This volume includes a core of methodologies to attack the unique experimental problems presented by protein misassembly. Emphasis is on human biology applications, the area in which there is the most interest, in which most of the work has already been done, and in which there is the best evidence for the structural sophisitication of the protein aggregates. The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences.


Biochemists, molecular biologists, biomedical researchers, cell biologists, developmental biologists, and neuroscientists.

Table of Contents

Characterization of In Vivo Protein Deposition: A. Identification and Isolation of Agreggates: G.T. Westermark, K.H. Johnson, and P. Westermark, Staining Methods for Identification of Amyloid in Tissue. G.A. Tennent, Isolation and Characterization of Amyloid Fibrils from Tissue. G. Georgiou and P. Valax, Isolating Inclusion Bodies from Bacteria. A.E. Roher and Y.-M. Kuo, Isolation of Amyloid Deposits from Brain. B. Isolation and Characterization of Protein Deposit Components: B. Kaplan, R. Hrncic, C.L. Murphy, G. Gallo, D.T. Weiss, and A. Solomon, Microtechniques and Purification Techniques Application to Chemical Characterization of Amyloid Proteins in Minute Amounts of Tissue. V.M.-Y. Lee, J. Wang, and J. Trojanowski, Purification of Paired Helical Filament Tau and Normal Tau from Human Brain Tissue. J.D. Lowenson, S. Clarke, and A.E. Roher, Chemical Modifications of Deposited Amyloid-b Proteins. C. Characterization of Aggregates In Situ and In Vitro: C. Korth, P. Streit, and B. Oesch, Monoclonal Antibodies Specific for Native, Disease-Associated Isoform of Prion Protein. B. Caughey, M. Horiuchi, R. Demaimay, and G. Raymond, Assays of Protease-Resistant Prion Protein and Its Formation. L.M. Sayre, G. Perry, and M.A. Smith, In Situ Methods for Detection and Localization of Markers of Oxidative Stress: Application in Neurodegenerative Disorders. Y. Al-Abed, A. Kapurniotu, and R. Bucala, Advanced Glycation End Products: Detection and Reversal. B.J. Balin, A.G. Loewy, and D.M. Appelt, Analysis of Transglutaminase-Catalyzed Isopeptide Bonds in Paired H


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