DNA sequence specificity is a sub-specialty in the general area of molecular recognition. This area includes macromolecular-molecular interactions (e.g., protein-DNA), oligomer-DNA interacitons (e.g., triple strands), and ligand-DNA interactions (e.g., drug-DNA). It is this latter group of DNA sequence specificity interactions that is the subject of Volumes 1 and 2 of Advances in DNA Sequence Specific Agents. As was the case for Volume 1, Part A also covers methodology, but in Volume 2 we include calorimetric titrations, molecular modeling, X-ray crystallographic and NMR structural studies, and transcriptional assays. Part B also follows the same format as Volume 1 and describes the sequence specificities and covalent and noncovalent interactions of small ligands with DNA. This volume is aimed in general at scientists who have an interest in deciphering the molecular mechanisms for sequence recognition of DNA. The methods have general applicability to small molecules as well as oligomers and proteins, while the examples provide general principles involved in sequence recognition.


For scientists and researchers n the field of DNA sequenencing.

Table of Contents

List of Contributors. Preface (L.H. Hurley, J.B. Chaires). Part I: Methods used to evaluate the molecular basis for sequence specificity. Calorimetric studies of drug-DNA interactions (L.A. Marky et al.). Molecular modeling of drug-DNA interactions: facts and fantasies (J.O. Trent, S. Neidle). X-Ray crystallographic and NMR structural studies of anthracycline snticancer drugs: implication in drug design (A.H.-J. Wang). Transcriptional assay for [robing molecular aspects of drug-DNA interactions (D.R. Phillips). Part II: Sequence Specificity of DNA Interactive Drugs. Molecular recognition of DNA by daunorubicin (J.B. Chaires). Covalent interactions of ethidium and actinomycin D with nucleic acids: photoaffinity labeling of DNA (D.E. Graves). DNA binding of dinuclear platinum complexes (N. Farrell). DNA sequence selectivity of the pyrrole-derived, bifunctional alkylating agents (P.B. Hopkins). Index.


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© 1996
Elsevier Science
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About the editor

J.B. Chaires

Affiliations and Expertise

Department of Biochemistry, University of Mississippi Medical Center, Jackson, MI, USA