This volume emphasizes methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins (G proteins), and guanylyl cyclases.
@introbul:Key Features @bul:* Adenylyl cyclase: determination of activity
- Preparation of materials useful in purification of components of hormonally responsive cyclase systems
- Purification guanine nucleotide-dependent regulatory proteins
- Purification and characterization of g proteins: labeling and quantitating of G proteins
- Reconstitution guanylyl cyclase: assay
- Preparation of materials used for study of the cyclase
Biochemists, endocrinologists, cell and molecular biologists, neuroscientists, analytical and clinical chemists, biomedical researchers, pharmacologists, and physiologists.
Adenylyl Cyclase: Determination of Adenylyl Cyclase Activity:
R.A. Johnson and Y. Salomon, Assay of Adenylyl Cyclase Catalytic Activity.
Y. Salomon, Cellular Responsiveness to Hormones and Neurotransmitters: Conversion of [3H]Adenine to [3H]cAMP in Cell Monolayers, Cell Suspensions, and Tissue Slices.
Preparation of Materials Useful in Purification of Components of Hormonally Responsive Adenyl Cyclase Systems:
T.F. Walseth, P.S.T. Yuen, and M.C. Moos, Jr. , Preparation of ~ga-32P-Labeled Nucleoside Triphosphates, Nicotinamide Adenine Dinucleotide, and Cyclic Nucleotides for Use in Determining Adenylyl and Guanylyl Cyclases and Cyclic Nucleotide Phosphodiesterases.
T. Pfeuffer, Synthesis of Forskolin-Agarose Affinity Matrices.
A. Laurenza and K.B. Seamon, High-Affinity Binding Sites for [3H]Forskolin.
Purification of Adenylyl Cyclases:
P. Wang and D.R. Storm, Purification and Characterization of Calmodulin-Sensitive Adenylyl Cyclase from Bovine Brain.
E. Pfeuffer, S. Mollner, and T. Pfeuffer, Purification of Adenylyl Cyclase from Heart and Brain.
W.A. Toscano, Jr., and M.K. Gross, Calmodulin-Mediated Adenylyl Cyclase from Equine Sperm.
G.B. Rosenberg, P. Wang, and D.R. Storm, Isolation of Polyclonal Antibodies against Bovine Brain Calmodulin-Sensitive Adenylyl Cyclases.
S. Mollner and T. Pfeuffer, Characteristics and Use of Monoclonal Antibodies to Various Forms of Adenylyl Cyclase.
T. Braun, Purification of Soluble Form of Adenylyl Cyclase from Testes.
H.R. Masure, M.G. Donovan, and D.R. Storm, Purification and Assay of Cell-Invasive Form of Calmodulin-Sensitive Adenylyl Cyclase from Bordetella pertussis.
- No. of pages:
- © Academic Press 1991
- 4th February 1991
- Academic Press
- eBook ISBN:
- Hardcover ISBN:
School of Medicine Health Sciences Center, University of New York, Stony Brook, U.S.A.
Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, Tennessee, U.S.A.
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