Edited by
Irina Klimanskaya, Advance Cell Technology, Worcester, MA, USA
Robert Lanza, Chief Scientific Officer, Advanced Cell Technology, MA, USA
Adjunct Professor, Institute of Regenerative Medicine, Wake Forest University School of Medicine, NC, USA
Description
This is the second of three planned volumes in the
Methods in Enzymology series on the topic of stem cells. This volume
is a unique anthology of stem cell techniques focusing on adult stem cells, and written by experts from the top laboratories in the world.
The contributors not only have hands-on experience in the field but often have developed the original approaches that they share with
great attention to detail. The chapters provide a brief review of each field followed by a “cookbook” and handy illustrations. The collection
of protocols includes the isolation and maintenance of stem cells from various species using “conventional” and novel methods, such as
derivation of ES cells from single blastomeres, differentiation of stem cells into specific tissue types, isolation and maintenance of
somatic stem cells, stem cell-specific techniques and approaches to tissue engineering using stem cell derivatives. The reader will find
that some of the topics are covered by more than one group of authors and complement each other. Comprehensive step-by-step protocols
and informative illustrations can be easily followed by even the least experienced researchers in the field, and allow the setup and
troubleshooting of these state-of-the-art technologies in other laboratories.
Included in series
Methods in Enzymology
Audience:
Biochemists, cell and molecular biologists, and bioengineers working with stem cells.
