To order this title, and for more information, click here Second Edition
Stefan Kaufmann, Max Planck Institute for Infection Biology, Berlin, Germany Dieter Kabelitz, University of Kiel, Germany
Description Immunology of Infection, 2nd Edition, edited by two leading experts in the field, presents the most appropriate up-to-date
experimental approaches in the detail required for modern microbiological research. Focusing on the methods most useful for the Microbiologist
interested in analysing host-pathogen relationships, this volume will be essential reading for all researchers working in microbiology,
immunology, virology, mycology and parasitology.
This new edition of Immunology of Infection provides ready-to-use
"recipes", and the latest emerging techniques as well as novel approaches to the tried and tested, established methods included in the
successful first edition.
Methods in Microbiologyis the most prestigious series devoted to techniques and methodology
in the field. Established for over 30 years, Methods in Microbiology will continue to provide you with tried and tested,
cutting edge protocols to directly benefit your research.
Audience
Microbiologists, immunologists, mycologists, and parasitologists
Contents Contributors
Preface to the 2nd edition
Preface to the 1st edition
Introduction
The immune response to infectious agents
General Methods
Phenotyping and separation of leukocyte populations based on affinity labelling
Cytometric cytokine
secretion assay: detection and isolation of cytokine secreting T cells
Measurement of cellular proliferation
Use of bioinformatics to
predict MHC ligands and T-cell epitopes: application to epitope driven vaccine design
The use of tetramers in the quantitative analysis
of T cell responses
Using microarrays for studying the host transcriptional response to microbial infection
Genome-wide expression profiling
of intracelllular bacteria: the interaction of Mycobacterium tuberculosis with macrophages
Murine Models
Management
of immuno-compromised and infected animalsIn Vitro Analysis
Isolation and preparation of lymphocytes from infected animals
for in vitro analysis
Isolation and development of murine T-lymphocyte hybridomas and clonal cell lines
Killer cell assays
Quantitation
of T Cell Cytokine Responses by ELISA, ELISPOT, Flow Cytometry and Reverse Transcriptase-PCR Methods
Isolation of and measuring the function
of professional phagocytes: murine macrophagesIn vivo Analysis
The immune response in mice challenged with bacterial infections
Measuring immune responses in vivoSpecific Models
Murine model of tuberculosis
The leishmaniasis model
Animal models: murine
cytomegalovirus
DNA vaccines: fundamentals and practice
Preparation and use of adjuvants
Human Systems
Isolation and
propagation of human dendritic cells
Isolation of T cells and establishment of T-cell lines and clones
Growth-transformation of human
T cells
Generation and characterisation of human killer cells
Measuring human cytokine responses
Measuring immune responses in situ:
immunofluorescent and immunoenzymatic techniques
Isolation, characterisation and cultivation of
human monocytes and macrophages
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