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A Laboratory Guide to the Tight Junction offers broad coverage of the unique methods required to investigate its characteristics. The methods are described in detail, including its biochemical and biophysical principles, step-by-step process, data analysis, troubleshooting, and optimization. The coverage includes various cell, tissue, and animal models.
Chapter 1 provides the foundations of cell biology of tight junction. Chapter 2 covers the Biochemical approaches for paracellular channels and is followed by chapter 3 providing the Biophysical approaches. Chapter 4 describes and discusses Histological approaches for tissue fixation and preparation. Chapter 5 discusses Light microscopy, while chapter 6 presents Electron microscopic approaches. Chapter 7 covers Transgenic manipulation in cell cultures, including DNA and siRNA, Mutagenesis, and viral infection. Chapter 8 covers transgenic manipulation in mice, including: Knockout, Knockin, siRNA knockdown, GFP/LacZ reporter, and overexpression. The final chapter discusses the future developments of new approaches for tight junction research.
Researchers and advanced students in bioscience working on topics of cell junction, ion channel and membrane protein will benefit from the described methods. Clinicians and pathologists interested in tissue barrier diseases will also benefit from the biochemical and biophysical characterization of tight junctions in organ systems, and their connection to human diseases.
• Provides consistent and detailed research methods
• Covers various cell, tissue and animal models
• Includes step-by-step guidance from beginner to sophisticated levels
Researchers and advanced students in biomedical sciences, cell biology, physiology, pathology and pharmacology. Clinicians and pathologists interested in tissue barrier diseases
2. Biochemical approaches for tight junction
3. Biophysical approaches for tight junction
4. Fixation approaches for tight junction
5. Tissue preparation approaches for tight junction
6. Light microscopy for tight junction
7. Electron microscopy for tight junction
8. Transgenic manipulation of tight junction in cells
9. Transgenic manipulation of tight junction in mice
- No. of pages:
- © Academic Press 2020
- 2nd August 2020
- Academic Press
- Paperback ISBN:
Jianghui Hou is an Associate Professor of Medicine, Division of Nephrology, at Washington University Saint Louis. Hou received his bachelors in biochemistry from Nanjing University, China. He received Master (with distinction) and Ph.D. from the University of Edinburgh, UK. Dr. Hou did his postdoc in cell biology at Harvard Medical School. He has been at Washington University since 2009. Dr. Hou is a renowned expert in studying tight junctions in different tissues, has published a number of high-profile papers in this field, and has recently pioneered a novel approach for high-resolution measurement of the tight junction permeability.
Associate Professor of Medicine, Division of Nephrology, Washington University Saint Louis, USA