Adenylyl Cyclase, G Proteins, and Guanylyl Cyclase, Volume 195

1st Edition

Serial Volume Editors: Roger Johnson Jackie Corbin
Editor-in-Chiefs: John Abelson Melvin Simon
Hardcover ISBN: 9780121820961
eBook ISBN: 9780080883106
Imprint: Academic Press
Published Date: 4th February 1991
Page Count: 511
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Institutional Access

Table of Contents

Adenylyl Cyclase: Determination of Adenylyl Cyclase Activity:

R.A. Johnson and Y. Salomon, Assay of Adenylyl Cyclase Catalytic Activity.

Y. Salomon, Cellular Responsiveness to Hormones and Neurotransmitters: Conversion of [3H]Adenine to [3H]cAMP in Cell Monolayers, Cell Suspensions, and Tissue Slices.

Preparation of Materials Useful in Purification of Components of Hormonally Responsive Adenyl Cyclase Systems:

T.F. Walseth, P.S.T. Yuen, and M.C. Moos, Jr. , Preparation of ~ga-32P-Labeled Nucleoside Triphosphates, Nicotinamide Adenine Dinucleotide, and Cyclic Nucleotides for Use in Determining Adenylyl and Guanylyl Cyclases and Cyclic Nucleotide Phosphodiesterases.

T. Pfeuffer, Synthesis of Forskolin-Agarose Affinity Matrices.

A. Laurenza and K.B. Seamon, High-Affinity Binding Sites for [3H]Forskolin.

Purification of Adenylyl Cyclases:

P. Wang and D.R. Storm, Purification and Characterization of Calmodulin-Sensitive Adenylyl Cyclase from Bovine Brain.

E. Pfeuffer, S. Mollner, and T. Pfeuffer, Purification of Adenylyl Cyclase from Heart and Brain.

W.A. Toscano, Jr., and M.K. Gross, Calmodulin-Mediated Adenylyl Cyclase from Equine Sperm.

G.B. Rosenberg, P. Wang, and D.R. Storm, Isolation of Polyclonal Antibodies against Bovine Brain Calmodulin-Sensitive Adenylyl Cyclases.

S. Mollner and T. Pfeuffer, Characteristics and Use of Monoclonal Antibodies to Various Forms of Adenylyl Cyclase.

T. Braun, Purification of Soluble Form of Adenylyl Cyclase from Testes.

H.R. Masure, M.G. Donovan, and D.R. Storm, Purification and Assay of Cell-Invasive Form of Calmodulin-Sensitive Adenylyl Cyclase from Bordetella pertussis.

S.H. Lepp


This volume emphasizes methods for the assay, purification, and characterization of adenylyl cyclases, guanine nucleotide-dependent regulatory proteins (G proteins), and guanylyl cyclases.

Key Features

@introbul:Key Features @bul:* Adenylyl cyclase: determination of activity

  • Preparation of materials useful in purification of components of hormonally responsive cyclase systems
  • Purification guanine nucleotide-dependent regulatory proteins
  • Purification and characterization of g proteins: labeling and quantitating of G proteins
  • Reconstitution guanylyl cyclase: assay
  • Preparation of materials used for study of the cyclase
  • Purification
  • Regulation


Biochemists, endocrinologists, cell and molecular biologists, neuroscientists, analytical and clinical chemists, biomedical researchers, pharmacologists, and physiologists.


No. of pages:
© Academic Press 1991
Academic Press
eBook ISBN:
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About the Serial Volume Editors

Roger Johnson Serial Volume Editor

Affiliations and Expertise

School of Medicine Health Sciences Center, University of New York, Stony Brook, U.S.A.

Jackie Corbin Serial Volume Editor

Affiliations and Expertise

Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, Tennessee, U.S.A.

About the Editor-in-Chiefs

John Abelson Editor-in-Chief

Affiliations and Expertise

California Institute of Technology, Division of Biology, Pasadena, U.S.A.

Melvin Simon Editor-in-Chief

Affiliations and Expertise

The Salk Institute, La Jolla, CA, USA