Advances in Cell Culture

Advances in Cell Culture

1st Edition - January 20, 1987

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  • Editor: Karl Maramorosch
  • eBook ISBN: 9781483215051

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Advances in Cell Culture, Volume 5 is a compilation of research papers in the field of cell culture. The contributions reflect the thinking and accomplishments of those who are in the forefront of the broad field of cell culture. This volume contains chapters that describe hybrids of pancreatic islet and insulinoma cells; cultured chondrocytes and their applications in pharmacology; human blood cells for studying measles virus replication; and genetic studies of influenza virus in cultured cells. The rapid cultivation of various species of trees from isolated plant cells and the diverse applications of tree tissue culture are the focus of one chapter. The uses of invertebrate cell lines of mosquitoes, Drosophila, and lepidopteran species as tools for research in physiology, development, and genetics as well as for biochemical and hormonal studies are discussed in three chapters. Another contribution covers the in vitro cultivation of avian coccidia. The volume ends with a historical account of the development of cell banking and of quality control. Also included is a biographical sketch of Harry Eagle, whose pioneering work on defined media has had an enormous impact on cell culture. Cell biologists and researchers who use in vitro techniques will find the book highly informative and insightful.

Table of Contents

  • Preface

    Harry Eagle

    Hybrid Cell Lines Established by Fusing Pancreatic Islet Cells with Insulinoma Cells

    I. Introduction

    II. Hybridization between Pancreatic Islet Cells and Insulinoma Cells

    III. Repeated Hybridization between Hybrid Cells (HIP Series) and Either Islet Cells or Insulinoma Cells

    IV. Future Intentions


    Articular Chondrocytes in Culture: Applications in Pharmacology

    I. Introduction

    II. Physiology of Articular Chondrocytes in Vivo

    III. Maintenance of Main Functions of Articular Chondrocytes in Different Types of Culture

    IV. Culture Media

    V. Applications in Pharmacology

    VI. Conclusions


    Human Blood Cells for Measles Virus Replication

    I. Introduction

    II. Isolation of Lymphocytes

    III. Measles Virus Infection in Blood Cells

    IV. Detection of Virus-Specific RNA

    V. Measles Virus Proteins in Lymphocytes

    VI. Effects of Interferon on Measles Virus Replication

    VII. Summary and Conclusions


    Genetic Changes in the Influenza Viruses during Growth in Cultured Cells

    I. Introduction

    II. Laboratory Cultivation of Influenza Viruses prior to the Use of Cultured Cells

    III. Growth and Assay of the Influenza Viruses in Chick Embryo Fibroblasts and in Established Cell Lines

    IV. Morphology and Structure of Influenza Virions

    V. Host Cell Functions Which Affect Influenza Virus Replication

    VI. Mutations in the HA Which Affect Influenza Virus Host Range

    VII. Effects of Mutations and Selection on Virus Populations in Cell Culture

    VIII. Concluding Remarks


    The Biochemistry and Genetics of Mosquito Cells in Culture

    I. Introduction

    II. Aedes albopictus Cell Lines

    III. Biochemical Properties

    IV. Mutant Cell Clones

    V. DNA-Mediated Gene Transfer

    VI. Host-Virus Interactions


    Hormonal Regulation of Fat Body Function in Vitro

    I. Introduction

    II. Vitellogenesis in Locusts

    III. Vitellogenesis in Ovoviviparous Cockroaches

    IV. Vitellogenesis in Drosophila

    V. Hemoglobin and Yolk Protein Synthesis in Chironomus

    VI. Vitellogenesis in Aedes aegypti


    Lepidopteran Cell Lines: Tools for Research in Physiology, Development, and Genetics

    I. Introduction

    II. Importance of Research on Lepidopteran Cell Lines

    III. Current Status of Lepidopteran Cell Lines

    IV. Recent Progress and Prospects


    Tree Tissue Culture Applications

    I. Introduction

    II. Cloning

    III. Somatic Genetic Modification

    IV. Cold Storage of Cultures

    V. Disease Control

    VI. Cell Metabolism and Secondary Products

    VII. Conclusion


    In Vitro Cultivation of the Avian Coccidia

    I. Introduction

    II. Common Constraints on the in Vitro Cultivation of the Avian Coccidia and Alternative Approaches

    III. Discussion


    The Development of Cell Banking in the United States 1960-1985: A Strategic Approach to Quality Control

    I. Virus and Cancer Panel Objectives

    II. Current Policies of the Cell Culture Collection Committee



Product details

  • No. of pages: 314
  • Language: English
  • Copyright: © Academic Press 1987
  • Published: January 20, 1987
  • Imprint: Academic Press
  • eBook ISBN: 9781483215051

About the Editor

Karl Maramorosch

Professor Karl Maramorosch works at Rutgers University, New Brunswick, NJ, USA.

Affiliations and Expertise

Professor, Rutgers University, New Brunswick, NJ, USA

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