To order this title, and for more information, click here Second Edition
Edited By Stefan Kaufmann, Max Planck Institute for Infection Biology, Berlin, Germany Dieter Kabelitz, University of Kiel, Germany
Description Immunology of Infection, 2nd Edition, edited by two leading experts in the field, presents the most appropriate up-to-date
experimental approaches in the detail required for modern microbiological research. Focusing on the methods most useful for the Microbiologist
interested in analysing host-pathogen relationships, this volume will be essential reading for all researchers working in microbiology,
immunology, virology, mycology and parasitology.
This new edition of Immunology of Infection provides ready-to-use
"recipes", and the latest emerging techniques as well as novel approaches to the tried and tested, established methods included in the
successful first edition.
Methods in Microbiologyis the most prestigious series devoted to techniques and methodology
in the field. Established for over 30 years, Methods in Microbiology will continue to provide you with tried and tested,
cutting edge protocols to directly benefit your research.
Audience
Microbiologists, immunologists, mycologists, and parasitologists
Contents Contributors
Preface to the 2nd edition
Preface to the 1st edition
Introduction
The immune response to infectious agents
General Methods
Phenotyping and separation of leukocyte populations based on affinity labelling.
Cytometric cytokine
secretion assay: detection and isolation of cytokine secreting T cells.
Measurement of cellular proliferation.
Use of bioinformatics
to predict MHC ligands and T-cell epitopes: application to epitope driven vaccine design.
The use of tetramers in the quantitative analysis
of T cell responses.
Using microarrays for studying the host transcriptional response to microbial infection.
Genome-wide expression
profiling of intracelllular bacteria: the interaction of Mycobacterium tuberculosis with macrophages.
Murine Models
Management of immuno-compromised and infected animals.In Vitro Analysis
Isolation and preparation of lymphocytes from infected
animals for in vitro analysis.
Isolation and development of murine T-lymphocyte hybridomas and clonal cell lines.
Killer cell assays.
Quantitation of T Cell Cytokine Responses by ELISA, ELISPOT, Flow Cytometry and Reverse Transcriptase-PCR Methods.
Isolation of and measuring
the function of professional phagocytes: murine macrophages.In vivo Analysis
The immune response in mice challenged with bacterial
infections.
Measuring immune responses in vivo.Specific Models
Murine model of tuberculosis.
The leishmaniasis model.
Animal
models: murine cytomegalovirus.
DNA vaccines: fundamentals and practice.
Preparation and use of adjuvants.
Human Systems
Isolation and propagation of human dendritic cells.
Isolation of T cells and establishment of T-cell lines and clones.
Growth-transformation
of human T cells.
Generation and characterisation of human killer cells.
Measuring human cytokine responses.
Measuring immune responses
in situ: immunofluorescent and immunoenzymatic techniques.
Isolation, characterisation and cultivation of human monocytes and macrophages.
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