Description

Gas chromatography remains the world's most widely used analytical technique, yet the expertise of a large proportion of chromatographers lies in other fields. Many users have little real knowledge of the variablesin the chromatographic process, the interaction between those variables, how they are best controlled, how the quality of their analytical results could be improved, and how analysis times can be shortened to facilitate the generation of a greater numberof more reliable results on the same equipment. An analyst with a more comprehensive understanding of chromatographic principles and practice, however, can often improve the quality of the data generated, reduce the analytical time, and forestall the needto purchase an additional chromatograph or another mass spectrometer.The Second Edition of Analytical Gas Chromatography is extensively revised with selected areas expanded and many new explanations and figures. The section on sample injection has been updated to include newer concepts of split, splitless, hot and cold on-column, programmed temperature vaporization, and large volume injections. Coverage of stationary phases now includes discussion, applications, and rationale of the increased thermal and oxidative resistance of the newly designed silarylenepolysiloxane polymers. Conventional and"extended range"polyethylene glycol stationary phases are examined from the viewpoints of temperature range and retention index reliabilities, and the chapter on"Variables"has been completely rewritten. The ways in which carrier gas velocity influences chromatographic performance is considered in detail, and includes what may be the first rational explanation of the seemingly anomalous effects that temperature exercises on gas viscosity (and gas flow). The practical effects that these changes cause to the chromatography is examined in pressure-, flow-, and"EPC-"regulated systems."Column Selection, Installation, and Use"has been completely rewritten a

Key Features

@introbul:Key Features @bul:* Incorporates nearly 60% new material * Covers the newest concepts and materials for sample injection and stationary phases * Presents detailed consideration of the influence of carrier gas velocity on practical aspects of chromatographic performance * Contains a chapter on "Special Analytical Techniques" which includes consideration of selectivity tuning and fast analysis * Provides a new section addressing the special requirements of columns to be used in mass spectral analysis * Includes an improved program that greatly enhances the accuracy of the Van Deemter plots by more accurately depicting localized chromatographic conditions at each point in the column

Readership

Analytical Chemists; Biochemists; Technicians.

Table of Contents

Preface. Introduction: General Considerations. A Simplistic Approach. A Simplistic Approach. Simplistic Comparisons of Packed and Open Tubular Columns. A Simplified Theory of the Chromatographic Process. Separation of Components. Effect of Carrier Gas Velocity. References. The Open Tubular Column: General Considerations. The Tubing. Sources of Activity. Structural Flaws. Flexible Columns of Conventional Glasses. Silanol Deactivation. Column Coating. References. Sample Injection: General Considerations. Extra-Chromatographic Phenomena Influencing Band Length. Chromatographic Phenomena Influencing Band Length. Hot Vaporizing Injection Methods. Programmed Temperature Vaporizing Injector (PTV). On-Column Injection. Large Volume Injection. Purge and Trap Sampling. Selecting the Proper Injection Mode. References. The Stationary Phase: General Consideration. Stationary Phase Polarity and Selectivity. Polysiloxane Stationary Phases. Aryl Substituted Siloxanes.Bonded, Crosslinked, and/or Immobilized Stationary Phases. Polyethylene Glycol Stationary Phases. Enantiomer Separations. Other Special-Selectivity Stationary Phases. Gas-Solid Absorption Columns. References. Variables in the Gas Chromatographic Process: General Considerations. Volumetric Column Flow. Carrier Gas Viscosity. Comparing Calculated to Experimental Volumetric Flows. Volumetric Column Flow & Average Linear Velocity. Regulation of Gas Flow and Gas Velocity. Average Linear Velocity & Chromatographic Efficiency. Calculating Reliable Estimates A, B, and C. Theory & Practice. Choice of Carrier Gas. The Effect of Solute Retention Factors. The Effect of Column Length. The Effect of Column I.D. The Effect of Stationary Phase Film Thickness. The Effect of Stationary Phase Diffusivity. The Effects of Temperature. Optimum Practical Gas Velocity. Temperature Programmed Considerations. Column Flow Under Temperature Programmed Conditions.

Details

No. of pages:
389
Language:
English
Copyright:
© 1997
Published:
Imprint:
Academic Press
Print ISBN:
9780123843579
Electronic ISBN:
9780080527208

Reviews

@qu:"Eminently readable...It is the sort of reference book that should be on the shelf of every laboratory that contains a gas chromatograph." @source:--Brian Bush, Wadsworth Laboratory, New York State Department in ANALYTICAL CHEMISTRY @qu:"The treatment is superb and complete. The authors style of writing makes the book easy to read and interesting. Thus, if you desire a well-written, interesting treatment on the practical considerations involved in gas chromatography, emphasizing the selection, installation, evaluation, application, and basis for the use of open tubular glass capillary columns, this book should be your choice." @source:--Peter F. Lott, University of Missouri-Kansas City in THE JOURNAL OF AMERICAN CHEMICAL SOCIETY