Analytical Applications of Immobilized Enzymes and Cells

Analytical Applications of Immobilized Enzymes and Cells

Applied Biochemistry and Bioengineering, Vol. 3

1st Edition - December 28, 1981

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  • Editors: Lemuel B. Wingard, Ephraim Katchalski-Katzir, Leon Goldstein
  • eBook ISBN: 9781483215853

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Analytical Applications of Immobilized Enzymes and Cells, Volume 3 presents a survey of the interdisciplinary interactions, developments, and trends on research in immobilized enzymes and cells. This volume is comprised of eight chapters. Chapters 1 to 4 are devoted to the description of methodologies and instrumentations which are used in routine analysis. Among those described are enzyme tubes, enzyme thermistors, and enzymic or microbial electrodes. Chapter 4 provides a theoretical analysis of electrode design. Subsequent chapters focus on solid-phase enzyme immunoassays and on techniques for in vivo monitoring of metabolites; economic evaluation of the use of high-purity enzymes in analysis; and an assessment of the significance of microprocessors and computer science in enzyme based analysis. Chemists and chemical engineers will find the book very useful.

Table of Contents

  • List of Contributors


    Contents of Previous Volumes

    Open Tubular Heterogeneous Enzyme Reactors in Continuous-Flow Analysis

    I. Introduction

    II. Continuous-Flow Analysis

    III. Enzymes in Continuous-Flow Analysis

    IV. Heterogeneous Enzyme Kinetics

    V. Theory of Open Tubular Heterogeneous Enzyme Reactors (OTHERs) with Segmented Flow

    VI. Preparation and Properties of OTHERs

    VII. Factors Affecting Performance of OTHERs

    VIII. Other Uses of OTHERs

    IX. Conclusions

    Appendix I: Procedures for the Preparation of Chemically Modified Nylon Tubes

    Appendix II: Examples of Analytical Applications of OTHERs

    List of Symbols


    Enzyme Thermistor Devices and Their Analytical Applications

    I. Introduction

    II. Experimental

    III. Applications

    IV. Comments on the Technique

    V. Concluding Remarks


    Bioelectrochemical Sensors Based on Immobilized Enzymes, Whole Cells, and Proteins

    I. Introduction

    II. Bioelectrochemical Sensors Based on Immobilized Enzymes

    III. Bioelectrochemical Sensors Based on Immobilized Whole Cells

    IV. Bioelectrochemical Sensors Based on Immobilized Proteins


    Theoretical Aspects of Enzyme Electrode Design

    I. Introduction

    II. Background

    III. Classification and Physical Description

    IV. Formulation of the Models

    V. Conclusions



    Solid Phase Enzyme Immunoassays

    I. Introduction

    II. Quantitation Using Solid Phase Enzyme Immunoassays

    III. Reagents Used In Solid Phase Enzyme Immunoassays

    IV. Concluding Comments

    Appendix: Enzyme-immunoassay Procedures


    In Vivo Monitoring of Metabolites Using Enzyme-Based Analytical Techniques

    I. Introduction

    II. Development of an in Vivo Monitoring System

    III. Future Development

    IV. Conclusions


    Economic Aspects of High-Purity Enzymes as Related to Analysis

    I. Introduction

    II. The Scope of Enzymatic Analysis

    III. Costs of Enzyme Purification

    IV. The Cost Structure of Organization in the Clinical Laboratory

    V. The Free Soluble and Immobilized Enzyme from the Standpoint of Methodology

    VI. Conclusions


    Potential Impacts of Physics and Electronics on Enzyme-Based Analysis

    I. Introduction

    II. General Aspects of Transducers

    III. Microprocessors

    IV. Microfabrication Technology

    V. Examples of Classes of Transducers of Relevance

    VI. Conclusion



Product details

  • No. of pages: 328
  • Language: English
  • Copyright: © Academic Press 1981
  • Published: December 28, 1981
  • Imprint: Academic Press
  • eBook ISBN: 9781483215853

About the Editors

Lemuel B. Wingard

Ephraim Katchalski-Katzir

Leon Goldstein

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