Advances in Cell Culture

Advances in Cell Culture

1st Edition - May 28, 1981

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  • Editor: Karl Maramorosch
  • eBook ISBN: 9781483215020

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Advances in Cell Culture, Volume 1 is a compendium of critical reviews in the field of cell culture. The chapters in the book are prepared by recognized authorities in their specialized fields. The text deals with all the aspects of cell culture, which includes the growth of individual cells or cell populations, the growth of small fragments of explanted tissue, the growth of organs, and the growth of obligate parasites in cell culture systems. This volume contains research papers that discuss vertebrate, invertebrate, and plant cell culture, as well as the cultivation of obligate parasites. The book also focuses on specific topics in cell culture such as the past and future developments in the field of genetic manipulation; evaluation of antiviral compounds in cell cultures with acute or persistent virus infections; propagation of malaria parasites in vitro; regulation of plant organogenesis; and insect cells for insect virus production. Cell biologists and researchers who use in vitro techniques will find the book highly informative and insightful.

Table of Contents

  • Contributors to Volume 1


    Ross Granville Harrison

    Hybrids and Viruses: Reflections on Golden Past and Less Certain Future



    Regulation of Glycopeptide Hormone Synthesis in Cell Culture

    I. Eutopic and Ectopic Production of Proteins in Cell Culture as Models for Studies on Gene Regulation

    II. Induction of Glycopeptide Hormone Synthesis in HeLa 65 Cell Cultures by Aliphatic Monocarboxylates

    III. Differences in the Regulation of Eutopic and Ectopic Synthesis of Glycopeptide Hormone in Culture

    IV. Histone Modification Mediated by Sodium Butyrate

    V. Induction of Glycopeptide Hormone Synthesis by Antineoplastic Drugs

    VI. Cell Cycle Analysis of Sodium Butyrate and Hydroxyurea Effects on HeLa Cells

    VII. Summary and Conclusions


    Evaluation of Antiviral Compounds in Cell Cultures with Acute or Persistent Virus Infections

    I. Introduction

    II. Characteristics of Antiviral Compounds Which Can Be Assessed in Vitro

    III. Tests in Cultures with Acute Virus Infections

    IV. Tests in Persistently Infected Cell Lines

    V. Standardization of Test Procedures

    VI. Summary and Conclusions


    The Effects of Ecdysteroid Hormones on Drosophila Melanogaster Cell Lines

    I. Introduction

    II. Origin and General Characteristics of the Cell Lines

    III. The Ecdysteroid Response

    IV. The Effect of Juvenile Hormone on the Ecdysteroid Response

    V. Ecdysteroid-Responsive Genes

    VI. Ecdysteroid Receptors

    VII. Conclusions


    Drosophila Cells and Cell Lines

    I. Introduction

    II. Primary Cultures

    III. Cell Lines

    IV. Cell Biochemistry

    V. Experiments Using Cells

    VI. Summary and Conclusions


    Propagation of Malaria Parasites in Vitro

    I. Introduction

    II. Culture of Invertebrate Inhabiting Stages of Malaria Parasites

    III. Culture of Vertebrate Inhabiting Stages of Malaria Parasites

    IV. Summary and Conclusions


    Regulation of Plant Organogenesis

    I. Introduction

    II. Regulation by Manipulation

    III. Regulation at the Tissue Level

    IV. Regulation at the Biochemical Level

    V. Regulation at the Cellular and Molecular Levels

    VI. Summary

    VII. Concluding Thoughts


    Protoplasts as Vehicles for Plant Propagation and Improvement

    I. Introduction

    II. Isolation and Cultivation of Plant Protoplasts

    III. Protoplasts in Genetic Manipulation


    Insect Cells for Insect Virus Production

    I. Introduction

    II. Availability of Cell Lines or Strains

    III. Suspension Culture Systems for Virus Production

    IV. Substrate Systems for Virus Production

    V. Factors Affecting the Virus Yield in Cell Cultures

    VI. Quality of Cell Culture Produced Viruses

    VII. Conclusions


    Gastropod Cells in Vitro

    I. Background

    II. Establishment and Maintenance of Cell Cultures

    III. Uses of Cell Cultures

    IV. Prospect

    V. Summary



Product details

  • No. of pages: 358
  • Language: English
  • Copyright: © Academic Press 1981
  • Published: May 28, 1981
  • Imprint: Academic Press
  • eBook ISBN: 9781483215020

About the Editor

Karl Maramorosch

Professor Karl Maramorosch works at Rutgers University, New Brunswick, NJ, USA.

Affiliations and Expertise

Rutgers University, New Brunswick, NJ, USA

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