A Practical Guide to Protein and Peptide Purification for Microsequencing

2nd Edition

Print ISBN: 9780124802827
eBook ISBN: 9780080924618
Imprint: Academic Press
Published Date: 20th May 1993
Page Count: 184
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Why a Second Edition?
The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.

Key Features

  • Mass spectrophotometric characterization of proteins and peptides
  • N-terminal sequencing of proteins with blocked N termini
  • Internal amino acid sequence analysis after protease digestion in-gel and on-blot
  • Improved microscale peptide purification methods
  • Computer analysis of protein sequences
  • New protocols tested and refined through everyday use in authors' laboratories
  • Updated reference chapter covering all aspects of protein microsequencing


Researchers and students at all levels in cell, developmental, and molecular biology, protein biochemistry and biophysics, neurobiology, pharmacology, immunology, parasitology, virology, genetics, plant science, biotechnology, and other biomedical/biological areas

Table of Contents

Introduction. Strategies for Obtaining Partial Amino Acid Sequence Data from Small Quantities (5nmol) of Pure of Partially Purified Protein. Enzymatic Digestion of Proteins and HPLC Peptide Isolation. Purification of Proteins and Peptides By SDS-PAGE. Internal Amino Acid Sequence Analysis of Proteins After in Situ Protease Digestion on Nitrocellulose. Mass Spectrometric Strategies for the Structural Characterization of Proteins. Appendix. References. General Reference Books on Protein Microsequencing Methodologies.


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© Academic Press 1993
Academic Press
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Reviews of the First Edition:
"Like the evening network television news programs, this book gives a useful summary of the highlights (of protein chemistry) to enable the masses to go forth armed with enough information to survive in the world." --Cell