A Practical Guide to the Study of Calcium in Living Cells

A Practical Guide to the Study of Calcium in Living Cells

1st Edition - March 10, 1994

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  • Editor: Richard Nuccitelli
  • Paperback ISBN: 9780125228107
  • eBook ISBN: 9780080859385
  • Hardcover ISBN: 9780125641418

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A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.

Key Features

  • Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes
  • Fluorescent dyes
  • Luminescent proteins
  • Includes techniques for perturbing intracellular Ca2+
  • Covers detailed methodology plus problems and pitfalls of each technique
  • Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program
  • Color plates illustrate techniques such as
  • Confocal ratio-imaging
  • Use of aequorin


Researchers, graduate students, and technicians in cell, developmental, and molecular biology, neuroscience, physiology, biochemistry, biophysics, and optical sciences

Table of Contents

  • Ca2+ Buffers and [Ca2+]I Perturbation Techniques:
    D.M. Bers, C.W. Patton, and R. Nuccitelli, A Practical Guide to the Preparation of Ca2+ Buffers.
    R. Zucker, Photorelease Techniques for Raising or Lowering Intracellular Ca2+.
    D. Thomas and M.R. Hanley, Pharmacological Tools for Perturbing Intracellular Calcium Storage.
    Microelectrode Techniques for Measuring [Ca2+]I and Ca2+ Fluxes:
    S. Baudet, L. Hove-Madsen, and D.M. Bers, How to Make and to Use Calcium-Specific Mini- and Microelectrodes.
    P.J.S. Smith, R.H. Sanger, and L.F. Jaffe, The Vibrating Ca2+ Electrode: A New Technique for Detecting Plasma Membrane Regions of Ca2+ Influx and Efflux.
    C.A. Leech and G.G. Holz, IV, Application of Patch Clamp Methods to the Study of Calcium Currents and Calcium Channels.
    Fluorescence Techniques for Imaging [Ca2+]I:
    J.P.Y. Kao, Practical Aspects of Measuring [Ca2+] with Fluorescent Indicators.
    S.J. Morris, T.B. Wiegmann, L.W. Welling, and B.M. Chronwall, Rapid Simultaneous Estimation of Intracellular Calcium and pH.
    T.J. Keating and R.J. Cork, Improved Spatial Resolution in Ratio Images Using Computational Confocal Techniques.
    P.A. Diliberto, X.F. Wang, and B. Herman, Confocal Imaging of Ca2+ in Cells.
    S. Girard and D.E. Clapham, Simultaneous Near Ultraviolet and Visible Excitation Confocal Microscopy of Calcium Transients in Xenopus Oocytes.
    Use of Aequorin for [Ca2+]I Imaging:
    K.R. Robinson, T.J. Keating, and R. J. Cork, Inexpensive Techniques for Measuring [Ca2+]I Changes Using a Photomultiplier Tube.
    A.L. Miller, E. Karplus, and L.F. Jaffe, Imaging [Ca2+]I with Aequorin Using a Photon Imaging Detector.
    R. Rizzuto, M. Brini, and T. Pozzan, Targeting Recombinant Aequorin to Specific Intracellular Organelles.

Product details

  • No. of pages: 384
  • Language: English
  • Copyright: © Academic Press 1994
  • Published: March 10, 1994
  • Imprint: Academic Press
  • Paperback ISBN: 9780125228107
  • eBook ISBN: 9780080859385
  • Hardcover ISBN: 9780125641418

About the Serial Volume Editor

Richard Nuccitelli

Affiliations and Expertise

Division of Biological Sciences, University of California, Davis, U.S.A.

About the Serial Editors

Leslie Wilson

University of California, Santa Barbara, USA

Affiliations and Expertise

University of California, Santa Barbara, USA

Paul Matsudaira

Affiliations and Expertise

Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, U.S.A.

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