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GUIDE TO PROTEIN PURIFICATION, 182
Guide to Protein Purification, 182
Volume 182: Guide to Protein Purification
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John Abelson, California Institute of Technology, Division of Biology, Pasadena, U.S.A.
Murray Deutscher, University of Connecticut Health Center, Farmington, Connecticut, U.S.A.
Melvin Simon, California Institute of Technology, Division of Biology, Pasadena, USA

Included in series
Methods in Enzymology,

Description
Guide to Protein Purification, designed to serve the needs of the student, experienced researcher and newcomer to the field, is a comprehensive manual that provides all the up-to-date procedures necessary for purifying, characterizing, and handling proteins and enzymes in one source.

Audience
Protein biochemists, enzymologists, analytical biochemists, graduate students, and postdoctorates in these disciplines, cell and molecular biologists, biotechnologists, and industrial researchers working with protein products.

Contents
(Section Headings): Developing Purification Procedures. General Methods for Handling Proteins and Enzymes. Optimization of Starting Materials. Preparation of Extracts and Subcellular Fractionation. Solubilization Procedures. Purification Procedures: Bulk Methods. Purification Procedures: Chromatographic Methods. Purification Procedures: Electrophoretic Methods. Specialized Purification Procedures. Characterization of Purified Proteins. Immunological Procedures. Additional Techniques. Concluding Remarks. Appendix: Personal Perspectives on Enzyme Purification. Author Index. Subject Index.

Bibliographic details
Hardbound, 894 pages, publication date: FEB-1990
ISBN-13: 978-0-12-182083-1
ISBN-10: 0-12-182083-1
Imprint: ACADEMIC PRESS

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Last update: 30 Nov 2009
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