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| PROTEIN BIOCHEMISTRY AND PROTEOMICS
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By
Hubert Rehm
Description
Hubert Rehm's Protein Biochemistry and Proteomics is more than a laboratory manual; it is a strategic guide that provides
the reader with tips and tricks for more successful lab experiments. Using a conversational yet professional tone, Rehm provides an overview
of a variety of methods in protein biochemistry/proteomics. He provides short and precise summaries of routine procedures as well as
listings of the advantages and disadvantages of alternative methods. Readers will immediately sense that the author if very familiar
with the challenges, and frustration of the daily lab routine. Never before has such an honest, tactical guide been available for those
conducting lab experiments within the field of biochemistry.
Audience
Laboratory practitioners such as students, instructors, and professionals, conducting experiments within the field of protein biochemistry.
Contents
The Concept of the Experimenter
Abbreviations
1. The Daily Bread
1.1 Making Buffers
1.2 Protein Determination
1.3 Gels
1.4 Staining
Gels
1.5 Precipitate and Concentrating
1.6 Blotting
1.7 Autoradiography of Gels and Blots
2. Ligand Binding
2.1 Radioactive Ligand
Marking
2.2 Binding
2.3 Analysis of Binding Data
2.4 Crosslinking of Ligands
2.5 Purposes
3. Solubilization of Membrane Proteins
3.1 Detergents
3.2 Solubilization
4. Protein Detection via Functional Measurements
4.1 Translocators
4.2 Reconstitution
4.3 Flux
Assay
4.4 Constructive Thoughts
5. Cleaning and Purifying
5.1 Pure Fun
5.2 Conventional Purification Methods
5.3 Affinity Chromatography
5.4 The Purity Test
5.5 Profiting
6. Antibodies
6.1 Production of Polyclonal Antibodies
6.2 Immunoprecipitation
6.3 Immunoaffinity
Chromatography
6.4 Antibodies Against Unpurified Proteins
6.5 Immunological detection techniques
7. Proteomics
7.1 Introduction
7.2 Sample taking
7.3 2D gel electrophoresis
7.4 Mass Spectroscopy of Peptides and Proteins
7.5 Protein Chips
7.6 Microsequencing
7.7 Strategy
8. Subunits
8.1 Number & Stoichiometry of Subunits
8.2 What Holds Our World Together
9. Glycoproteins
9.1 How, Where
and for What Purpose are Proteins Glycosylated?
9.2 Detecting Glycoproteins in Gels
9.3 Detection of Glycoproteins on Blots
9.4 Deglycosylation
9.5 The Sugar Chains
10. Treasure Island
10.1 Of the Paper
10.2 Of Writing a Paper
11. Desert Planet
12. Jaws
12.1 Suppliers
12.2
Suppliers by Product
Last Things
Index
| Bibliographic details |
Paperback, 256 pages, publication date: FEB-2006
ISBN-13: 978-0-12-088545-9
ISBN-10: 0-12-088545-X
Imprint: ACADEMIC PRESS
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| Price and Ordering |
Price:
EUR 38.95
GBP 32.99
USD 54.95
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Last update: 5 Sep 2009
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