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BIOCONJUGATE TECHNIQUES
Bioconjugate Techniques
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Second Edition

By
Greg Hermanson, Director of Technology, Thermo Fisher Scientific, Rockford, IL USA

Audience
Researchers in biochemistry, molecular biology, immunology, pharmacology, cell biology, and microbiology

Contents
PART I Bioconjugate Chemistry 1. Functional Targets 2. The Chemistry of Reactive Groups PART II Bioconjugate Reagents 3. Zero-Length Cross-Linkers 4. Homobifunctional Cross-linkers 5. Heterobifunctional Cross-linkers 6. Trifunctional Cross-linkers 7. Dendrimers and Dendrons 8. Cleavable Reagent Systems 9. Fluorescent Probes 10. Bifunctional Chelating Agents and Radioimmunoconjugates 11. Biotinylation Reagents 12. Iodination Reagents 13. Silane Coupling Agents 14. Microparticles and Nanoparticles 15. Buckyballs, Fullerenes, and Carbon Nanotubes 16. Mass Tags and Isotope Tags 17. Chemoselective Ligation; Bioorthogonal Reagents 18. Discrete PEG Compounds PART III Bioconjugate Applications 19. Preparation of Hapten - Carrier Immunogen Conjugates 20. Antibody Modification and Conjugation 21. Immunotoxin Conjugation Techniques 22. Preparation of Liposome Conjugates and Derivatives 23. Avidin - Biotin Systems 24. Preparation of Colloidal-Gold-Labeled Proteins 25. Modification with Synthetic Polymers 26. Enzyme Modification and Conjugation 27. Nucleic Acid and Oligonucleotide Modification and Conjugation 28. Bioconjugation for the Study of Protein Interactions

Bibliographic details
Hardbound, 1323 pages, publication date: APR-2008
ISBN-13: 978-0-12-370501-3
ISBN-10: 0-12-370501-0
Imprint: ACADEMIC PRESS

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Last update: 11 Nov 2009
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