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 Principles and Practice
To order this title, and for more information, click here
Third Edition
By
James Goding, Monash University, Alfred Hospital, Prahran, Victoria, Australia
Description
Monoclonal Antibodies now have applications in virtually all areas of biology and medicine, and much of the world's biotechnology
industry has its foundations in the exploitation of this technology.
The Third Edition of this well established book meets the needs
of both newcomers to the area and experienced researchers, by providing an integrated treatment of both the production and application
of monoclonal antibodies. As in previous editions, detailed and critical accounts of the theory, production, purification, fragmentation,
storage and radiolabelling of monoclonal antibodies are given, along with descriptions of their use in antigen characterization, affinity
chromatography and immunofluorescence. The present volume has been comprehensively updated to cover recent rapid advances, particularly
with respect to the applications of molecular biology, the use of antibodies in closing and heterologous expression of genes, immunohistology
and phage display libraries. Since the previous edition, there has been a growing trend towards the replacement of procedures using radioactive
isotopes, and the current edition incorporates these newer technologies.
The text is oriented towards problems solving, and makes it
easy to adapt each procedure to individual needs. Extensive cross-referencing, a glossary and a comprehensive index make this book an
essential reference.
This book will be vital both for laboratories already producing or using monoclonal antibodies, and for workers
in many disciplines who are contemplating their use.
Contents
Introduction. The Antibody Response. Cellular Basis of the Immune System. Nature of Antigens. Antibody Structure and Function. Genetics
of Antibodies. Introduction to Monoclonal Antibodies. Production of Monoclonal Antibodies. Purification. Fragmentation and Isotopic Labeling
of Monoclonal Antibodies. Analysis of Antigens Recognized By Monoclonal Antibodies. Affinity Chromatography Immunofluorescence. Immunohistology.
Construction, Screening and Expression of Recombinant Antibodies. Generation of Conventional Antibodies.
Bibliographic & ordering Information
Hardbound, 492 pages, publication date: FEB-1996
ISBN-13: 978-0-12-287023-1
ISBN-10: 0-12-287023-9
Imprint: ACADEMIC PRESS
Price: Order form
USD 97.95
EUR 91.95
GBP 62.99
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Last update: 29 Aug 2008
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