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Adhesion of Microbial Pathogens - 1st Edition - ISBN: 9780121821548, 9780080883663

Adhesion of Microbial Pathogens, Volume 253

1st Edition

Editors in Chief: John Abelson Melvin Simon
Serial Volume Editors: Ron Doyle Itzhak Ofek
eBook ISBN: 9780080883663
Imprint: Academic Press
Published Date: 11th July 1995
Page Count: 600


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Table of Contents

General Methods for Adhesion to Animal Cells: E. Rozdzinski and E. Tuomanen, Adhesion of Microbial Pathogens to Leukocyte Integrins: Methods to Study Ligand Mimicry. R.F. Rest, Association of Bacteria with Human Phagocytes. R. Malaviya and S.N. Abraham, Interaction of Bacteria with Mast Cells. J. Goldhar, Erythrocytes as Target Cells for Testing Bacterial Adhesins. K.A. Krogfelt, Adhesin-Dependent Isolation and Characterization of Bacteria from Their Natural Environment. L.D. Hazlett, Analysis of Ocular Microbial Adhesion. Identification of Adhesins and Receptors: F.J. Cassels and H. van Halbeek, Isolation and Structural Characterization of Adhesin Polysaccharide Receptors. N. Sharon and I. Ofek, Identification of Receptors for Bacterial Lectins by Blotting Techniques. W.A. Petri, Jr., and R.L. Schnaar, Purification and Characterization of Galactose- and N-Acetylgalactosamine-Specific Adhesin Lectin of Entamoeba histolytica. G. Magnusson, S.J. Hultgren, and J. Kihlberg, Specificity Mapping of Bacterial Lectins by Inhibition of Hemagglutination Using Deoxy and Deoxyfluoro Analogs of Receptor-Active Saccharides. K.K. Lee, W.Y. Wong, H.B. Sheth, R.S.Hodges, W. Paranchych, and R.T. Irvin, Use of Synthetic Peptides in Characterization of Microbial Adhesins. A. Prakobphol, H. Leffler, and S.J. Fisher, Identifying Bacterial Receptor Proteins and Quantifying Strength of Interactions They Mediate. General Methods for Visualization and Data Presentation of Adhesion: S. Knutton, Electron Microscopical Methods in Adhesion. P.A. Manning, Use of Confocal Microscopy in Studying Bacterial Adhesion and Invasion. J.R. Johnson, Epidemiological Considerations in Studies of Microbial Adhesion. M.M. Cowan, Kinetic Analysis of Microbial Adhesion. A.G. Matthysse, Observation and Measurement of Bacterial Adhesion to Plants. H. Leffler, W. Agace, S. Hedges, R. Lindstedt, M. Svensson, and C. Svanborg, Strategies for Studying Bacterial Adhesion in Vivo E.V. Sokurenko and D.L. Hasty, Assay for Adhesion of Host Cells to Immobilized Bacteria. Molecular Biology of Adhesins: V. Vetter and J. Hacker, Strategies for Employing Molecular Genetics to Study Tip Adhesins. D.M. Schifferli, Use of Tn phoA and T7 RNA Polymerase to Study Fimbrial Proteins. S.I. Hull and R.A. Hull, Molecular Cloning of Adhesion Genes. E. Hanski, G. Fogg, A. Tovi, N. Okada, I. Burstein, and M. Caparon, Molecular Analysis of Streptococcus pyogenes Adhesion. Methods for Study of Adhesion of Selected Bacteria: P.S. Cohen and D.C. Laux, Bacterial Adhesion to and Penetration of Intestinal Mucus in Vitro. E.A. Dean-Nystrom, Identification of Intestinal Receptors for Enterotoxigenic Escherichia coli. M.S. Donnenberg and J.P. Nataro, Methods for Studying Adhesion of Diarrheagenic Escherichia coli. D.G. Evans and D.J. Evans, Jr., Adhesion Properties of Helicobacter pylori. H.L.T. Mobley, G.R. Chippendale, and J.W. Warren, In Vitro Adhesion of Bacteria to Exfoliated Uroepithelial Cells: Criteria for Quantitative Analysis. I. Kahane, Adhesion of Mycoplasmas. D.H. Meyer and P.M.Fives-Taylor, Adhesion of Oral Bacteria to Soft Tissue. P.E. Kolenbrander, Coaggregations among Oral Bacteria. J. London, Identifying and Isolating Fimbrial-Associated Adhesins of Oral Gram-Negative Bacteria. Adhesion of Eukaryotic Pathogens: J.F. Alderete, R. Arroyo, and M.W. Lehker, Analysis for Adhesins and Specific Cytoadhesion of Trichomonas vaginalis. K.C. Hazen and P.M. Glee, Adhesion of Fungi. K.C. Kain and J.I. Ravdin, Galactose-Specific Adhesion Mechanisms of Entamoeba histolytica: Model for Study of Enteric Pathogens. E. Segal and H. Sandovsky-Losica, Adhesion and Interaction of Candida albicans with Mammalian Tissues in Vitro and in Vivo. Methods for Study of Adhesion to Solid Surfaces: H.J. Busscher and H.C. van der Mei, Use of Flow Chamber Devices and Image Analysis Methods to Study Microbial Adhesion. G.D. Christensen, L. Baldassarri, and W.A. Simpson, Methods for Studying Microbial Colonization of Plastics. A. Ljungh and T. Wadstrim, Binding of Extracellular Matrix Proteins by Microbes. G. Reid, Adhesion of Urogenital Organisms to Polymers and Prosthetic Devices. E.V. Sokurenko, V.A. McMackin, and D.L. Hasty, Bacterial Adhesion Measured by Growth of Adherent Organisms. I. Ofek, Enzyme-Linked Immunosorbent-Based Adhesion Assays. K.M. Schilling and R.J. Doyle, Bacterial Adhesion to Hydroxylapatite. R.J. Doyle and M. Rosenberg, Measurement of Microbial Adhesion to Hydrophobic Substrata. Author Index. Subject Index.


The critically acclaimed laboratory standard for forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerlyawaited, frequently consulted, and praised by researchers and reviewers alike. More than 250 volumes have been published (all of them still in print) and much of the material is relevant even today--truly an essential publication for researchers in all fields of life sciences.

Key Features

@introbul:Key Features @bul:* Strategies involved in studying adhesion

  • Genetic manipulation of adhesions
  • Adhesion and receptor isolation and characterization
  • Distinguishes between adhesion and invasion
  • Assays for adhesion
  • Kinetic and epidemiological considerations of adhesion and infection


Microbiologists, biochemists, molecular biologists, pathologists, cell biologists, and epidemiologists.


No. of pages:
© Academic Press 1995
11th July 1995
Academic Press
eBook ISBN:


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About the Editors in Chief

John Abelson

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA

About the Serial Volume Editors

Ron Doyle

Affiliations and Expertise

Schools of Dentistry and Medicine, University of Louisville, Kentucky, U.S.A.

Itzhak Ofek

Affiliations and Expertise

Sackler School of Medicine, Tel Aviv University, Ramat-Aviv, Israel