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| CHEMOKINES, 287
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John Abelson, California Institute of Technology, Division of Biology, Pasadena, U.S.A.
Richard Horuk, Berlex Biosciences, Richmond, California, U.S.A.
Melvin Simon, California Institute of Technology, Division of Biology, Pasadena, USA
Description
Chemokines are major mediators of immune cells and are involved in a wide range of proinflammatory human diseases, including rheumatoid
arthritis, multiple sclerosis, and organ transplant rejection. It has recently been discovered that their receptors are involved in HIV
infection. The characterization of these molecules and their receptors is thus of primary importance in understanding a number of human
diseases and infections. This volume and its companion Volume 288 on Chemokine Receptors provide comprehensive
experimental protocols used in this field of research.
Audience
Biochemists, molecular biologists, immunologists, cell biologists, pharmacologists, and biomedical researchers.
Contents
C-X-C Chemokines:
R. Horuk, D. Reilly, and D. Yansura, Expression, Purification, and Characterization of Escherichia
coli Derived Recombinant Human Melanoma Growth Stimulating Activity.A. Wuyts, P. Proost,G. Froyen, A. Haelens, A. Billiau,
G. Opdenakker, and J. Van Damme, Purification and Identification of Human and Mouse Granulocyte Chemotactic Protein-2 Isoforms.
P.E. Dawson, Synthesis of Chemokines by Native Chemical Ligation.W.J. Fairbrother and H.B. Lowman, Molecular Approaches
to Structure-Function Analysis of Interleukin-8.J. Hesselgesser and R. Horuk, Alanine Scan Mutagenesis of Chemokines.D.
Baly, U. Gibson, D. Allison, and L. DeForge, Biological Assays for C-X-C Chemokines.K. Rajarathnam, C.M. Kay, I. Clark-Lewis,
and B.D. Sykes, Characterization of Quaternary Structure of Interleukin-8 and Functional Implications.
C-C and C Chemokines:
J. Van Damme, S. Struyf, A. Wuyts, G. Opdenakker, P. Proost, P. Allavena, S. Sozzani, and A. Mantovani, Isolation of Human Monocyte
Chemotactic Proteins and Study of their Producer and Responder Cells by Immunotests and Bioassays.A.-M. Buckle, S. Craig, and L.G.
Czaplewski, Assays for Macrophage Inflammatory Proteins.P.J. Nelson, J.M. Pattison, and A.M. Krensky, Gene Expression
of RANTES.A.M. Krensky and P.J. Nelson, Expression of Chemokine RANTES and Production of Monoclonal Antibodies.R.C. Newton
and K. Vaddi, Biological Responses to C-C Chemokines.D.N. Cook, Gene Targeting Strategies to Study Chemokine Function in Vivo.J.A. Hedrick and A. Zlotnik, Lymphotactin: A New Class of Chemokine.J.M. Schrider, Isolation and
Purification of Neutrophil Activating Peptide 4: A Chemokine Missing Two Cysteines.
Other Methods:
I. Clark-Lewis,
L. Vo, P. Owen, and J. Anderson, Chemical Synthesis Purification and Folding of C-X-C and C-C Chemokines.J.B. Smith and H.R.
Herschmann, Identification of Inflammatory Mediators by Screening for Glucocorticoid-Attenuated Response Genes.D.D. Taub, M.L.
Key, D.L. Longo, and W.J. Murphy, Chemokine-Induced Human Lymphocyte Infiltration and Engraftment in huPBL-SCID Mice.D.A. Barnes
and H.D. Perez, HighThroughput Screening for Identification of RANTES Chemokine Expression Inhibitors.S.A. Lira, M.E. Fuentes,
R.M. Strieter, and S.K. Durham, Transgenic Methods to Study Chemokine Function in Lung and Central Nervous System.R.M. Ransohoff,
M Tani, A.R. Glabinski, A. Chernosky, K. Krivacic, J.W. Peterson, H.-F. Chien, and B.D. Trapp, Chemokines and Chemokine Receptors
in Model Neurological Pathologies: Molecular and Immunocytochemical Approaches.R.E. Offord, H.F. Gaertner, T.N.C. Wells, andA.E.I.
Proudfoot, Synthesis and Evaluation of Fluorescent Chemokines Labeled at the Amino Terminal.G.S.V. Kuschert, R.E. Hubbard,
C.A. Power, T.N.C. Wells, and A.J. Hoogewerf, Solid-Phase Binding Assay to Study Interaction of Chemokines with Glycosaminoglycans.
M. Conklyn and H.J. Showell, Biological Activity of C-X-C and C-C Chemokines in Leukocyte Subpopulations in Human Whole Blood.
Author Index.
Subject Index.
| Bibliographic details |
Hardbound, 421 pages, publication date: SEP-1997
ISBN-13: 978-0-12-182188-3
ISBN-10: 0-12-182188-9
Imprint: ACADEMIC PRESS
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