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GUIDE TO PROTEIN PURIFICATION, 463
Guide to Protein Purification, 463
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Second Edition

Edited By
Richard Burgess
Murray Deutscher, University of Connecticut Health Center, Farmington, Connecticut, U.S.A.

Included in series
Methods in Enzymology,

Contents


  1. Why Purify Enzymes?
  2. Strategies and Considerations for Protein Purifications (Linn)
  3. Bioinformatics in Planning a Protein Purification (Burgess)
  4. Preparing a Protein Purification Summary Table (Burgess)
  5. Setting up a Laboratory (Deutscher)
  6. Buffers: Principle and Practice (Blanchard)
  7. Measurement of Enzyme Activity (Harris)
  8. Quantitation of Protein (Bailey and Noble)
  9. Maintaining Protein Stability (Deutscher)
  10. Strategy for Choosing and Expression System (Brondyk)
  11. Bacterial Expression Systems (Collart)
  12. Yeast Expression Systems (Cregg)
  13. Baculovirus-Insect Cell Expression Systems (Jarvis)
  14. Mammalian Expression Systems (Geisse and Knopf)
  15. Expression of Tagged Proteins (Melhorta)
  16. Re-folding of Solubilized Inclusion Body Proetin (Burgess)
  17. Preparation of Biological Extract (Grabski)


Bibliographic details
Paperback, 912 pages, publication date: NOV-2009
ISBN-13: 978-0-12-374978-9
Imprint: ACADEMIC PRESS

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Last update: 9 Nov 2009
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