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A Practical Guide to Technology for Quantitative Real-Time Analysis
To order this title, and for more information, click here
Second Edition
Edited By
W. Mason, Cambridge, U.K.
Included in series
Biological Techniques Series,
Description
The use of fluorescent and luminescent probes to measure biological function has increased dramatically since publication of the First
Edition due to their improved speed, safety, and power of analytical approach. This eagerly awaited Second Edition, also edited by Bill
Mason, contains 19 new chapters and over two thirds new material, and is a must for all life scientists using optical probes.
The contents
include discussion of new optical methodologies for detection of proteins, DNA and other molecules, as well as probes for ions, receptors,
cellular components, and gene expression. Emerging and advanced technologies for probe detection such as confocal laser scanning microscopy
are also covered. This book will be essential for those embarking on work in the field or using new methods to enhance their research.
Audience
Cell biologists, biochemists, molecular biologists, and physiologists; postgraduates and research workers in both academia and industry
and clinical researchers. This book will be invaluable for those wishing to start using optical probes and also for researchers in the
field who want to use the most up-to-date techniques.
Contents
Part 1: Introduction to Fluorescence Microscopy:
J.S. Ploem, Fluorescence Microscopy.
Part II: Optical
Probes and Their Applications:
F.H. Kasten, Introduction to Fluorescent Probes: Properties, History, and Applications.
R.P. Haugland and I.D. Johnson, Intracellular Ion Indicators. V.L. Singer and R.P. Haugland, Fluorescent Imaging of
Nucleic Acids and Protein in Gels.
Part III: Using Optical Probes in Cells - Practicalities, Problems, and Pitfalls:
D.N. Bowser, S.H. Cody, P.N. Dubbin, and D.A. Williams, Introducing and Calibrating Fluorescent Probes in Cells and Organelles.
F. Bobanovic, Electroporation: A Method for Introduction of Non-permeable Molecular Probes.S.L. Shorte and S. Bolsover,
Imaging Reality: Understanding Maps of Physiological Cell Signals Measured by Fluorescence Microscopy and Digital Imaging.J. Hoyland,
Fluorescent Probes in Practice - Potential Artifacts.
Part IV: Optical Probes for Specific Molecules, Organelles, and Cells:
A.V. Zelenin, Acridine Orange as a Probe for Molecular and Cell Biology.J.W. Kok and D. Hoekstra, Fluorescent Lipid
Analogues: Applications in Cell and Membrane Biology.S.R. Adams, B.J. Bacskai, S.S. Taylor and R.Y. Tsien, Optical Probes for
Cyclic AMP.
Part V: Technology for Qulaitative and Quantitative Detection of Optical Probes in Living Cells:
W.T.
Mason, J. Dempster, J. Hoyland, T.J. McCann, B. Somasundaram and W. O'Brien, Quantitative Digital Imaging of Biological Activity
in Living Cells with Ion-sensitive Fluorescent Probes.J. Dempster, Fast Photomeric Measurements of Cell Function.L.M.
Loew, Potentiometric Membrane Dyes and Imaging Membrane Potential in Single Cells.J.-y. Wu, L.B. Cohen, and C.X. Falk,
Fast Multisite Optical Measurement of Membrane Potential, with Two Examples.S. Antic and D. Zecevic, Imagine Membrane Potential
Changes in Individual Neurons.
Part VI: Using Novel Indications for Genetic, Molecular, and Cellular Function:
G.B.
Sala-Newby, J.M. Kendall, Helen E. Jones, Kathryn M. Taylor, Michael N. Badminton, David H. Llewellyn and A.K. Campbell, Bioluminescent
and Chemiluminescent Indicators for Molecular Signaling and Function in Living Cells.G.A. Rutter, A.A. Culbert and J.M. Tavare,
Luminescence Imaging of Gene Expression in Single Living Cells.S.R. Kain, Enhanced Variants of the Green Fluorescent Protein
for Greater Sensitivity, Different Colors, and Detection of Apoptosis.R. Haggart, N. Bodsworth, and N. Foote, Rapid Detection
of Microorganisms.
Part VII: Applications of Confocal Microscopy for Optical Probe Imaging:
C.J.R. Sheppard,
Confocal Microscopy - Principles, Practice, and Options.K.-P. Yip and I. Kurtz, Dual-excitation Confocal Fluorescence Microscopy.
Y.S. Prakash, M.S. Kannan, and G.C. Sieck, High-speed Confocal Imaging in Four Dimensions.G. Cox and C. Sheppard,
Multiphoton Fluorescence Microscopy.P. Lipp and M.D. Bootman, High-resolution Confocal Imaging of Elementary Ca2+ Signals in
Living Cells.A. Ichihara, T. Tanaami, H. Ishida, and M. Shimizu, Confocal Fluorescent Microscopy Using Nipkow Scanner.S.H.
Cody and D.A. Williams, Optimizing Confical Microscopy for Thick Biological Specimens.B.R. Masters and B. Chance, Redox
Confocal Imaging: Intrinsic Fluorescent Probes of Cellular Metabolism.
Part VIII: Advanced Imaging and Light Detection Approaches
for Optical Probe Applications:
G.J. Puppels, Confocal Raman Microspectroscopy.C. Rothmann, I. Bar-Am, Z. Dubinsky,
S. Katz, and Z. Malik, Spectral Imaging of Autofluorescence Molecules and DNA Probes.B. Masters, P.T.C. So, and E. Gratton,
Multiphoton Excitation Microscopy and Spectroscopy of Cells, Tissues, and Human Skin In Vivo.R. Wolthuis, T.C. Bakker Schut,
P.J. Caspers, H.P.J. Buschman, T.J. R~mer, H.A. Bruining, and G.J. Puppels, Raman Spectroscopic Methods for In Vitro and In Vivo Tissue Characterization.G.J. Puppels, J.M.C.C. Coremans, and H.A. Bruining, In Vivo Semiquantitative NADH-fluorescence
Imaging.T.W.J. Gadella Jr., Fluorescence Lifetime Imaging Microscopy (FLIM): Instrumentation and Applications.G. Skews
and T. McCann, Detection of Fluorescently Labelled Proteins following Gel Electrophoresis: A Key Enabling Technology for Proteomics.
Part IX: CCD Cameras: Key Enabling Technologies for Optical Probe Imaging:
P. Tomkins and A. Lyons, Properties
of Low-light-level Intensified Cameras.R. Aikens, Properties of Low-light-level Slow-scan Detectors.C.D. Mackay,
High-speed Digital CCD Cameras - Principles and Applications.
Part X: Flow Cytomeric Methodologies for Measurement of Optical
Probes in Living Cells:
E.R. Simons, Flow Cytometry: Use of Multiparameter Kinetics to Evaluate Several Activation
Parameters Simultaneously in Individual Living Cells.A.M. Gurney, Photolabile Caged Compounds.K. Hahn, J. Kolega, J. Montibeller,
R. DeBasio, P. Post, J. Myers and D.L. Taylor, Fluorescent Analogues: Optical Biosensors of the Chemical and Molecular Dynamics
of Macromolecules in Living Cells.
Part XI: Applications of Optical Probe Imaging to Biological Problems:
M. Fricker,
C. Plieth, H. Knight, E. Blancaflor, M.R. Knight, N.S. White, S. Gilroy, Fluorescence and Luminescence Techniques to Probe Ion Activities
in Living Plant Cells.G. Shamkar and M.A. Horton, Nuclear Calcium: Concepts and Controversies.A.W. de Feijter, J.E. Trosko,
and M.H. Wade, Assessment of Gap Junctional Intercellular Communications in Living Cells Using Fluorescence Techniques.K.E.
Sawin, J.A. Theriot and T.J. Mitchison, Photoactivation of Fluorescence as a Probe for Cytoskeletal Dynamics in Mitosis and Cell
Motility.K. Florine-Casteel, J.L. Lemasters and B. Herman, Video Imaging of Lipid Order.
Index.
| Bibliographic details |
Spiral Bound, 647 pages, publication date: APR-1999
ISBN-13: 978-0-12-447836-7
ISBN-10: 0-12-447836-0
Imprint: ACADEMIC PRESS
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