 |
|
| ENVIRONMENTAL MICROBIOLOGY
| |
| | |
|
|
A Laboratory Manual
To order this title, and for more information, click here
Second Edition
By
Ian Pepper, - University of Birmingham, B.Sc. in Chemistry, 1970 - Ohio State University, M.S. in Soil Biochemistry, 1972 - Ohio State University,
Ph.D. in SOil Microbiology, 1975, University of Arizona, Tucson, USA
Charles Gerba, - June 1969: Arizona State University, Tempe, Arizona, B.S., Microbiology - January 1973: University of Miami, Coral Gables, Florida, Ph.D. Microbiology, University of Arizona, Tucson, USA
Description
Environmental Microbiology: A Laboratory Manual is designed to meet the diverse requirements of upper division and graduate-level
laboratory sessions in environmental microbiology. The experiments introduce students to the activities of various organisms and the
analyses used to study them. The book is organized into three thematic sections: Soil Microbiology, Water Microbiology, and Environmental
Biotechnology. The first section includes experiments on the soil as a habitat for microorganisms, and introduces the main types of soil
microorganisms, how they interact with the soil, and the techniques used in their analysis. Experiments in the second section cover assays
of microbial pathogens--bacteria, viruses, and protozoan parasites--used in food and water quality control as well as an exercise in
applied bioremediation of contaminants in water. The final section on biotechnology includes applications of the polymerase chain reaction
(PCR) for the detection of bacteria and the use of enrichment cultures and a computer-based, physiological test bank to isolate and identify
a bacterium useful in bioremediation. Designed for maximum versatility and ease of use for both the student and instructor, each experiment
is self-contained and includes theoretical, practical, and pedagogical material.
Audience
Professional microbiologists working with soil and water; upper-division undergraduate and graduate students studying environmental microbiology.
Contents
Preface
Basic Protocols
Experiment 1 - Dilution and Plating of Bacteria and Growth Curves
Experiment
2 - Soil Moisture Content Determination
Examination of Soil Microorganisms via Microscopic and Cultural Assays
Experiment
3 - Contact Slide Assay
Experiment 4 - Filamentous Fungi
Experiment 5 - Bacteria and Actinomycetes
Experiment 6 - Algae: Enumeration
by MPN
Microbial Transformations and Response to Contaminants
Experiment 7 - Oxidation of Sulfur in SoilExperiment
8 - Dehydrogenase Activity of Soils
Experiment 9 - Nitrification and Denitrification
Experiment 10 - Enrichment and Isolation of Bacteria
that Degrade 2, 4-dichlorophenoxyacetic acid
Experiment 11 - Adaptation of Soil Bacteria to Metals
Experiment 12 - Biodegradation of
Phenol Compounds
Experiment 13 - Assimilable Organic Matter
Experiment 14 - Biochemical Oxygen Demand
Water Microbiology
Experiment 15 - Membrane Filter Technique
Experiment 16 - Bacteriological Examination of Water: the Coliform MPN Test
Experiment 17 -
Detection of Bacteriophages
Experiment 18 - Film Medium for the Detection of Coliforms in Water, Food, and on Surfaces
Experiment 19
- Defined Substrate Technology for the Detection of Coliforms and Fecal Coliforms
Advanced Topics
Experiment 20 - Detection
of Enteric Viruses in Water
Experiment 21 - Detection of Waterborne Parasites
Experiment 22 - Kinetics of Disinfection
Experiment 23
- Aerobiology: Sampling of Airborne Microorganisms
Experiment 24 - Detection and Identification of Bacteria via PCR and Subsequent BLAST
Analysis of Amplified Sequences
Appendix 1: Preparation of Media and Stains for Each Experiment
Appendix 2:
Glossary
| Bibliographic details |
Paperback, 232 pages, publication date: DEC-2004
ISBN-13: 978-0-12-550656-4
ISBN-10: 0-12-550656-2
Imprint: ACADEMIC PRESS
|
| Price and Ordering |
Price:
EUR 38.95
GBP 33
USD 48.95
|  |
Books and book related electronic products are priced in US dollars (USD), euro (EUR), and Great Britain Pounds (GBP). USD prices apply to the Americas and Asia Pacific. EUR prices apply in Europe and the Middle East. GBP prices apply to the UK and all other countries.
|
See also information about conditions of sale & ordering procedures, and links to our regional sales offices.
|
090/769
Last update: 5 Sep 2009
|
|
| |
| | |
|
|
| |
Home | Elsevier Sites | Privacy Policy | Terms and Conditions | Feedback | Site Map | A Reed Elsevier Company