A Practical Guide to Protein and Peptide Purification for Microsequencing

A Practical Guide to Protein and Peptide Purification for Microsequencing

2nd Edition - May 20, 1993

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  • Editor: Paul Matsudaira
  • eBook ISBN: 9780080924618
  • Paperback ISBN: 9780124802827

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Why a Second Edition?The Second Edition provides practical answers to the general question, "How can I obtain useful sequence information from my protein or peptide?" rather than the more specific question asked in the first edition, "How can I obtain the N-terminal sequence?" Important new methods include ways of dealing with blocked N termini, computer analysis of protein sequences, and the recent revolution in mass spectrometry.

Key Features

  • Mass spectrophotometric characterization of proteins and peptides
  • N-terminal sequencing of proteins with blocked N termini
  • Internal amino acid sequence analysis after protease digestion in-gel and on-blot
  • Improved microscale peptide purification methods
  • Computer analysis of protein sequences
  • New protocols tested and refined through everyday use in authors' laboratories
  • Updated reference chapter covering all aspects of protein microsequencing


Researchers and students at all levels in cell, developmental, and molecular biology, protein biochemistry and biophysics, neurobiology, pharmacology, immunology, parasitology, virology, genetics, plant science, biotechnology, and other biomedical/biological areas

Table of Contents

  • Introduction. Strategies for Obtaining Partial Amino Acid Sequence Data from Small Quantities (5nmol) of Pure of Partially Purified Protein. Enzymatic Digestion of Proteins and HPLC Peptide Isolation. Purification of Proteins and Peptides By SDS-PAGE. Internal Amino Acid Sequence Analysis of Proteins After in Situ Protease Digestion on Nitrocellulose. Mass Spectrometric Strategies for the Structural Characterization of Proteins. Appendix. References. General Reference Books on Protein Microsequencing Methodologies.

Product details

  • No. of pages: 184
  • Language: English
  • Copyright: © Academic Press 2012
  • Published: May 20, 1993
  • Imprint: Academic Press
  • eBook ISBN: 9780080924618
  • Paperback ISBN: 9780124802827

About the Editor

Paul Matsudaira

Affiliations and Expertise

Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology, Cambridge, U.S.A.

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