Analytical Techniques in Biosciences

Analytical Techniques in Biosciences

From Basics to Applications

1st Edition - October 21, 2021

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  • Editors: Chukwuebuka Egbuna, Kingsley Patrick-Iwuanyanwu, Muhammad Ajmal Shah, Jonathan C. Ifemeje, Azhar Rasul
  • eBook ISBN: 9780128227992

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Description

Analytical Techniques in Biosciences: From Basics to Applications presents comprehensive and up-to-date information on the various analytical techniques obtainable in bioscience research laboratories across the world. This book contains chapters that discuss the basic bioanalytical protocols and sample preparation guidelines. Commonly encountered analytical techniques, their working principles, and applications were presented. Techniques, considered in this book, include centrifugation techniques, electrophoretic techniques, chromatography, titrimetry, spectrometry, and hyphenated techniques. Subsequent chapters emphasize molecular weight determination and electroanalytical techniques, biosensors, and enzyme assay protocols. Other chapters detail microbial techniques, statistical methods, computational modeling, and immunology and immunochemistry.The book draws from experts from key institutions around the globe, who have simplified the chapters in a way that will be useful to early-stage researchers as well as advanced scientists. It is also carefully structured and integrated sequentially to aid flow, consistency, and continuity. This is a must-have reference for graduate students and researchers in the field of biosciences.

Key Features

  • Presents basic analytical protocols and sample-preparation guidelines
  • Details the various analytical techniques, including centrifugation, spectrometry, chromatography, and titrimetry
  • Describes advanced techniques such as hyphenated techniques, electroanalytical techniques, and the application of biosensors in biomedical research
  • Presents biostatistical tools and methods and basic computational models in biosciences

Readership

Students and researchers across the biosciences

Table of Contents

  • Cover image
  • Title page
  • Table of Contents
  • Copyright
  • List of contributors
  • About the editors
  • Chapter 1. Bioanalysis: methods, techniques, and applications
  • Abstract
  • Abbreviations
  • 1.1 Introduction
  • 1.2 Bioanalytical methods
  • 1.3 Sample preparation
  • 1.4 Bioanalytical techniques
  • 1.5 Method development
  • 1.6 Bioanalytical method validation
  • 1.7 Analytical challenges
  • 1.8 Applications
  • 1.9 Conclusion
  • References
  • Chapter 2. Sample preparation techniques in biological and pharmaceutical sciences
  • Abstract
  • Abbreviations
  • 2.1 Introduction
  • 2.2 Biological samples description
  • 2.3 Basic notions regarding biological samples preparation
  • 2.4 Benefits of sample preparation
  • 2.5 Conventional extraction techniques
  • 2.6 Techniques based on microextraction technologies
  • 2.7 Common mistakes during sample preparation
  • 2.8 Conclusion and perspectives
  • References
  • Chapter 3. Centrifugation techniques
  • Abstract
  • Abbreviations
  • 3.1 Introduction
  • 3.2 Principles of centrifugation
  • 3.3 Types of centrifugation
  • 3.4 Centrifuge
  • 3.5 Maintenance and care of centrifugation equipments
  • 3.6 Applications of centrifugation
  • 3.7 Conclusion
  • References
  • Chapter 4. Electrophoretic techniques
  • Abstract
  • 4.1 Introduction
  • 4.2 Electrophoresis
  • 4.3 Gel electrophoresis
  • 4.4 Agarose gel electrophoresis
  • 4.5 Polyacrylamide gel electrophoresis
  • 4.6 Staining procedures
  • 4.7 Isoelectric focusing
  • 4.8 Immobilized pH gradients
  • 4.9 Proteome analysis
  • 4.10 Functional proteomics
  • 4.11 Gradient gel
  • 4.12 Two-dimensional electrophoresis
  • 4.13 Two-dimensional differential gel electrophoresis
  • 4.14 Conclusion and future direction
  • References
  • Chapter 5. Chromatographic techniques: types, principles, and applications
  • Abstract
  • Abbreviations
  • 5.1 Introduction
  • 5.2 Chromatographic techniques based on the bed shape
  • 5.3 Chromatographic techniques based on the physical state of mobile phase
  • 5.4 Chromatographic techniques based on the separation mechanism
  • 5.5 Specialized techniques of chromatography
  • 5.6 Future perspectives
  • 5.7 Conclusion
  • References
  • Chapter 6. Titrimetry
  • Abstract
  • 6.1 Introduction
  • 6.2 Instrumentation in titrimetry
  • 6.3 Categorization of titrimetric methods
  • 6.4 Challenges associated with titrimetry
  • 6.5 Modern application of titrimetry
  • 6.6 Conclusion
  • References
  • Chapter 7. Mass spectrometry
  • Abstract
  • 7.1 Introduction
  • 7.2 Instrumentation
  • 7.3 Ionization processes
  • 7.4 Application
  • 7.5 Conclusion
  • References
  • Chapter 8. Hyphenated techniques
  • Abstract
  • Abbreviations
  • 8.1 Introduction
  • 8.2 Gas chromatography tandem mass spectroscopy
  • 8.3 Liquid chromatography tandem mass spectroscopy
  • 8.4 Liquid chromatography tandem nuclear magnetic resonance
  • 8.5 Liquid chromatography tandem infrared spectroscopy
  • 8.6 Gas chromatography tandem infrared spectroscopy
  • 8.7 Capillary electrophoresis tandem mass spectrometry
  • 8.8 Differential scanning calorimetry tandem Raman spectroscopy
  • 8.9 Thermogravimetry tandem GC-MS
  • 8.10 Conclusion and future prospects
  • References
  • Chapter 9. Molecular weight determination of nucleic acids and proteins
  • Abstract
  • 9.1 Introduction
  • 9.2 Molecular weight determination of nucleic acid
  • 9.3 Molecular weight determination of protein
  • 9.4 Conclusion
  • References
  • Chapter 10. Electroanalytical techniques in biosciences: conductometry, coulometry, voltammetry, and electrochemical sensors
  • Abstract
  • 10.1 Introduction
  • 10.2 Electrochemical cell and cell potential measurements
  • 10.3 Reference electrodes and electrochemical measurements
  • 10.4 Conductometry
  • 10.5 Coulometry
  • 10.6 Voltametric techniques
  • 10.7 Microelectrodes
  • 10.8 Chemically modified electrodes and electrochemical sensors
  • 10.9 Conclusion
  • 10.11 Electrochemistry general questions
  • References
  • Chapter 11. Biosensors: components, mechanisms, and applications
  • Abstract
  • 11.1 Introduction
  • 11.2 Biosensors
  • 11.3 History of biosensors
  • 11.4 Biocomponents
  • 11.5 Characteristics of biosensors
  • 11.6 The working mechanism of biosensors
  • 11.7 Designing of biosensor
  • 11.8 Three generations of biosensors
  • 11.9 Bioreceptor molecules and biosensing techniques
  • 11.10 Clinical application areas of biosensors
  • 11.11 Conclusion and prospects
  • References
  • Chapter 12. Enzyme assay techniques and protocols
  • Abstract
  • 12.1 Introduction
  • 12.2 Enzymatic assays in drug discovery
  • 12.3 Enzymatic assays as diagnostic tools for diseases
  • 12.4 Conclusions and future recommendation
  • References
  • Chapter 13. Microbial techniques and methods: basic techniques and microscopy
  • Abstract
  • Abbreviations
  • 13.1 Introduction
  • 13.2 Culturing microorganisms; preparation and sterilization of microbiological media
  • 13.3 Determination of microbial numbers
  • 13.4 Pure culture techniques
  • 13.5 Stains and staining techniques
  • 13.6 Identification of microorganisms
  • 13.7 Antimicrobial susceptibility testing
  • 13.8 Microscopy
  • 13.9 Conclusion
  • References
  • Chapter 14. Computational modeling and tools in biosciences: bioinformatics approach
  • Abstract
  • 14.1 Introduction
  • 14.2 Enabling biological discovery with computational modeling and simulation
  • 14.3 Computational tools
  • 14.4 Conclusion
  • References
  • Chapter 15. Statistical methods and tools in biosciences
  • Abstract
  • 15.1 Introduction
  • 15.2 Preliminary statistical methods
  • 15.3 Methods of data analysis
  • 15.4 Conclusion
  • 15.5 Sample exercises
  • References
  • Further reading
  • Chapter 16. Immunology and immunochemistry
  • Abstract
  • 16.1 Introduction
  • 16.2 Immunochemistry
  • 16.3 Immunocytochemistry
  • 16.4 Applications of immunochemistry
  • 16.5 Conclusion
  • References
  • Index

Product details

  • No. of pages: 300
  • Language: English
  • Copyright: © Academic Press 2021
  • Published: October 21, 2021
  • Imprint: Academic Press
  • eBook ISBN: 9780128227992

About the Editors

Chukwuebuka Egbuna

Chukwuebuka Egbuna is a chartered chemist, a chemical analyst, and an academic researcher. He is a member of the Institute of Chartered Chemists of Nigeria (ICCON), the Nigerian Society of Biochemistry and Molecular Biology (NSBMB), the Society of Quality Assurance (SQA) (USA), and the Royal Society of Chemistry (RSC) (United Kingdom). Egbuna is also an editor of new Elsevier series on Drug Discovery Update. The series includes books, monographs and edited collections from all areas of drug discovery, including emerging therapeutic claims for the treatment of diseases. He has been engaged in a number of roles at New Divine Favor Pharmaceutical Industry Limited, Akuzor Nkpor, Anambra State, Nigeria, and Chukwuemeka Odumegwu Ojukwu University (COOU) in Nigeria. He has collaboratively worked and published quite a number of research articles in the area of phytochemistry, nutrition and toxicology and related areas. He is a reviewer and editorial board member of various journals, including serving as a website administrator for the Tropical Journal of Applied Natural Sciences (TJANS), a journal of the faculty of Natural Sciences, COOU and as an advisory board member under toxicology for the book publisher, Cambridge Scholar Publishing. His primary research interests are in phytochemistry, nutrition and toxicology, food and medicinal chemistry, and analytical biochemistry.

Affiliations and Expertise

Nutritional Biochemistry Unit, Africa Centre of Excellence in Public Health and Toxicological Research (ACE-PUTOR), University of Port-Harcourt, Rivers State; Department of Biochemistry, Chukwuemeka Odumegwu Ojukwu University, Uli, Nigeria

Kingsley Patrick-Iwuanyanwu

Dr. Kingsley Chukwuemeka Patrick-Iwuanyanwu is a Senior Lecturer in the Department of Biochemistry. Dr. Patrick-Iwuanyanwu is a recipient of The World Academy of Science- International Centre for Chemical and Biological Sciences (TWAS-ICCBS) Postgraduate fellowship award in 2009 to University of Karachi, Pakistan. He was among the 100 young scientists from around the world selected to attend The World Life Sciences Forum by BioVision (Lyon, France); He is a recipient of Society of Toxicology (SOT) /AstraZenecca IUTOX fellowship award in 2009. He served as a pioneer African representative and one of the founding Executive Members of Student Advisory Council (SAC) of Society of Environmental Toxicology and Chemistry (SETAC, EUROPE) from 2009-2011. Dr. Patrick-Iwuanyanwu is a member of several professional bodies including Nigerian Society of Biochemistry and Molecular Biology (NSBMB); Africa Education Initiative (NEF, CT. USA); West African Society of Toxicology (WASOT); Society of Environmental Toxicology and Chemistry (SETAC, Europe); Society of Toxicology (SOT, Reston, USA); Society for Experimental Biology (SEB, UK).

Affiliations and Expertise

Africa Centre of Excellence, Centre for Public Health and Toxicological Research (ACE-PUTOR), University of Port-Harcourt, Rivers State, Nigeria; Department of Biochemistry, University of Port Harcourt, Nigeria

Muhammad Ajmal Shah

Dr. Muhammad Ajmal Shah is a registered pharmacist and an academic researcher. He obtained his PharmD and MPhil in Pharmacognosy degrees from Federal Urdu University of Arts, Science & Technology, Pakistan. He obtained his PhD in Pharmaceutical Sciences from Prince of Songkla University Thailand by securing Thailand Education Hub for ASEAN Countries PhD Scholarship Award (TEH-AC 2014-17). He has published over 60 articles in well reputed international journals and has authored over 20 chapters in different books published by Elsevier and Springer. He is a reviewer and editorial board member of various reputed international journals. Dr. Shah is an active member of the American Society of Pharmacognosy, Phytochemical Society of Europe, American Society of Microbiology and GA Society for Medicinal Plant and Natural Product Research Germany. Currently, he is working as an Assistant Professor and Incharge at Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Pakistan. His primary research interests include green extraction and enrichment of bioactive compounds, their isolation and bioevaluation in metabolic and aging associated diseases.

Affiliations and Expertise

Assistant Professor, Department of Pharmacognosy, Faculty of Pharmaceutical Sciences, Government College University Faisalabad, Pakistan

Jonathan C. Ifemeje

Dr. Jonathan Chinenye Ifemeje, PhD, is an Associate Professor (Reader) in the Department of Biochemistry, Faculty of Natural Sciences, Chukwuemeka Odumegwu Ojukwu University, Nigeria. He has published over 40 articles with international publishers and has attended series of workshops and conferences. He has also served as an external examiner for various institutions. He is the managing editor of the Tropical Journal of Applied Natural Sciences and a reviewer and an editorial board member for various reputable journals. He has worked extensively in the areas of phytochemistry, environment, and nutritional toxicology. He is a member of various institutes, including the Institute of Chartered Chemists of Nigeria, the Nigerian Society of Biochemistry and Molecular Biology, and Society of Quality Assurance. Dr. Ifemeje is presently the Director of Student Industrial Work Experience Scheme (SIWES), COOU and the Department of Biochemistry Postgraduate Coordinator, COOU. He has also served as the department and faculty staff adviser. He obtained his PhD in applied biochemistry from Nnamdi Azikiwe University, Awka, Nigeria, and his MSc degree in nutrition and toxicology from the University of Port-Harcourt, Nigeria.

Affiliations and Expertise

Associate Professor, Department of Biochemistry, Faculty of Natural Sciences, Chukwuemeka Odumegwu Ojukwu University, Nigeria

Azhar Rasul

Dr. Azhar Rasul is an Assistant Professor at Faculty of Life Sciences, Government College University. He has published over 79 peer-reviewed articles and 4 book chapters. Dr. Rasul has presented several invited talks at National and International level and has attended more than 20 International conferences in Japan, China, South Korea, Dubai, Sri Lanka, Qatar, Turkey, Thailand, Malaysia and France. He has obtained several national and international research grants (HEC-Pak Turk Mobility Grant, ISSESCO RG, HEC-NRPU RG(s), The Nagai Foundation Tokyo RG(s) and COMSTECH-TWAS RG).

Affiliations and Expertise

Assistant Professor, Faculty of Life Sciences, Government College University, Faisalabad, Pakistan

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