Description Chemiluminescence immunoassay is now established as one of the best alternatives to conventional radioimmunoassay for the quantitation
of low concentrations of analytes in complex samples. During the last two decades the technology has evolved into analytical procedures
whose performance far exceeds that of immunoassays based on the use of radioactive labels. Without the constraints of radioactivity,
the scope of this type of analytical procedure has widened beyond the confines of the specialist clinical chemistry laboratory to other
disciplines such as microbiology, veterinary medicine, agriculture, food and environmental testing. This is the first work to present
the topic as a subject in its own right.
In order to provide a complete picture of the subject, overviews are presented of the individual
areas of chemiluminescence and immunoassay with particular emphasis on the requirements for interfacing chemiluminescent and immunochemical
reactions. The possible ways of configuring chemiluminescence immunoassays are described. State-of-the-art chemiluminescence immunoassay
systems are covered in detail together with those systems which are commercially available.
The book is aimed at researchers and
routine laboratory staff in the life sciences who wish to make use of this high-performance analytical technique and also at those interested
in industrial applications of the technology in the food, agricultural and environmental sciences.
Contents
1. Introduction. 2. Chemiluminescence: The Phenomenon. Photochemical and photophysical processes. Luminescence. Chemiluminescence in vivo: bioluminescence. Chemiluminescence in vitro. Mechanistic aspects. Measurement.
3. Immunoassay.
Historical. Labelled-antigen and labelled-antibody techniques. Radioactive and non-radioactive labels. Immunoassay design. The influence
of the label on the choice of architecture.
4. The Immunochemical/Photochemical Interface. Suitable chemiluminescent
molecules. Direct coupling: potential chemistries. Indirect coupling. The potential of bioluminescent systems.
5. Chemiluminescence
Immunoassays: The Early Work. The luminol experience. Isoluminol derivatives. Indirect chemiluminescence immunoassays. Immunoassays
for small molecules. Immunoassays for large molecules. Enzyme mediated systems.
6. Homogeneous Immunoassays. Monitoring
changes in kinetics and intensity. Monitoring changes in wavelength. Examples of homogeneous chemiluminescence immunoassays.
7.
Chemiluminescence Immunoassays: State of the Art. Indirect systems. Phthalhydrazide labels. Acridinium labels. Practical aspects.
8. Future Prospects. Future developments in chemiluminescence immunoassay. The impact on the clinical laboratory. The
impact in other areas of analysis. Conclusion. References. Appendix I. Appendix II. Subject index.
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