Advances in Cell Culture - 1st Edition - ISBN: 9780120079025, 9781483268194

Advances in Cell Culture, Volume 2

1st Edition

Editors: Karl Maramorosch
eBook ISBN: 9781483268194
Imprint: Academic Press
Published Date: 28th October 1982
Page Count: 350
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Table of Contents

Contributors to Volume 2


Richard Benedict Goldschmidt

Cellular Senescence in Vitro

I. Introduction: Definitions of Aging

II. Historical Background

III. Characteristics of Cell Growth in Culture

IV. Cellular Morphology in Aging Cultures

V. Macromolecular Synthesis

VI. Proposed Mechanisms of Cellular Senescence

VII. Factors Modulating Replicative Life Span

VIII. Conclusions


Introduction and Expression of Foreign DNA Sequences in Mammalian Cells

I. Introduction

II. Methods of Introducing Genes

III. Vectors for Introduction of DNA

IV. Factors That Affect Gene Transfer

V. Fate of DNA in the Recipient Cell

VI. Transfer of Selectable and Nonselectable Genes

VII. Expression of Foreign Genes in Mammalian Cells

VIII. Rescue and Recovery of DNA Sequences

IX. Future of DNA Transfer Systems


Detection of Mycoplasmal Infection of Cell Cultures

I. Introduction

II. Methods to Detect Cell Culture Mycoplasmas

III. Mycoplasmal Infection of Differentiated Cell Cultures

IV. Identification of Isolates

V. Frequency of Mycoplasmal Assays

VI. Conclusion


Media for Insect Cell Cultures

I. Introduction

II. Nutritional Requirements and Constituents of Media

III. Media with Hemolymph or Sera

IV. Media Free of Hemolymph or Sera

V. Application of Vertebrate Culture Media to Insect Cell Cultures

VI. Epilogue


Insect Ovaries and Follicles in Culture: Oocyte and Early Embryonic Development in Pedogenetic Gall Midges

I. Introduction

II. Test Systems and Culture Techniques

III. Regular Oogenesis and Early Embryonic Development

IV. Irregular Oogenesis and Early Embryonic Development

V. Summary and Conclusions


Morphogenesis in Insect Tissue Culture

I. Introduction

II. Cell Culture

III. Organ Culture

IV. Conclusions


Agricultural Applications of in Vitro Plant Cultivation

I. Introduction

II. Current Status

III. Future Applications

IV. Changing Philosophy in Agricultural Research


Fish Cell Culture

I. Introduction

II. Physiological Salines

III. Culture Media

IV. Methods

V. Applications of Fish Cell Cultures




Advances in Cell Culture, Volume 2 is a compendium of critical reviews in the field of cell culture. The chapters in the book are prepared by recognized authorities in their specialized fields. The text deals with all the aspects of cell culture, which includes the growth of individual cells or cell populations, the growth of small fragments of explanted tissue, the growth of organs, and the growth of obligate parasites in cell culture systems.

This volume provides reviews of important aspects of in vitro cultivation and presents the increasing understanding of the wide ramifications of in vitro techniques. The book also focuses on specific topics in cell culture such as cellular senescence in vitro; introduction and expression of foreign DNA sequences in mammalian cells; media for insect cell cultures; agricultural applications of in vitro plant cultivation; and fish cell culture. A short biographical note devoted to Ross G. Harrison, whose insight and pioneering work led to the foundation of animal cell culture is likewise included.

Cell biologists and researchers who use in vitro techniques will find the book highly informative and insightful.


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© Academic Press 1982
Academic Press
eBook ISBN:

Ratings and Reviews

About the Editors

Karl Maramorosch Editor

Professor Karl Maramorosch works at Rutgers University, New Brunswick, NJ, USA.

Affiliations and Expertise

Rutgers University, New Brunswick, NJ, USA