Please provide email address and ORCID for all authors. Please visit http://orcid.org/ for ORCID registration.
The main text and formal tables of manuscript should be prepared in Word file, justified and 1.5 spacing. Supplementary tables can be submitted in Excel format. Standard figure sizes are 85mm (single column) and 175mm (double column) for width vertically or 238 mm horizontally. Please adjust the font size for clarity. The figure format can be in ppt, ai(illustrator), jpeg. Please add line numbers in your manuscript and make sure the built PDF for submission has the corresponding line numbers too. There is no Abbreviation section, please spell the terms in full at first mention and put the abbreviation afterwards in parenthesis.

Title page

Article Title
[Insert article title here]
Note: We capitalize the first letter of each content word for the article title in the actual article and TOC. For preposition words, if there are more than 5 letters, the first letter should be in upper case too. Like “between” should be “Between” in the title. The title should be in bold and the font size should be 15. Align to the left.

Sample: 
The Association Between H3K4me3 and Antisense Transcription

Author(s) Names
[Insert author(s) names here]
[First Name][Middle Name] [Family Name] 1,
Note: There is a comma between two names, no “and” before the last author’s name. We prefer to use numbers instead of letters to label the authors’ institution as superscript. We prefer to use “*” to present the corresponding author(s), and use “#” to present the equal contribution author(s). 
Sample: 
Huanming Yang1,#, Donald J. Zack2,#, Songnian Hu1,*, Jun Yu1,*

Author(s) Address Information 
[Insert author(s) address here]
1 [Department/Institute], [University], [City] [Postal code], [Country] 
Sample:
1 Beijing Institute of Genomics, Chinese Academy of Sciences, Beijing 101300, China
2 Department of Molecular Biology and Genetics, Johns Hopkins University School of Medicine, Baltimore MD 21287, USA

Symbol
# Equal contribution.
* Corresponding author(s).
E-mail: [Corresponding author(s) E-mail] [Corresponding author(s) last name and initials of first and middle names] 
(with a comma between two authors)
Sample for E-mail:
E-mail: junyu@big.ac.cn (Yu J), husn@big.ac.cn (Hu S).

Running title:
Note: no more than 50 characters, italic; capitalize the initial letter of each content word
[The first author name] / [Running title].  No dots in between. If only two authors contributed to the work, then use both names rather than et al.
Sample:
Running title: Yang H et al / Correlation of H3K4me3 and Antisense Transcription
Running title: Mehrotra S and Goyal V / Repetitive Satellite DNA in Plants

Please also indicate the total counts of words, figures, tables, supplementary figures and supplementary tables.

Abstract page

Abstract
[Insert abstract here]
Note: This section should be in one paragraph, unstructured, less than 250 words.
Sample:
Abstract
Histone H3 lysine 4 trimethylation (H3K4me3) is well known to occur in the promoter region of genes for transcription activation. However, when investigating the H3K4me3 profiles in the mouse cerebrum and testis, we discovered that H3K4me3 also has a significant enrichment at the 3′ end of actively transcribed (sense) genes, named as 3′-H3K4me3. 3′-H3K4me3 is associated with ~15% of protein-coding genes in both tissues. Therefore, we speculate that these 3′-H3K4me3-marked regions may serve as potential promoters for antisense transcription and 3′-H3K4me3 appear to be a universal epigenetic feature in eukaryotes. Our results provide a novel insight into the epigenetic roles of H3K4me3 and the regulatory mechanism of antisense transcription.

KEYWORDS: [Insert key words here]; [Insert key words here]
Note: There should be 3−5 key words, with a semicolon between two key words, and capitalize the first letter of each keyword. Keyword used to extend the searchable possibilities for you manuscript, thus it is recommended to use words that did not appear in the article title. 
Sample:
KEYWORDS: Calcium transporter; Sorghum; Rice; Genomic annotation

Text body page 
Throughout the paper, please check the usage of protein and gene symbols, abbreviations, and names. Protein abbreviations/symbols are normally in Roman font (upright type), while the gene symbols/names should be in italics, normally in upper case for human genes (e.g., CMH1 and HPR) and in lower case with an initial capital for animal genes (viz. mouse; e.g., Hras1 and Tcra).

Introduction
[Insert introduction here]
Note: No indent before the 1st paragraph of a separate section is necessary. Use square bracket to show the reference number in the text [1]. And number the references according to the order of first appearance in the text.
The 1st paragraph should align to the left, with no indent. However, form the 2nd paragraph, there should be a 3-letter indent. 1.5 spaces are recommended throughout the whole manuscript.

Sample:
Introduction
The membrane-associated calcium transporter proteins are essential to maintain calcium homeostasis under normal physiological conditions and provide tolerance against various stresses. There are three major classes of calcium transporter proteins: channels, ATPases (pumps) and exchangers [1−6]. Members of these transporter proteins may differ in their cellular and tissue distribution and regulation by other signaling pathways [7,8]. 
The spatial and temporal regulation of calcium concentration in plant cells depends on the coordinated activities of channels and active transporters located on different organelles and membranes [9].

Results 
[Insert results here]
Note: The 1st-level title should be in the font size of 14 and in bold. The 2nd-level title should be in bold with the same font of the text (size 12). The 3-level title should be in italic with the same font of the text (size 12), not in bold. Only the first letter of a subtitle needs to be capitalized unless specific terms are used. 
The subtitles should be put in separate line from the following paragraphs.
All figures and tables should be numbered in the order of first mention. The name of specific figure or table should be put in bold at first mention. The name of supplementary figure or table does not need to be put in bold.
Sample:
Results
The 3′ end enrichment of H3K4me3 intervals
We aligned ChIP-seq reads to the 3′ end of these genes and observed that there is a considerable increase of H3K4me3 density around the 3′ end of 3′-H3K4me3-marked genes (Figure 1). The result of read profiling suggested that modified histones are concentrated both upstream and downstream of transcriptional termination sites (TTS), peaking at ~1 kb upstream of TTS. 
Correlation of H3K4me3 and antisense transcription
In the two mouse tissues examined, we identified 2,519 genes in total associated with 3′-H3K4me3, of which 31% (785 genes) are shared by both tissues while the remaining is specific to either cerebrum or testis (Figure 2A). The majority of the 785 shared genes are primarily involved in the regulation of transcription, metabolic processes and biosynthesis, which are all essential cellular functions (Table 1). In contrast, the tissue-specific genes are related to development. For example, 689 genes that show 3′-H3K4me3 in cerebrum only are associated with cerebrum-specific expression (Table S1).

Discussion
[Insert discussion here]
Note: This section can be combined with Results section.

Conclusion
[Insert conclusion here]
Note: This section is an optional section.

Materials and methods
[Insert materials and methods here]
Note: The format of this section should be consistent with previous sections.

Authors’ contributions 
[Insert authors’ contributions here]
Note: This section is a required section to indicate contribution from each author. And there is no dote “.” for author initials in this section.
Sample: 
Authors’ contributions 
AB carried out the molecular genetic studies, participated in the sequence alignment and drafted the manuscript. JY carried out the immunoassays. MT participated in the sequence alignment. ES participated in the design of the study and performed the statistical analysis. FG conceived of the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.

Competing interests
[Insert authors’ competing interests here]
Note: This section is a required section to declare any competing interest.
Sample: 
Competing interests
The authors have declared no competing interests.

Acknowledgements
[Insert acknowledgements here]
Note: Please acknowledge the funding agencies if available, provide the full name (including country name) of the program and grant No. Please also acknowledge people who help with the study or manuscript preparation.

Sample:
Acknowledgements
This work was supported by the National Institutes of Health of USA (Grant No. R01GM088342) awarded to YX. We thank Zhixiang Lu for testing PrimerSeq and performing RT-PCR experiments.

References
[Insert references here]

Note: 
The reference style for GPB is “Vancouver” system as shown below.
Text: Indicate references by number(s) in square brackets in line with the text. The actual authors can be referred to, but the reference number(s) must always be given. 
List: Number the references (numbers in square brackets) in the list in the order in which they appear in the text. 
Check Journals in NCBI Databases for the proper abbreviation of journal name (http://www.ncbi.nlm.nih.gov/nlmcatalog/journals).
Note shortened form for last page number. e.g., 51–9, 
For more than 6 authors, the first 6 should be listed followed by “et al.”
For further details you are referred to “Uniform Requirements for Manuscripts submitted to Biomedical Journals” (J Am Med Assoc 1997;277:927–34), see also http://www.nlm.nih.gov/tsd/serials/terms_cond.html 
Please make sure all the species names are put in italics in the reference list. 
The template for Endnote output style used in GPB can be downloaded at http://gpb.big.ac.cn.

Samples:
Reference to a journal publication: 
[1] Van der Geer J, Hanraads JAJ, Lupton RA. The art of writing a scientific article. J Sci Commun 2000; 163: 51−9. 
[2] Zheng L, Foley K, Huang L, Leubner A, Mo G, Olino K, et al. Tyrosine 23 phosphorylation-dependent cell-surface localization of annexin A2 is required for invasion and metastases of pancreatic cancer. PLoS One 2011; 6: e19390.
Reference to a book: 
[3] Strunk Jr W, White EB. The elements of style. 3rd ed. New York: Macmillan; 1979. 
Reference to a chapter in an edited book: 
[4] Mettam GR, Adams LB. How to prepare an electronic version of your article. In: Jones BS, Smith RZ, editors. Introduction to the electronic age. New York: E- Publishing Inc; 1999, p. 281−304. 
Reference to conference proceeding / abstract: 
[5] Yasuda N, Takagi S-i, Toriumi A. Spectral shape analysis of infrared absorption of thermally grown silicon dioxide films. Appl Surf Sci 1997;117−118 (June (II)):216−20.
Reference to an ebook or online PDF: 
[1] Smith K. A brief history of NCBI’s formation and growth. In: The NCBI handbook [Internet]. 2nd ed. Bethesda: National Center for Biotechnology Information; 2013, http://www.ncbi.nlm.nih.gov/books/NBK148949/. 
[2] National Research Council (US) Committee on Mapping and Sequencing the Human Genome. Mapping and sequencing the human genome. Washington DC: National Academies Press; 1988, http://www.ncbi.nlm.nih.gov/books/NBK218252/.
[3] US Congress, Office of Technology Assessment. Mapping our genes – the Genome Project: how big, how fast? Washington, DC: US Government Printing Office;1988,  http://www.ornl.gov/sci/techresources/Human_Genome/publicat/OTAreport.pdf
Reference to an ebook or online pdf:
[4] Smith K. A brief history of NCBI’s formation and growth. In: The NCBI handbook [Internet]. Bethesda: National Center for Biotechnology Information; 2013, http://www.ncbi.nlm.nih.gov/books/NBK148949/ 
Please do not put webpage links into the reference section. The links can be added in a parenthesis right after the citing materials in the main text.
Figure legends
[Insert figure legends here]
Note: In this section, please include figure titles, figure legends and table titles. “Figure legends” should be in bold in 14-point font. We put figure titles (12-point font) in bold. And we spell out “Figure” in the title line (no abbreviation of “fig”). The figure title should be in the same line with the figure number (two spaces in between). No full stop dot "." at the end of the figure titles.   
The legends should be in regular fonts in separate lines and in the legends, only the panel index labelling (A, B, C...) is in bold. And there is a full stop dot “.” at the end of the legend.
Samples: 
Figure legends
Figure 1  Comparison of genes with 3′-H3K4me3 between mouse cerebrum and testis 
A. Numbers of tissue-specific genes modified with 3′-H3K4me3 and genes shared by both cerebrum and testis. There are 785 genes commonly modified in both tissues, 689 uniquely-modified genes in the cerebrum, and 1,045 uniquely-modified genes in the testis. B. The Wnt1 and Stox1 genes, as examples, show tissue-specific 3′ H3K4me3. We examined the densities of H3K4me3 (red) and pan-H3 (green) for both genes.
Figure 2  H3K4me3 and H3K27me3 modifications around TSS 
Profiles of H3K4me3 modifications across the TSS were shown for high, medium, low, ambiguous, and silent expression genes in cerebrum (A) and testis (B). Profiles of H3K27me3 modifications across the TSS were shown for high, medium, low, ambiguous, and silent expression genes in cerebrum (C) and testis (D).

Tables
[Insert table legends here]
Note: The table title (12-point font) should be in bold and in the same line with the table number (two spaces in between). No full stop dot "." at the end of the table titles.   

Sample
Table 1  Pathway classification of genes based on KEGG analysis
Supplementary material
[Insert supplementary material legend here]
Note: In this section, please include supplementary figure titles, figure legends and table titles as you do for the Figure legends section. You can also include the title for supplementary file (including file name, file format, name and URL link of appropriate viewer if format is unusual.)

Files for figures and tables
Figures and tables
Note: Figures and tables should not be embedded in the main text; instead upload them separately, 1 figure/table in 1 file.

Tables
Note: We use three-line tables and put table titles in bold. The table title should be in the same line with the table number (two spaces in between). No full stop dot "." at the end of the table titles.   
The table header should be put in bold. Footnote can be added under the table for explanation. And there is a full stop dot “.” at the end of the note. Only the word “Note:” should be italic.
Sample:
Table 1  Pathway classification of genes based on KEGG analysis
Category	Mapped contigs	Singletons	Total	%	
Metabolism	114	109	230	28.01	
Human diseases	68	75	143	17.41	
Note: Percentage of enriched pathways in buffalo muscle tissue was calculated after analysis with KEGG pathway. 

Figures
Note: Please put the panel index on the upper left corner of the respective panel; asterisk could be used for significance and no terms (e.g. P < 0.05) is needed in the figure; For the limited space, please provide the measurement unit in the end of the axis terms (ng/ml).
Figures should be of high resolution. Please use “Arial” for font style. In all text boxes, only the first letter of the first word should be put in upper case, unless specific terms are used. Axis titles should be provided for all graphs; legend box should be put within the plotting area whenever possible without borders. Standard figure sizes are 85mm (single column) and 175mm (double column) for width vertically or 238 mm horizontally. Please adjust the font size for clarity. Please provide the editable version if possible.
If you adopt or modify the figure from those published previously, please get the permission from the original authors or journals.