Advances in Cell Culture

Advances in Cell Culture

1st Edition - May 1, 1984

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  • Editor: Karl Maramorosch
  • eBook ISBN: 9781483215037

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Description

Advances in Cell Culture, Volume 3 is a compilation of articles in the field of cell culture. The chapters in the book are prepared by recognized authorities in their specialized fields. The text deals with all the aspects of cell culture, which includes the growth of individual cells or cell populations, the growth of small fragments of explanted tissue, the growth of organs, and the growth of obligate parasites in cell culture systems. This volume provides reviews of important aspects of in vitro cultivation and presents the increasing understanding of the wide ramifications of in vitro techniques. The book also focuses on specific topics in cell culture such as protoplasts and plant viruses; applications of flow cytometry to tissue culture systems; plant tissue culture systems for screening of plant growth regulators; growth factors and hemopoietic cell differentiation; and mosquito cell culture. A chapter is also devoted to a biographical sketch of Philip R. White, remembered for his pioneering plant tissue culture work and his contributions to animal cell culture and to in vitro research as a whole. Cell biologists and researchers who use in vitro techniques will find the book highly informative and insightful.

Table of Contents


  • Contributors to Volume 3

    Preface

    Philip Rodney White

    Contents of Previous Volumes

    Protoplasts and Plant Viruses

    I. Virus Infection of Plant Protoplasts

    II. Virus Replication in Plant Protoplasts

    III. Resistance to Virus Infection

    IV. In Vitro Methods for Recovering Virus Resistance

    V. Perspectives and Problems for Genetic Engineering

    References

    Applications of Flow Cytometry to Tissue Culture Systems

    I. Introduction

    II. What Is Flow Cytometry?

    III. Cell Kinetic Analysis

    IV. Chromosome Analysis

    V. Cell Marker Analysis

    VI. Cell Functional Analysis

    VII. Detection and Cloning of Mutant and Hybrid Cells

    VIII. Conclusions

    References

    Plant Tissue Culture Systems for Screening of Plant Growth Regulators: Hormones, Herbicides, and Natural Phytotoxins

    I. Introduction: General Considerations

    II. Screening for Hormonal Activities

    III. Screening for Yield Enhancement

    IV. Screening Phytotoxic Compounds

    V. Drift, Instability, Habituation, Phenotypic Adaptations, and Other Problems

    VI. Concluding Remarks

    VII. Appendix: Nomenclature of Growth Regulators

    References

    Microsporida in Cell Culture

    I. Introduction

    II. A Description of the Microsporida

    III. Establishing Microsporida in Cell Cultures

    IV. Observations of Microsporida in Cell Cultures

    V. Other Techniques

    VI. Uses of Microsporida in Cell Cultures

    VII. Conclusion

    References

    Growth Factors and Hemopoietic Cell Differentiation

    I. Blood Cell Diversity

    II. Colony Stimulating Factors: Nomenclature

    III. Stem Cell Heterogeneity

    IV. Production of Myeloid Cells M-CFC, G-CFC, and GM-CFC

    V. Erythroid Cells

    VI. Megakaryocyte and Eosinophil Production

    VII. Production of Lymphocytes

    VIII. Leukemic Cells

    IX. Growth Factors

    X. Growth Factors for Multipotential Stem Cells

    XI. GM-CSF, M-CSF, and G-CSF

    XII. Erythropoietic Stimuli

    XIII. MEG-CSF and EO-CSF

    References

    Mosquito Cell Culture

    I. Introduction

    II. Cell Lines

    III. Growth Requirements

    IV. Characteristics

    V. Clonal Analyses

    VI. Conclusions

    References

    The Coming of Age of WI-38

    I. The Birth of WI-38

    II. Distribution of WI-38

    III. Who Owns WI-38?

    IV. The Mortality of Cultured Normal Cells

    V. The Great Virus Vaccine Substrate Debate

    VI. Advances in Cytogerontology

    References

    Index

Product details

  • No. of pages: 242
  • Language: English
  • Copyright: © Academic Press 1984
  • Published: May 1, 1984
  • Imprint: Academic Press
  • eBook ISBN: 9781483215037

About the Editor

Karl Maramorosch

Professor Karl Maramorosch works at Rutgers University, New Brunswick, NJ, USA.

Affiliations and Expertise

Rutgers University, New Brunswick, NJ, USA

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