Description

This volume emphasizes methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins (G proteins), and guanylyl cyclases.

Key Features

@introbul:Key Features @bul:* Adenylyl cyclase: determination of activity * Preparation of materials useful in purification of components of hormonally responsive cyclase systems * Purification guanine nucleotide-dependent regulatory proteins * Purification and characterization of g proteins: labeling and quantitating of G proteins * Reconstitution guanylyl cyclase: assay * Preparation of materials used for study of the cyclase * Purification * Regulation

Readership

Biochemists, endocrinologists, cell and molecular biologists, neuroscientists, analytical and clinical chemists, biomedical researchers, pharmacologists, and physiologists.

Table of Contents

Adenylyl Cyclase: Determination of Adenylyl Cyclase Activity: R.A. Johnson and Y. Salomon, Assay of Adenylyl Cyclase Catalytic Activity. Y. Salomon, Cellular Responsiveness to Hormones and Neurotransmitters: Conversion of [3H]Adenine to [3H]cAMP in Cell Monolayers, Cell Suspensions, and Tissue Slices. Preparation of Materials Useful in Purification of Components of Hormonally Responsive Adenyl Cyclase Systems: T.F. Walseth, P.S.T. Yuen, and M.C. Moos, Jr. , Preparation of ~ga-32P-Labeled Nucleoside Triphosphates, Nicotinamide Adenine Dinucleotide, and Cyclic Nucleotides for Use in Determining Adenylyl and Guanylyl Cyclases and Cyclic Nucleotide Phosphodiesterases. T. Pfeuffer, Synthesis of Forskolin-Agarose Affinity Matrices. A. Laurenza and K.B. Seamon, High-Affinity Binding Sites for [3H]Forskolin. Purification of Adenylyl Cyclases: P. Wang and D.R. Storm, Purification and Characterization of Calmodulin-Sensitive Adenylyl Cyclase from Bovine Brain. E. Pfeuffer, S. Mollner, and T. Pfeuffer, Purification of Adenylyl Cyclase from Heart and Brain. W.A. Toscano, Jr., and M.K. Gross, Calmodulin-Mediated Adenylyl Cyclase from Equine Sperm. G.B. Rosenberg, P. Wang, and D.R. Storm, Isolation of Polyclonal Antibodies against Bovine Brain Calmodulin-Sensitive Adenylyl Cyclases. S. Mollner and T. Pfeuffer, Characteristics and Use of Monoclonal Antibodies to Various Forms of Adenylyl Cyclase. T. Braun, Purification of Soluble Form of Adenylyl Cyclase from Testes. H.R. Masure, M.G. Donovan, and D.R. Storm, Purification and Assay of Cell-Invasive Form of Calmodulin-Sensitive Adenylyl Cyclase from Bordetella pertussis. S.H. Lepp

Details

No. of pages:
511
Language:
English
Copyright:
© 1991
Published:
Imprint:
Academic Press
eBook ISBN:
9780080883106
Print ISBN:
9780121820961

About the serial-volume-editors

Roger Johnson

Affiliations and Expertise

School of Medicine Health Sciences Center, University of New York, Stony Brook, U.S.A.

Jackie Corbin

Affiliations and Expertise

Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, Tennessee, U.S.A.

Reviews

@from:Praise for the Volume @qu:"Highly recommended. Methods books are an essential aid to the laboratory worker and this series has a well-deserved international reputation and requires no introduction. As usual, the book is very well presented and the list of contributing authors is extremely impressive. @source:--NEUROSCIENCE @from:Praise for the Series @qu:"The Methods in Enzymology series represents the gold-standard." @source:--NEUROSCIENCE @qu:"Incomparably useful." @source:--ANALYTICAL BIOCHEMISTRY @qu:"It is a true 'methods' series, including almost every detail from basic theory to sources of equipment and reagents, with timely documentation provided on each page." @source:--BIO/TECHNOLOGY @qu:"The series has been following the growing, changing and creation of new areas of science. It should be on the shelves of all libraries in the world as a whole collection." @source:--CHEMISTRY IN INDUSTRY @qu:"The appearance of another volume in that excellent series, Methods in Enzymology, is always a cause for appreciation for those who wish to successfully carry out a particular technique or prepare an enzyme or metabolic intermediate without the tiresome prospect of searching through unfamiliar literature and perhaps selecting an unproven method which is not easily reproduced." @source:--AMERICAN SOCIETY OF MICROBIOLOGY NEWS @qu:"If we had some way to find the work most often consulted in the laboratory, it could well be the multi-volume series Methods in Enzymology...a great work." @source:--ENZYMOLOGIA @qu:"A series that has established itself as a definitive reference for biochemists." @source:--JOURNAL OF CHROMATOGRAPHY