Animal Cell Culture Methods - 1st Edition - ISBN: 9780125441599, 9780080859552

Animal Cell Culture Methods, Volume 57

1st Edition

Serial Editors: Leslie Wilson Paul Matsudaira
Serial Volume Editors: Jennie Mather David Barnes
eBook ISBN: 9780080859552
Imprint: Academic Press
Published Date: 12th June 1998
Page Count: 368
Sales tax will be calculated at check-out Price includes VAT/GST
175.00
122.50
122.50
122.50
140.00
122.50
122.50
140.00
161.00
112.70
112.70
112.70
128.80
112.70
112.70
128.80
105.00
73.50
73.50
73.50
84.00
73.50
73.50
84.00
131.00
91.70
91.70
91.70
104.80
91.70
91.70
104.80
Unavailable
Price includes VAT/GST

Institutional Subscription

Secure Checkout

Personal information is secured with SSL technology.

Free Shipping

Free global shipping
No minimum order.

Table of Contents

Principles of Cell Culture:

D. Barnes, Animal Cell Culture Equipment and Techniques.

J.P. Mather, Making Informed Choices: Medium, Serum, and Serum-free Medium. How to Choose the Appropriate Medium and Culture System for the Model You Wish to Create.

R.J. Hay, Cell Line Availability: Where to Get the Cell Lines You Need.

C.K. Lincoln and M.G. Gabridge, Cell Culture Contamination: Sources, Consequences, Prevention, and Elimination.

Establishing Cell Lines:

Y. Katakura, S. Alam, and S. Shirahata, Immortalization by Gene Transfection.

M.-C. Hofmann and J.L. Millan, Establishment of Mammalian Testicular Cell Lines.

S. Shirahata, Y. Katakura, and K. Teruya, Cell Hybridization, Hybridomas, and Human Hybridomas.

M. Westphal and H. Meissner, Establishing Human Glioma-Derived Cell Lines.

R. Li, Culture Methods for Selective Growth of Normal Rat and Human Schwann Cells.

C.J. Bayne, Invertebrate Cell Culture Considerations: Insects, Ticks, Shellfish, and Worms.

J. Kaplan and B. Hukku, Cell Line Characterization and Authentication.

Specialized Culture Techniques:

J.P. Mather, Laboratory Scale-up of Cell Cultures (0.5-50 Liters).

G.F. Merrill, Cell Synchronization.

D.T. Loo and J.R. Tillema, Measurement of Cell Death.

A. Moore, C.J. Donahue, K.D. Bauer, and J.P. Mather, Simultaneous Measurement of Cell Cycle and Apoptotic Cell Death.

M. Pirity, A.-K. Hadjantonakis, and A. Nagy,Embryonic Stem Cells, Creating Transgenic Animals.

Microscopy and Morphology:

D.M. Phillips, Electron Microscopy: Use of Transmission and Scanning Electron Microscopy to Study Cells in Culture.

S.P. Wheatly and Y.-L. Wang, Indirect Immunofluorescence Microscopy in Cultured Cells.

T.K. Woodruff, Cellular Localization of mRNA and Protein: In Situ Hybridization Histochemistry and In Situ Ligand Binding. Subject Index.


Description

This volume provides complete and thorough coverage of the classical and state-of-the-art methods used in cell culture. It also includes basic principles used in the selection of cells for specific scientific study, as well as analytical and procedural techniques.

Key Features

@introbul:Key Features @bul:* Reviews basic principles of cell culture

  • Gives options and techniques on how to look at cells

Readership

Graduate students in basic research, scientists, technicians, postdoctoral, and experienced researchers in fields of cell and developmental biology, genetics, and biochemistry.


Details

No. of pages:
368
Language:
English
Copyright:
© Academic Press 1998
Published:
Imprint:
Academic Press
eBook ISBN:
9780080859552

Ratings and Reviews


About the Serial Editors

Leslie Wilson Serial Editor

Affiliations and Expertise

University of California, Santa Barbara, USA

Paul Matsudaira Serial Editor

Affiliations and Expertise

Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, U.S.A.

About the Serial Volume Editors

Jennie Mather Serial Volume Editor

Affiliations and Expertise

Founder, President and CSO, Raven biotechnologies, Inc., South San Francisco, CA, USA

David Barnes Serial Volume Editor

Affiliations and Expertise

Oregon State University, Corvallis, U.S.A.