Advances in Cell Culture

Contributors to Volume 4

Preface

George Gey

Contents of Previous Volumes

Human Myopathies in Muscle Culture: Morphological, Cytochemical, and Biochemical Studies

I. Introduction

II. Hereditary Diseases of Muscle

III. Acquired Diseases of Muscle

IV. Experimental Viral Myopathies

V. Conclusion

References

The Biology of Human Colon Tumor Cells in Culture

I. Introduction

II. Biological Properties of Human Tumor Cell Lines

III. Biological Properties of Cell Lines LS174T and LS180

IV. Antigenic Analysis of LS174T and LS180 Cells

V. Summary and Conclusions

References

Cell Shape and Growth Control

I. Introduction

II. Methods of Cell Shape Analysis

III. Shape Changes in Models for Epithelial Transformation

IV. Conclusions

References

Hybridoma Technology

I. Introduction

II. Utilization

III. Immunization

IV. Fusion

V. Cloning

VI. Freezing and Reconstitution

VII. Production of Antibody

VIII. Characterization of Immunoglobulin Classes

IX. Monoclonality, Epitope Screening, and Affinity

X. Screening Assays

XI. Storage of Antibodies

XII. Human Monoclonal Antibody Production

XIII. Laboratory Setup

References

Morphogenesis of Mitochondria during Spermiogenesis in Drosophila Organ Culture

I. Introduction

II. Historical Outline

III. Mitochondrial Morphogenesis during Spermiogenesis of Drosophila in Vitro

IV. Regulation of Morphogenesis and Function of the Nebenkern Derivatives

References

Effects of Mycotoxins on Invertebrate Cells in Vitro

I. Introduction

II. Toxic Actions of Fungus Culture Filtrates

III. Action of Substances Extracted with Chloroform from Culture Filtrates of Fungi or Hemolymph of Infected Insects

IV. Destruxin Action

V. Conclusions

References

Microcarriers in Cell Culture: Structure and Applications

I. Introduction

II. Advantages of the Microcarrier Culturing Systems

III. The Development of the Microcarriers

IV. Commercial Microcarriers

V. Noncommercial Microcarriers

VI. Important Parameters in the Microcarrier Composition

VII. Applications of Microcarrier Cultures

VIII. Concluding Remarks

References

The Establishment of Cell Lines from Human Solid Tumors

I. Introduction

II. Establishment of Cell Lines

III. Chemotherapeutic Screens

IV. Summary and Future Perspectives

References

Index