A Manual of Paper Chromatography and Paper Electrophoresis

A Manual of Paper Chromatography and Paper Electrophoresis

2nd Edition - January 1, 1958

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  • Authors: Richard J. Block, Emmett L. Durrum, Gunter Zweig
  • eBook ISBN: 9781483276809

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A Manual of Paper Chromatography and Paper Electrophoresis provides a comprehensive discussion of the techniques of paper chromatography and paper electrophoresis. The book is organized into two parts. Part I on paper chromatography provides a readily accessible source for some of the many uses and adaptations of paper chromatography. An effort has been made to write a practical manual in which tried and proved procedures, employing relatively simple equipment and available reagents, are summarized. Part II on paper electrophoresis discusses basic principles and methodology. The emphasis throughout has been on the separation of protein mixtures, particularly blood serum. This reflects the fact that it is in this particular application that paper electrophoresis has thus far not been challenged by paper chromatography, whereas many of the smaller molecules can be resolved equally well or better by the thus far more widely employed chromatographic procedures.

Table of Contents

  • Preface

    Part I. Paper Chromatography

    I. Introduction

    II. Theory of Paper Chromatography

    III. General Methods

    IV. Quantitative Methods

    V. Amino Acids, Amines, and Proteins

    Section I: Amino Acids and Peptides

    Section II: Amino Acid Derivatives

    Section III: Amines

    Section IV: Separation of Proteins

    VI. Carbohydrates

    Section I: General Procedure

    Section II: Simple Sugars and Disaccharides

    Section III : Oligo- and Polysaccharides

    Section IV: Sugar Phosphates and Other Substituted Sugars

    Section V: Miscellaneous Derived Sugars

    VII. Aliphatic Acids

    Section I: Volatile Aliphatic Acids (C1 — C9)

    Section II: Non-Volatile Water-Soluble Aliphatic Acids

    Section III: α-Keto Acids

    Section IV: Higher Fatty Acids

    Section V: Miscellaneous Lipids

    VIII. Steroids and Bile Acids

    Section I: Steroids

    Section II: Bile Acids

    IX. Purines, Pyrimidines and Related Substances

    Section I: General Directions

    Section II: Detailed Experimental Procedures

    X. Phenols, Aromatic Acids, andPorphyrins

    Section I: Phenols and Aromatic Acids

    Section II: Porphyrins

    XI. Miscellaneous Organic Substances


    Ketones and Aldehydes

    Sulfa Drugs


    Adrenaline and Related Compounds


    Chlorogenic and Caffeic Acids

    Nicotinic Acid and Related Compounds

    Color Reagent for Isonicotinoyl Hydrazide

    Tryptophan and Indoleacetic Acid Derivatives


    Urea, Creatine, Creatinine, etc.

    Methylol Compounds


    Phospholipids and Their Nitrogenous Constituents

    Miscellaneous Organic Bases

    Chloroplast, Anthraquinone, and Other Pigments

    Coumarins and Related Compounds

    Flavonoid Pigments



    Flavin Derivatives

    Natural Pterins and Synthetic Derivatives

    pH Indicators and Other Dyes

    Coal Tar Colors

    Photographic Developers

    Organic Insecticides

    Polyamides (Nylons)

    Natural Rubber and Resins


    Guanidine Derivatives

    XII. Antibiotics and Vitamins

    Section I: Antibiotics

    Section II: Vitamins

    Section III: Plant Growth Substances

    XIII. Inorganic Separations

    Part II. Paper Electrophoresis


    XIV. General Theory


    Partition Chromatography



    XV. Methods

    General Considerations

    Closed Strip Method

    Open Strip Techniques

    Hanging Strip Method


    XVI. Two Dimensional Technique

    Closed Sheet Method

    Hanging Sheet Method

    Combination of Electrophoresis and Chromatography

    XVII. Continuous Electrophoresis

    XVIII. Some Quantitative Considerations


    Electrolytes Commonly Employed in Paper Electrophoresis




    Part I

    Part II

    Author Index

    Subject Index

Product details

  • No. of pages: 722
  • Language: English
  • Copyright: © Academic Press 1958
  • Published: January 1, 1958
  • Imprint: Academic Press
  • eBook ISBN: 9781483276809

About the Authors

Richard J. Block

Emmett L. Durrum

Gunter Zweig

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