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| CHROMATIN, 304
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John Abelson, California Institute of Technology, Division of Biology, Pasadena, U.S.A.
Alan Wolffe, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland, U.S.A.
Melvin Simon, California Institute of Technology, Division of Biology, Pasadena, USA
Description
This volume of Methods in Enzymology includes up-to-date procedures used for the assembly of nucleosomes, chromatin,
and nuclei, extending the classical procedures described nearly ten years ago in Volume 170 (Nucleosomes) of this series,
and should assist in the further investigation of the ways in which the structural dynamics of chromatin contribute to geh regulation
of transcription, replication, recombination, and repair. Also described in this volume are assay for the structure and function of
in vitro reconstituted chromatin and for defining the organization and characteristics of natural chromosomal material from yeast
(Saccharomyces cerevisae), flies (Drosophila melanogaster), and frogs (Xenopus laevis), as well as from mammalian tissues. The purification
and assay procedures for various chromatin remodeling activities, including histone acetyltransferases, histone deacetylases, and SWI/SNF
ATPases are detailed.
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology
is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently
consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains
much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Audience
Graduate students and research scientists involved in biochemistry, molecular biology, cell biology, genetics, developmental biology, and biophysics.
Contents
Assembly of Nucleosomes, Chromatin, and Nuclei:
K. Luger, T.J. Rechsteiner, and T.J. Richmond, Preparation of
Nucleosome Core Particle from Recombinant Histones.L.M. Carrthers, C. Tse, K.P. Walker III, and J.C. Hansen, Assembly of Defined
Nucleosomal and Chromatin Arrays from Pure Components.J.A. Alfieri and D.J. Clark, Isolation of Minichromosomes from Yeast
Cells.D.J. Tremethick, Chromatin Assembly in Xenopus Extracts.A.J. Crowe and M.C. Barton, In Vitro Reconstitution
of Nucei for Replication and Transcription.L. Chang, C.A. Ryan, C.A. Schneider, and A.T. Annuziato, Preparation/Analysis of
Chromatin Replicated in Vivo and in Isolated Nuclei.M.E.A. Churchill, A. Changela, L.K. Dow, and A.J. Krieg, Interactions
of High Mobility Group Box Proteins with DNA and Chromatin. Y.V. Postnikov and M. Bustin, Reconstitution of High Mobility Group
14/17 Proteins into Nucleosomes and Chromatin.R. Reeves and M.S. Nissen, Purification and Assays for High Mobility Group I(Y)
Function.
Assays for Nucleosome Structure and Function in Vitro
.J. Bednar and C.L. Woodcock, Cryoelectron
Microscopic Analysis of Nucleosomes and Chromatin.J. Griffith, S. Michalowski, and A.M. Makhov, Electron Microscopy of DNA-Protein
Complexes and Chromatin.K.-M. Lee, D.R. Chafin, and J.F. Hayes, Targeted Cross-Linking and DNA Cleavage within Model Chromatin
Complexes.A. Flaus and T.J. Richmond, Base-Pair Resolution Mapping of Nucleosomes Positions Using Site-Directed Hydroxy Radicals.
S.I. Usachenko and E.M. Bradbury, Localization of Specific Histone Regions on Nucleosomal DNA by Cross-Linking.K.J. Polach
and J. Widom, Restriction Enzymes as Probes of Nucleosome Stability and Dynamics.S. Pennings, Nucleoprotein Gel Analysis
for Nucleosome Positioning and Mobility.Q. Li, U. Bjork and O. Wrange, Assays for Interaction of Transcription Factor with
Nucleosome.J.G. Moggs and G. Almouzni, Assays for Chromatin Remodeling During DNA Repair.K. Hirayoshi and J.T. Lis,
Nuclear Run-On Assays: Assessing Transcription by Measuring Density of Engaged RNA Polymerases.
Assays for Chromatin Structure
and Function in Vivo
.P.D. Gregory and W. Horz, Mapping Chromatin Structure in Yeast.M.P. Ryan, G.A.
Stafford, L. Yu, K.B. Cummings, and R.H. Morse, Assays for Nucleosome Positioning in Yeast.A. Hecht and M. Grunstein,
Mapping DNA Interaction Sites of Chromosomal Proteins Using Immunoprecipitation and PCR.P.B. Meluh and J.R. Broach, Immunological
Analysis of Yeast Chromatin. M.P. Kladde, M. Xu, and R.T. Simpson, DNA Methyltransferases as Probes of Chromatin Structure in Vivo. B. Suter, M. Livingstone-Zatchej, and F. Thoma, Mapping Cyclobutane-Pyrimidine Dimers in DNA and Using DNA
Repair by Photolyase for Chromatin Analysis in Yeast.I.L. Cartwright, D.E. Cryderman, D.S. Gilmour, L.A. Pile, L.L. Wallrath, J.A.
Weber, and S.C.R. Elgin, Analysis of Drosophila Chromatin Structure in Vivo.M.D. Biggin, Ultraviolet
Cross-Linking Assay to Measure Sequence-Specific DNA Binding in Vivo.D. Pruss, I.M. Gavin, S. Melnik, and S.G. Bavykin,
DNA-Protein Cross-Linking Applications for Chromatin Studies in Vitro and in Vivo.
C. Crane-Robinson, F.A. Myers,
T.R. Hebbes, A.L. Clayton, and A.W. Thorne, Chromatin Immunoprecipitation Assays in Acetylation Mapping of Higher Eukaryotes.G.P.
Pfeifer, H.H. Chen, J. Komura, and A.D. Riggs, Chromatin Structure Analysis by Ligation-Mediated and Terminal Transferase-Medicated
PCR.E.C. Strauss and S.H. Orkin, Guanine-Adenine Ligation-Mediated PCR in Vivo Footprinting.T.K. Archer and A.R.
Ricci, Exonuclease III as Probe of Chromatin Structure in Vivo.S. Belikov, O. Preobrazhenskaya, and V. Karpov,
Protein Image Hybridization: Mapping and Positioning DNA-Protein Contacts along DNA.K.S. Zaret, In Vivo Analysis of
Chromatin Structure.G.L. Hager and G. Fragoso, Analysis of Nucleosome Positioning in Mammalian Cells.P.R. Kramer, O. Bat,
and R.R. Sinden, Measurement of Localized DNA Supercoiling and Topological Domain Size in Eukaryotic Cells.W. Bickmore,
Fluorescent in Situ Hybridization Analysis of Chromosome and Chromatin Structure.M. Gotta, T. Laroche, S.M. Gasser,
Analysis of Saccharomyces cerevisiae Nuclear Organization.C.A. Mizzen, J.E. Brownell, R.G. Cook, and C.D. Allis, Histone
Acetyltransferases: Preparation of Substrates and Assay Procedures.N.A. Barlev, J.L. Workman, and S.L. Berger, Analysis of
Activity and Regulation of hGen5, Human Histone Acetyltransferase.P.A. Wade, P.L. Jones, D. Vermaak, and A.P. Wolffe, Purification
of Histone Deacetylase Complex from Xenopus laevis: Preparation of Substrates and Assay Procedures.C. Logie and C.L. Peterson,
Purification and Biochemical Properties of Yeast SWI/SNF Complex.P.D. Varga-Weisz, E.J. Bonte, and P.B. Becker, Analysis of
Modulators of Chromatin Structure in Drosophila.R. Sandaltzopoulos, V. Ossipow, D.A. Gdula, T. Tsukiyama, and C. Wu,
Purification of Drosophila Nucleosome Remodeling Factor.
| Bibliographic details |
Hardbound, 815 pages, publication date: MAY-1999
ISBN-13: 978-0-12-182205-7
ISBN-10: 0-12-182205-2
Imprint: ACADEMIC PRESS
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