Advances in Cell Culture

Advances in Cell Culture

1st Edition - July 28, 1988

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  • Editors: Karl Maramorosch, Gordon H. Sato
  • eBook ISBN: 9781483215068

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Description

Advances in Cell Culture, Volume 6 is a compilation of research papers in the field of cell culture. The contributions reflect the applications of cell culture to biotechnology, to the study of basic mechanisms of cellular behavior, and to the study of pathogens and diseases. This volume contains chapters that deal with the differentiation of human epidermal cells, cell injury, and regeneration in cell culture models; the description of the testing of anticancer compounds in cultured cells; and the interactions of cells and asbestos. Other contributions cover the production in plant tissue culture of the potent antimalarial drug, artemisinin; plant cell suspensions used for studying the mode of action of plant growth retardants; and the in vitro genetic manipulation of cereals and grasses. Also included is a biographical sketch of Nobel Laureate Renato Dulbecco, whose pioneering work on mammalian cell layers has had an enormous impact on cell culture and virology. Cell biologists and researchers who use in vitro techniques will find the book highly informative and insightful.

Table of Contents


  • Contributors to Volume

    Preface

    Renato Dulbecco

    Contents of Previous Volumes

    Terminal Differentiation in Cultured Human Epidermal Cells

    I. The Process of Terminal Differentiation in Human Epidermis

    II. The Process of Terminal Differentiation in Cultured Human Epidermal Cells

    III. Extracellular Regulators of Terminal Differentiation

    IV. The Structures of the Proteins and the Genes Expressed in Epidermis: A Focus on the Keratins

    References

    Microvascular Endothelial Cells: Isolation, Identification, and Cultivation

    I. Introduction 36

    II. Isolation and Cultivation of Microvascular Endothelial Cells from Different Vascular Beds

    III. Identification of Microvascular Endothelial Cells

    IV. Special Techniques and Culture Ingredients Useful in the Isolation and Culture of Microvessel Endothelial Cells

    References

    The Production of Artemisinin in Artemisia annua L Tissue Cultures

    I. Introduction

    II. Artemisia Plants

    III. Artemisia Tissue Cultures

    IV. Summary

    References

    Plant Cell Suspensions for Screening and Studying the Mode of Action of Plant Growth Retardants

    I. General Remarks on the Application of Cell Cultures in Plant Biotechnology

    II. Cell Suspension Cultures in Environmental and Agrochemical Research

    III. Practical Use of Plant Growth Retardants in Agriculture

    IV. Regulation of the Meristematic Activity in Plants by Growth Retardants

    V. Heterotrophically Cultivated Cell Suspensions for Imitating the Response of Whole Plants to Growth Retardants

    VI. Designing and Establishing a Micromethod for Large-Scale Screening of Growth Retardants

    VII. Elucidating the Mode of Action of Growth Retardants

    VIII. Summary

    IX. Appendix: Abbreviations and Nomenclature of Growth Retardants

    References

    Cultures of Mammary Epithelial Cells: Extracellular Matrix and Functional Differentiation

    I. Introduction

    II. Mouse Mammary Epithelial Cells

    III. Human Mammary Epithelial Cells

    IV. Possible Mechanism of ECM Action

    V. Cell Culture Procedures

    References

    Cell Culture for Testing Anticancer Compounds

    I. Introduction

    II. Assays of Cell Survival and/or Structural Integrity

    III. Assays of Metabolic Integrity

    IV. Assays of Functional Integrity/Reproductive Capability

    V. Other Assays with Potential for Application to Anticancer Drug Testing

    VI. Summary and Conclusions

    References

    Asbestos and Transfection

    I. Introduction

    II. Asbestos

    III. Asbestos and Disease

    IV. Effects of Asbestos in Laboratory Rodents

    V. Effects of Asbestos on Organ Cultures

    VI. Asbestos-Induced Hemolysis

    VII. Toxicity of Asbestos for Cell Cultures

    VIII. Uptake of Asbestos by Cells

    IX. Transformation of Cultured Cells by Asbestos

    X. Asbestos as a Mutagen

    XI. Association of Asbestos with Chromosomes and Nucleic Acids

    XII. "Insoluble" Facilitators of Transfection

    XIII. Roles for Asbestos in Carcinogenesis

    XIV. Summary

    Note Added in Proof

    References

    Cell Injury, Differentiation, and Regeneration in Explant, Organ, and Cell Culture Models

    I. Introduction and Hypothesis

    II. Culture Methods

    III. Organ-Specific Cell Injury in Vitro

    References

    In vitro Genetic Manipulation of Cereals and Grasses

    I. Introduction

    II. Tissue, Cell, and Protoplast Culture

    III. Vectorless Gene Transfer

    IV. Agrobacterium-Mediated Gene Transfer

    V. Summary

    References

    Note Added in Proof

    Index

Product details

  • No. of pages: 360
  • Language: English
  • Copyright: © Academic Press 1988
  • Published: July 28, 1988
  • Imprint: Academic Press
  • eBook ISBN: 9781483215068

About the Editors

Karl Maramorosch

Professor Karl Maramorosch works at Rutgers University, New Brunswick, NJ, USA.

Affiliations and Expertise

Rutgers University, New Brunswick, NJ, USA

Gordon H. Sato

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