Advances in Cell Culture

Contributors to Volume

Preface

Renato Dulbecco

Contents of Previous Volumes

Terminal Differentiation in Cultured Human Epidermal Cells

I. The Process of Terminal Differentiation in Human Epidermis

II. The Process of Terminal Differentiation in Cultured Human Epidermal Cells

III. Extracellular Regulators of Terminal Differentiation

IV. The Structures of the Proteins and the Genes Expressed in Epidermis: A Focus on the Keratins

References

Microvascular Endothelial Cells: Isolation, Identification, and Cultivation

I. Introduction 36

II. Isolation and Cultivation of Microvascular Endothelial Cells from Different Vascular Beds

III. Identification of Microvascular Endothelial Cells

IV. Special Techniques and Culture Ingredients Useful in the Isolation and Culture of Microvessel Endothelial Cells

References

The Production of Artemisinin in Artemisia annua L Tissue Cultures

I. Introduction

II. Artemisia Plants

III. Artemisia Tissue Cultures

IV. Summary

References

Plant Cell Suspensions for Screening and Studying the Mode of Action of Plant Growth Retardants

I. General Remarks on the Application of Cell Cultures in Plant Biotechnology

II. Cell Suspension Cultures in Environmental and Agrochemical Research

III. Practical Use of Plant Growth Retardants in Agriculture

IV. Regulation of the Meristematic Activity in Plants by Growth Retardants

V. Heterotrophically Cultivated Cell Suspensions for Imitating the Response of Whole Plants to Growth Retardants

VI. Designing and Establishing a Micromethod for Large-Scale Screening of Growth Retardants

VII. Elucidating the Mode of Action of Growth Retardants

VIII. Summary

IX. Appendix: Abbreviations and Nomenclature of Growth Retardants

References

Cultures of Mammary Epithelial Cells: Extracellular Matrix and Functional Differentiation

I. Introduction

II. Mouse Mammary Epithelial Cells

III. Human Mammary Epithelial Cells

IV. Possible Mechanism of ECM Action

V. Cell Culture Procedures

References

Cell Culture for Testing Anticancer Compounds

I. Introduction

II. Assays of Cell Survival and/or Structural Integrity

III. Assays of Metabolic Integrity

IV. Assays of Functional Integrity/Reproductive Capability

V. Other Assays with Potential for Application to Anticancer Drug Testing

VI. Summary and Conclusions

References

Asbestos and Transfection

I. Introduction

II. Asbestos

III. Asbestos and Disease

IV. Effects of Asbestos in Laboratory Rodents

V. Effects of Asbestos on Organ Cultures

VI. Asbestos-Induced Hemolysis

VII. Toxicity of Asbestos for Cell Cultures

VIII. Uptake of Asbestos by Cells

IX. Transformation of Cultured Cells by Asbestos

X. Asbestos as a Mutagen

XI. Association of Asbestos with Chromosomes and Nucleic Acids

XII. "Insoluble" Facilitators of Transfection

XIII. Roles for Asbestos in Carcinogenesis

XIV. Summary

Note Added in Proof

References

Cell Injury, Differentiation, and Regeneration in Explant, Organ, and Cell Culture Models

I. Introduction and Hypothesis

II. Culture Methods

III. Organ-Specific Cell Injury in Vitro

References

In vitro Genetic Manipulation of Cereals and Grasses

I. Introduction

II. Tissue, Cell, and Protoplast Culture

III. Vectorless Gene Transfer

IV. Agrobacterium-Mediated Gene Transfer

V. Summary

References

Note Added in Proof

Index