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| HANDBOOK OF ENDOCRINE RESEARCH TECHNIQUES
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To order this title, and for more information, click here
Edited By
Flora de Pablo, Centro de Investigaciones Biologicas, C.S.I.C., Madrid, Spain
Colin Scanes
Bruce Weintraub
Description
Written by experts in the field, Handbook of Endocrine Research Techniques is currently the only single source of up-to-date methods and
strategies particularly useful in endocrinological research. As a resource for both the novice and experienced investigator, the book
includes chapters which provide an introduction to the area, general concepts, detailed protocols, and extensive references.
Contents
Hormone Assays. Radioimmunoassay (RIA): Concept and Types. Immunoradiometric (IRMA) Assays. Enzyme-Linked Immunoabsorbent Assays (ELISA).
Development and Characterization of Polyclonal and Monoclonal Antibodies. Isolation of Polypeptide Hormones. Reverse Hemolytic Plaque
Assays. Capillary Electrophoresis Coupled to Fluorescence Detection for the Determination of Multiple Neuropeptides. Immunoreactive Heterogeneity
in Peptide Measurement. Why Use a Flow Cytometer for Endocrine Cell Analysis? Identification and Characterization of Insulin-Like Growth
Factor Binding Proteins. Histochemical and in Situ Approaches. Localization of Peptides. Electron Microscopic Immunocytochemical Approaches
to the Localization of Ligands, Receptors, Transducers, And Transporters. In Situ Hybridization for Hormone and Receptor Expression.
Techniques for Receptors and Signal Transductors. Plasma Membrane Isolation Strategies for Cell Surface Receptors. Tyrosine Kinase Receptors.
Involvement of Protein Tyrosine Phosphatase Activity in the Regulation of Insulin's Signal Transduction. Techniques for Evaluating Protein
Kinase C in Intact Cells. Identification and Quantification of G Proteins. Determination of Adenylyl Cyclase Catalytic Activity. Intracellular
Mediators of Peptide Hormone Action. Free Calcium Measurements. Molecular Techniques and Specific Model Systems. Approaches for the Purification,
Quantitation, And Interpretation of Hormone and Receptor Mrnas. The Use of PCR in Endocrine Research. Detection of Mutations in Hormone
Receptor Genes. Techniques to Study Regulation of Gene Expression. Transgenic Animals. The Use of Non-Mammalian Models. Proliferation
Induced By Growth Factors in Mammalian Fetal Cells. Analysis of Hormones and Receptors in Embryogenesis.
| Bibliographic details |
Hardbound, 599 pages, publication date: OCT-1993
ISBN-13: 978-0-12-209920-5
ISBN-10: 0-12-209920-6
Imprint: ACADEMIC PRESS
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| Price and Ordering |
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Last update: 4 Sep 2009
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