culture of Recombinant CHO Cells

Spin Filter for Continuous Perfusion of Suspension Cells

Improved Cell Retention System Based on the Rotating Sieve Technique

Optimization of Spin-Filter Performance in the Intensive Culture of Suspended Beds

Section 17. Packed Bed Cultures

Metabolite Parameters Modulating MAB Production in a Fixed Bed, Glass Sphere Reactor

Continuous Production of Monoclonal Antibodies in Celligen Packed Bed Reactor Using Fibra-Cel Carrier

Collagenated Vesicular Plant Parenchym Material as a New Type of Surface Carrier in Animal Cell Culture

Immobilization of Animal Cells on Chemically Modified Siran Carrier

Section 18. Fluidized Bed Cultures

Introduction to Session

High Density BHK Culture Using Porous Microcarriers

The Production of Recombinant Human Interleukin-2 in a Fluidized Bed Bioreactor

Fluidized-Bed Bioreactor Perfusion Culture for the Production of Therapeutic Proteins

Development of a New Type of Macroporous Carrier

Scale-up of a Fluidized Bed Reactor Operated with Porous Glass Carriers

Efficient Serial Propagation of W138 Cells on Porous Microcarriers (Cultispher GL)

Production of T-PA by C-127 Cells Cultivated on Different Porous Microcarriers in a Perfused Fluidized Bed Reactor

Section 19. Hollow Fiber Cultures

Scaling up of a Hollow Fiber Reactor for Animal Cell Cultivation

Production of Concentrated Monoclonal Antibody Containing Supernatent in Microporous Ceramic Tubes

Scale up of Human IgGl Production by a Lymphoblastoid Cell Line in Hollow Fiber Systems

Long Term Production of Soluble CD23 in Hollow Fiber Reactors

Simultaneous Cultivation of Two Hybridoma Cell Lines in A Hollow Fiber Bioreactor

A Dual Fiber Membrane Reactor for the Cultivation of Mammalian Cells

Section 20. Secreted Proteases

Production of Collagenase by Anchorage Dependent Cells in Culture

Activation of a Specific Proteolytic Activity in Suspension Cultures of Recombinant Adherent Cells

Section 21. Products from Animal Cells in Culture

Dependence of the Pharmacokinetic Properties of Monoclonal IgM on Its n-Glycosylation

Production of Recombinant Soluble Human TNF Receptor Using the Baculovirus-Insect Cell Expression System

Expression of Recombinant Proteins in High Density Insect Cell Cultures

Production of Recombinant Epidermal Growth Factor Receptor Tyrosine Kinase (EGFR-TK) Domain in an Insect Cell/Baculovirus Expression System

Recombinant Protein Production by a Murine Plasmacytoma

Methods Useful for the Generation of Bispecific Antibodies

Production of Human Gamm-Glutamyltransferase by Mass Cultures of Human Hepatoma and Recombinant CHO Cells

Production of Recombinant Rubella Antigens

Novel Plasminogen Activators from the Vampire Bat Desmondus Rotundus

Immunoglobulin Fragments during Fermentation of a Human Monoclonal Anti-Erythrocyte Antibody

Section 22. Culture Supernatent Purification and Virus Removal

Microporous Membrane Ion Exchanger for Rapid Purification of Monoclonal Antibodies

Purification of Monoclonal Antibody from Culture Supernatent Produced in Hollow Fiber Bioreactors

Virus Removal-Inactivation in Downstream Processing

A Unique and Validatable Membrane-Based System Capable of High Resolution Removal of Viruses from Protein Solutions

Retention of Viral Contaminants by Microfiltration

Section 23. Validation of Virus Removal Processes

Retroviruses: Regulatory Issues for Biological Products and Virus Vectors

Detection of Viruses in Recombinant Cells by Electron Microscopy

The Detection of Human Viruses in Cell Lines

New Cell Lines for Virus Diagnosis

The Development of Appropriate Virus Models for Validating Therapeutic Protein Purification Processes

Product Process Validation with Human Immunodeficiency Virus-I

Validation of Processes for the Removal and/or Inactivation of These Agents

Section 24. DNA Determination and Characterization in Relation to Safety Issues

Validation of DNA Fingerprinting in Animal Cell Technology: The Differentiation and Identification of Murine Hybridoma Clones for a Single Fusion and the Stability of Transformed Cell Lines

Molecular Hybridization Techniques and Polymerase Chain Reaction (PCR) as Methods for Safety Assessment of Animal Cells Used in Biopharmaceutical Production

Quantitation of Residual DNA in Biological Products: New Regulatory Concerns and New Methodologies

Section 25. Using Cell Cultures for In-Vitro Toxicity Tests

The Development and Validation of In-Vitro Toxicity Tests

Renal Epithelial Cells in Culture: A Comparative Study of the Functional Activities of Rabbit Kidney Proximal Tubule Cells in Primary Cultures and the LLC-PK1 and OK Cells

Rabbit Kidney Proximal Tubule Cells in Primary Culture as an In-Vitro Model for Evaluating Platinum-Derivate-Induced Nephrotoxicity

Sertoli Cell Culture Model for Assessing Testicular Toxicity

Can A Toxicity Test Be Discriminant for Target Organ Toxicity?

List of Delegates

Subject Index

Author Index


No. of pages:
© Butterworth-Heinemann 1992
6th July 1992
eBook ISBN:

About the Editors

R. E. Spier

J. B. Griffiths

C. Macdonald

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