A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation.
Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes
Includes techniques for perturbing intracellular Ca2+
Covers detailed methodology plus problems and pitfalls of each technique
Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program
Color plates illustrate techniques such as
Use of aequorin
Researchers, graduate students, and technicians in cell, developmental, and molecular biology, neuroscience, physiology, biochemistry, biophysics, and optical sciences
Table of Contents
Ca2+ Buffers and [Ca2+]I Perturbation Techniques: D.M. Bers, C.W. Patton, and R. Nuccitelli, A Practical Guide to the Preparation of Ca2+ Buffers. R. Zucker, Photorelease Techniques for Raising or Lowering Intracellular Ca2+. D. Thomas and M.R. Hanley, Pharmacological Tools for Perturbing Intracellular Calcium Storage. Microelectrode Techniques for Measuring [Ca2+]I and Ca2+ Fluxes: S. Baudet, L. Hove-Madsen, and D.M. Bers, How to Make and to Use Calcium-Specific Mini- and Microelectrodes. P.J.S. Smith, R.H. Sanger, and L.F. Jaffe, The Vibrating Ca2+ Electrode: A New Technique for Detecting Plasma Membrane Regions of Ca2+ Influx and Efflux. C.A. Leech and G.G. Holz, IV, Application of Patch Clamp Methods to the Study of Calcium Currents and Calcium Channels. Fluorescence Techniques for Imaging [Ca2+]I: J.P.Y. Kao, Practical Aspects of Measuring [Ca2+] with Fluorescent Indicators. S.J. Morris, T.B. Wiegmann, L.W. Welling, and B.M. Chronwall, Rapid Simultaneous Estimation of Intracellular Calcium and pH. T.J. Keating and R.J. Cork, Improved Spatial Resolution in Ratio Images Using Computational Confocal Techniques. P.A. Diliberto, X.F. Wang, and B. Herman, Confocal Imaging of Ca2+ in Cells. S. Girard and D.E. Clapham, Simultaneous Near Ultraviolet and Visible Excitation Confocal Microscopy of Calcium Transients in Xenopus Oocytes. Use of Aequorin for [Ca2+]I Imaging: K.R. Robinson, T.J. Keating, and R. J. Cork, Inexpensive Techniques for Measuring [Ca2+]I Changes Using a Photomultiplier Tube. A.L. Miller, E. Karplus, and L.F. Jaffe, Imaging [Ca2+]I with Aequorin Using a Photon Imaging Detector. R. Rizzuto, M. Brini, and T. Pozzan, Targeting Recombinant Aequorin to Specific Intracellular Organelles. Index.