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Studying cell signaling pathways; Flow mediated signaling; Endothelial cell adhesion and migration on the ECM; EC sprouting assays; Lumen formation assays; Isolation and propagation of EC's or SMC's; Cell-based assays to screen anti-angiogenic agents (chemical approach; ES cell models of vascular network formation; Co-culture studies with EC plus other cell types; Aortic ring sprouting (multiple cell types present); Effects of hypoxia or hyperoxia; Vascular Proteomics; In vitro vascular barrier function; EC leukocyte interactions; ECM signaling to the vasculature; Vascular Integrin signaling; Mechanical Signaling in blood vessels; Vascular and neuronal patterning; Vascular growth factors and their signals; VEGF receptor signal transduction; Development and testing of monoclonal antibodies directed to angiogenic targets.
Angiogenesis is the growth of new blood vessels and is an important natural process in the body. A healthy body maintains a perfect balance of angiogenesis modulators. In many serious disease states, however, the body loses control over antiogenesis. Diseases that are angiogensis-dependent result when blood vessels either grow excessively or insufficiently.
Tried-and-tested techniques written by researchers that developed them, used them, and brought them to fruition
Provides the "builder's manual" for essential techniques--a one-stop shop that eliminates needless searching among untested techniques
Includes step-by-step methods for understanding the cell and molecular basis of wound healing, vascular integrin signaling, mechanical signaling in blood vessels, and vascular proteomics
Researchers in biochemistry, cell and molecular biology, physiology, neurology, oncology, pathology, cardiology, rheumatology, opthamology, and genetics
- No. of pages:
- © Academic Press 2008
- 2nd September 2008
- Academic Press
- Hardcover ISBN:
- eBook ISBN:
The Scripps Research Institute, La Jolla, CA, USA
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