Edited by
Alan Kimmel, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health, Bethesda, Maryland, U.S.A.
Brian Oliver
Description
Modern DNA microarray technologies have evolved over the past 25 years to the point where it is now possible to take many million measurements
from a single experiment. These two volumes, Parts A & B in the
Methods in Enzymology series provide methods that will
shepard any molecular biologist through the process of planning, performing, and publishing microarray results.
Part A starts with
an overview of a number of microarray platforms, both commercial and academically produced and includes wet bench protocols for performing
traditional expression analysis and derivative techniques such as detection of transcription factor occupancy and chromatin status. Wet-bench
protocols and troubleshooting techniques continue into Part B. These techniques are well rooted in traditional molecular biology and
while they require traditional care, a researcher that can reproducibly generate beautiful Northern or Southern blots should have no
difficulty generating beautiful array hybridizations.
Data management is a more recent problem for most biologists. The bulk of Part
B provides a range of techniques for data handling. This includes critical issues, from normalization within and between arrays, to uploading
your results to the public repositories for array data, and how to integrate data from multiple sources. There are chapters in Part B
for both the debutant and the expert bioinformatician.
Included in series
Methods in Enzymology
Audience:
Biochemists and related researchers working with DNA microarray technologies.
