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| MOLECULAR MOTORS AND THE CYTOSKELETON, PART B, 298
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 Volume 298
To order this title, and for more information, click here
Richard Vallee, Worcester Foundation for Experimental Biology, Shrewsbury, Massachusetts, U.S.A.
John Abelson, California Institute of Technology, Division of Biology, Pasadena, U.S.A.
Melvin Simon, California Institute of Technology, Division of Biology, Pasadena, USA
Description
General Description of the Volume:
This Methods in Enzymology volume continues the description (initiated in Volume 85)
of the purification and assay of this diverse group of proteins involved in cytoplasmic organization. It discusses some of the more promising
attempts to apply techniques of molecular biology genetics and molecular cytochemistry to these proteins. General Description of
the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most
highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and
praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material
still relevant today--truly an essential publication for researchers in all fields of life sciences.
Contents
Analysis of Actomyosin-Related Systems:
R.E. Cheney, Purification and Assay of Myosin V.K. Ayscough, Use of Latrunculin-A,
An Actin Monomer Binding Drug.M.R. Bubb and I. Spector, Use of the F-Actin Binding Drugs, Misakinolide A and Swinholide A.E.J.
Luna, F-Actin Blot Overlays.J.F. Kelleker, R.D. Mullins, and T.D. Pollard, Purification and Assay of the Arp2/3 Complex from Acanthamoeba castellani.M.D. Welch and T.J. Mitchison, Purification and Assay of the Platelet Arp2/3 Complex.K.L.
Schmeichel, B.E. Stronach, and M.C. Beckerle, Purification and Assay of Zyxin.C.E. Turner and M.C. Brown, Purification and
Assays for Paxillin.J. Lacoste, A. Ma, and J.T. Parsons, Assay and Purification of Focal Adhesion Kinase.T. Jarchau, T. Mund,
M. Reinhard, and U. Walter, Purification and Assays of VASP.J.A. Theriot and D.C. Fung, Listeria monocytogenes-based
Assays for Actin Assembly Factors.
Analysis of Microtubule-Related Systems:
A. Desai and T.J. Mitchison, Preparation and
Characterization of Caged Fluorescein Tubulin.D. Meyer, D.R. Rines, A. Kashina, and J.M. Scholey, Purification of Novel Kinesins
from Embryonic Systems.D.W. Pierce and R.D. Vale, Assaying Processive Movement of Kinesin by Fluorescence Microscopy.J.B.
Bingham, S.J. King, and T.A. Schroer, Purification of Dynactin and Dynein from Brain Tissue.M.P. Sheetz and J. Kumar, Isolation
and Characterization of Kinectin.G.S. Diamantopoulos, T.E. Kreis, and J.E. Rickard, Purification and Assay of CLIP-170.F.
McNally, Purification and Assay of the Microtubule-Severing Protein, Katanin.Y. Zheng, M.L. Wong, B. Alberts, and T. Mitchison,
Purification and Assay of gTubulin Ring Complex.M. Blomberg-Wirschell and S.J. Doxsey, Rapid Isolation of Centrosomes.G.A.
Orr, S. Rao, C.S. Swindell, D.G.I. Kingston, and S.B. Horwitz, Photoaffinity Labeling Approach to Map Taxol-Binding Site on the Microtubule.
M.A. Jordan and L.Wilson, Use of Drugs to Study Role of Microtubule Assembly Dynamics in Living Cells.
Other Cytoskeletal Systems:
K. Oegema, A. Desai, M.L. Wong, T.J. Mitchison, and C.M. Field, Purification and Assay of A Septin Complex from Drosophila
Embryos.A. Mukherjee and J. Lutkenhaus, Purification, Assembly and Localization of FtsZ.C. Lu and H.P. Erickson, Purification
and Assembly of FtsZ.
Cell-Free and Genetic Systems:
E.D. Salmon, E. Yeh, S. Shaw, B. Skibbens, and K. Bloom, High Resolution
Video and Digital Enhanced Differential Interference Contrast Light Microscopy of Cell Division in Budding Yeast.D.A. Compton,
Production of M-phase and I-phase Extracts from Mammalian Cells.V.J. Allan, Organelle Motility and Membrane Network Formation
in Metaphase and Interphase Cell-Free Extracts.C. McGoldrick and M. Sheetz, Organelle Motility and Membrane Network Formation
from Cultured Mammalian Cells.S.L. Rogers, I.S. Tint, and V.I. Gelfand, An in Vitro Motility Assay for Melanophore Pigment
Organelles.J.K. Burkhardt, Purification of Phagosomes and Assays for Microtubule Binding.J. Scheel and T.E. Kreis, Magnetic
Bead Assay for Characterization of Microtubule-Membrane Interactions.L. Haimo, Reactivation of Vesicle Transport in Lysed Teleost
Melanophores.M.P. Koonce, U. Euteneuer, and M. Schliwa, Reactivatable Cell Models of Giant Ameba, Reticulomyxa.
Force
Production Assays:
R.J. Baskin, Design and Use of the Centrifuge Microscope to Assay Force Production.Y.J. Han and J.R.
Sellers, Motility Assays on Molluscan Native Thick Filaments.A.D. Mehta, J.T. Finer, and J.A. Spudich, Use of Optical Traps
in Single Molecule Study on Nonprocessive Biological Motors.K. Visscher and S.M. Block, Versatile Optical Traps with Feedback
Control.Y. Wang and R.J. Pelham, Jr., Preparation of a Flexible, Porous Polyacrylamide Substrate for Mechanical Studies of Cultured
Cells.T. Oliver, K. Jacobson, and M. Dembo, Design and Use of Substrata to Measure Traction Forces Exerted by Cultured Cells.
General Methods:
C.M. Field, K. Oegema, Y. Zheng, T.J. Mitchison, and C.E. Walczak, Purification of Cytoskeletal Proteins
Using Peptide Antibodies.J.E. Eriksson, D.M. Toivola, C. Sahlgren, A. Mikhailov, and A.S. Harmala-Brasken, Strategies to Assess
Phosphoprotein Phosphatase and Protein Kinase-Mediated Regulation of the Cytoskeleton.T.M. Svitkina and G.G. Borisy, Correlative
Light and Electron Microscopy of the Cytoskeleton of Cultured Cells.
Subject Index.
Author Index.
Bibliographic & ordering Information
Hardbound, 636 pages, publication date: AUG-1998
ISBN-13: 978-0-12-182199-9
ISBN-10: 0-12-182199-4
Imprint: ACADEMIC PRESS
Price: Order form
USD 175
GBP 88
EUR 128
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Last update: 30 Aug 2008
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