Animal Cell Culture Methods

Animal Cell Culture Methods

1st Edition - June 12, 1998
  • Editors: Leslie Wilson, Paul Matsudaira
  • eBook ISBN: 9780080859552

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This volume provides complete and thorough coverage of the classical and state-of-the-art methods used in cell culture. It also includes basic principles used in the selection of cells for specific scientific study, as well as analytical and procedural techniques.

Key Features

@introbul:Key Features
@bul:* Reviews basic principles of cell culture
* Gives options and techniques on how to look at cells


Graduate students in basic research, scientists, technicians, postdoctoral, and experienced researchers in fields of cell and developmental biology, genetics, and biochemistry.

Table of Contents

  • Principles of Cell Culture:
    D. Barnes, Animal Cell Culture Equipment and Techniques.
    J.P. Mather, Making Informed Choices: Medium, Serum, and Serum-free Medium. How to Choose the Appropriate Medium and Culture System for the Model You Wish to Create.
    R.J. Hay, Cell Line Availability: Where to Get the Cell Lines You Need.
    C.K. Lincoln and M.G. Gabridge, Cell Culture Contamination: Sources, Consequences, Prevention, and Elimination.
    Establishing Cell Lines:
    Y. Katakura, S. Alam, and S. Shirahata, Immortalization by Gene Transfection.
    M.-C. Hofmann and J.L. Millan, Establishment of Mammalian Testicular Cell Lines.
    S. Shirahata, Y. Katakura, and K. Teruya, Cell Hybridization, Hybridomas, and Human Hybridomas.
    M. Westphal and H. Meissner, Establishing Human Glioma-Derived Cell Lines.
    R. Li, Culture Methods for Selective Growth of Normal Rat and Human Schwann Cells.
    C.J. Bayne, Invertebrate Cell Culture Considerations: Insects, Ticks, Shellfish, and Worms.
    J. Kaplan and B. Hukku, Cell Line Characterization and Authentication.
    Specialized Culture Techniques:
    J.P. Mather, Laboratory Scale-up of Cell Cultures (0.5-50 Liters).
    G.F. Merrill, Cell Synchronization.
    D.T. Loo and J.R. Tillema, Measurement of Cell Death.
    A. Moore, C.J. Donahue, K.D. Bauer, and J.P. Mather, Simultaneous Measurement of Cell Cycle and Apoptotic Cell Death.
    M. Pirity, A.-K. Hadjantonakis, and A. Nagy,Embryonic Stem Cells, Creating Transgenic Animals.
    Microscopy and Morphology:
    D.M. Phillips, Electron Microscopy: Use of Transmission and Scanning Electron Microscopy to Study Cells in Culture.
    S.P. Wheatly and Y.-L. Wang, Indirect Immunofluorescence Microscopy in Cultured Cells.
    T.K. Woodruff, Cellular Localization of mRNA and Protein: In Situ Hybridization Histochemistry and In Situ Ligand Binding.
    Subject Index.

Product details

  • No. of pages: 368
  • Language: English
  • Copyright: © Academic Press 1998
  • Published: June 12, 1998
  • Imprint: Academic Press
  • eBook ISBN: 9780080859552

About the Serial Editors

Leslie Wilson

University of California, Santa Barbara, USA

Affiliations and Expertise

University of California, Santa Barbara, USA

Paul Matsudaira

Affiliations and Expertise

Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, U.S.A.

About the Serial Volume Editors

Jennie Mather

Affiliations and Expertise

Founder, President and CSO, Raven biotechnologies, Inc., South San Francisco, CA, USA

David Barnes

Affiliations and Expertise

Oregon State University, Corvallis, U.S.A.