Acidic Proteins of the Nucleus

Acidic Proteins of the Nucleus

1st Edition - January 1, 1974

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  • Editor: Ivan Cameron
  • eBook ISBN: 9780323154123

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Description

Acidic Proteins of the Nucleus focuses on the functional role of acidic nuclear proteins in differential gene expression. Historically, these proteins are referred to as acidic in nature because they are insoluble in dilute mineral acids and their amino acid composition shows a preponderance of acidic over basic amino acid residues. After an introduction to DNA-binding proteins and transcriptional control in prokaryotic and eukaryotic systems, the subsequent chapters describe various approaches for isolating, separating, and characterizing acidic nuclear proteins. The core chapters specifically cover the isolation, fractionation, and characterization of acidic nuclear phosphoproteins, and the role of these proteins in cell proliferation, cell differentiation, and cell cycle. The last two chapters address the role of acidic nuclear protein in binding steroid hormones and in gene regulation. Each chapter contains some previously unpublished work and provides recommendations for future research. This book will be a good reference background for researchers of acidic nuclear proteins.

Table of Contents


  • List of Contributors

    Preface

    1 DNA-Binding Proteins and Transcriptional Control in Prokaryotic and Eukaryotic Systems

    I. Introduction

    II. Transcriptional Control in Prokaryotes—A Model for Higher Organisms

    III. Transcriptional Control by Modification of RNA Polymerases

    IV. Nuclear Nonhistone Proteins and Transcriptional Control in Eukaryotes

    References

    2 Isolation of the Nuclear Acidic Proteins, Their Fractionation, and Some General Characteristics

    I. Introduction

    II. Isolation of Cell Nuclei and Subnuclear Components

    III. Acidic Proteins of the Chromatin

    IV. Acidic Proteins of the Nucleolus

    V. Acidic Proteins of the Nucleoplasm

    VI. Concluding Remarks

    References

    3 Extraction and Characterization of the Phenol-Soluble Acidic Nuclear Proteins

    I. Introduction

    II. Development of Procedure Using Aqueous Phenol to Dissociate and Solubilize the Nuclear Acidic Proteins

    III. Chemical Characteristics and Biochemical Activity of the Phenol-Soluble Residual Acidic Proteins

    IV. Fractionation of the Nuclear Proteins

    V. Preparation of Protein for Electrophoretic Separation

    VI. Electrophoretic Separation of the Phenol-Soluble Proteins

    VII. Concluding Remarks

    References

    4 Acidic Nuclear Phosphoproteins

    I. Introduction b

    II. Isolation and Fractionation of Acidic Nuclear Phosphoproteins

    III. Phosphate Metabolism of Acidic Nuclear Phosphoproteins

    IV. Functional Properties of Acidic Nuclear Phosphoproteins

    V. Role of Acidic Phosphoproteins in Nuclear Function

    VI. Concluding Remarks

    References

    5 Characterization of Nuclear Phosphoproteins in Physarum Polycephalum

    I. Introduction

    II. Phosphate Content of Phosphoproteins

    III. Pulse Labeling in P1

    IV. Labeling of Nuclei in Vitro with [ y- 32P]ATP

    V. Kinetics of Phosphate Turnover

    VI. Phosphorylation during Starvation

    VII. Summary

    References

    6 The Nuclear Acidic Proteins in Cell Proliferation and Differentiation

    I. Introduction

    II. The Heterogeneity of the Nuclear Acidic Proteins and Cell Proliferation

    III. The Major Acidic Nuclear Proteins and Specific Gene Regulation

    IV. The Contractile Proteins of Isolated Chromatin and Considerations of Their Possible Role in the Regulation of Cell

    Proliferation

    References

    7 Nonhistone Proteins of Dipteran Polytene Nuclei

    I. Introduction

    II. Cytology and Cytochemistry of Genome Activity

    III. Chemical Modification of Polytene Chromosome Proteins

    IV. Qualitative and Quantitative Changes in Protein during Gene Activation

    V. The Possible Role of Proteins in Gene Activation

    References

    8 Acidic Nuclear Proteins and The Cell Cycle

    I. Introduction

    II. Synchronous Cell Cycle Systems, a Comparative Analysis

    III. Acidic Proteins of the Nucleus in Relation to the Cell Cycle

    IV. Summary and Conclusions

    References

    9 The Role of Nuclear Acidic Proteins in Binding Steroid Hormones

    I. Introduction

    II. Primary Site of Action of Steroid Hormones

    III. Cytoplasmic Acidic Proteins as Receptors for Steroid Hormones

    IV. Intracellular Distribution of Steroid Hormones

    V. Intranuclear Localization of Steroid Hormones: Chromatin Binding

    VI. Chromatin-Binding Sites for Hormone-Receptor Complexes: "The Acceptor Molecules"

    VII. Identification and Characterization of the "Acceptor" Molecule in Chick Oviduct Chromatin Which Binds the Progesterone-Receptor Complex

    References

    10 The Role of Acidic Proteins in Gene Regulation

    I. Introduction

    II. Isolation and Characterization of Acidic Proteins

    III. Distribution and Specificity of the Acidic Proteins

    IV. Some Metabolic Aspects of Acidic Proteins

    V. The Biological Assessment of the Acidic Proteins

    VI. Gene Regulation in Eukaryotes

    References

    Author Index

    Subject Index






Product details

  • No. of pages: 362
  • Language: English
  • Copyright: © Academic Press 1974
  • Published: January 1, 1974
  • Imprint: Academic Press
  • eBook ISBN: 9780323154123

About the Editor

Ivan Cameron

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