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| SMALL GTPASES AND THEIR REGULATORS, PART A: RAS FAMILY, 255
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John Abelson, California Institute of Technology, Division of Biology, Pasadena, U.S.A.
W. Balch, The Scripps Research Institute, La Jolla, CA, USA
Melvin Simon, California Institute of Technology, Division of Biology, Pasadena, USA
Channing Der, Lineberger Comprehensive Cancer Center, University of North Carolina, Chapel Hill, USA
Alan Hall, University College of London, U.K.
Description
General Description of the Volume:
Small GTPases play a key role in many aspects of contemporary cell biology: control
of cell growth and differentiation; regulation of cell adhesion and cell movement; the organization of the actin cytoskeleton; and the
regulation of intracellular vesicular transport. This volume plus its companions Volumes 256 and 257 cover all the biochemical and biological
assays currently in use for analyzing the role of small GTPases in many aspects of cell biology at the molecular level.General
Description of the Series:
The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology
is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently
consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains
much material still relevant today--truly an essential publication for researchers in all fields of life sciences.
Audience
Biochemists, molecular biologists, cell biologists, pharmacologists, neuropsychologists, and neurochemists.
Contents
Expression, Purification, and Posttranslational Modification:
S.L. Campbell-Burk and J.W. Carpenter, Refolding
and Purification of Ras Proteins.E. Porfiri, T. Evans, G.E. Bollag, R. Clark, and J.F. Hancock, Purification of Baculovirus-Expressed
Recombinant Ras and Rap Proteins.G.E. Bollag and F. McCormick, Purification of Recombinant Ras GTPase-Activating Proteins.
B. Rubinfeld and P. Polakis, Purification of Baculovirus-Produced Rap 1 GTPase-Activating Proteins.G.L. James, M.S. Brown,
and J.L. Goldstein, Assays for Inhibitors for CAAX Farnesyltransferase in Vitro and in Intact Cells.R. Khosravi-Far
and C.J. Der, Prenylation Analysis of Bacterially Expressed and Insect Cell-Expressed Ras and Ras-Related Proteins.J.F. Hancock,
Reticulocyte Lysate Assay for in Vitro Translation and Posttranslational Modification of Ras Proteins.C. Volker and J.B.
Stock, Carboxyl Methylation of Ras-Related Proteins.F. Tamanoi and H. Mitsuzawa, Use of Yeast for Identification of Farnesyltransferase
Inhibitors and for Generation of Mutant Farnesyltransferases.
Guanine Nucleotide Exchange and Hydrolysis:
C. Lenzen,
R.H. Cool, and A. Wittinghofer, Analysis of Intrinsic and CDC25-Stimulated Guanine Nucleotide Exchange of p21ras--Nucleotide
Complexes by Fluorescence Measurements.J. Downward, Measurement of Nucleotide Exchange and Hydrolysis Activities in Immunoprecipitates.
J.B. Gibbs, Determination of Guanine Nucleotides Bound to Ras in Mammalian Cells.M. Frech, D. Cussac, P. Chardin, and D.
Bar-Sagi, Purification of Baculovirus-Expressed Human Sos1 Protein.M. Hart and S. Powers, Ras-Cdc25 and Rho-Dbl Binding
Assays: Complex Formation in Vitro.R.D. Mosteller, W. Park, and D. Broek, Analysis of Interaction between Ras and CDC25 Guanine Nucleotide Exchange Factor Using Yeast GAL4 Two-Hybrid System.T. Satoh and Y. Kaziro, Measurement
of Ras-Bound Guanine Nucleotide in Stimulated Hematopoietic Cells.A.M.M. de Vries-Smits, L. van der Voorn, J. Downward, and J.L.
Bos, Measurements of GTP/GDP Exchange in Permeabilized Fibroblasts.G.E. Bollag and F. McCormick, Intrinsic and GTPase-Activating
Protein-Stimulated Ras GTPase Assays.M.D. Schaber and J.B. Gibbs, Determination of Ras and GTPase-Activating Protein Interactions
by Kinetic Competition Assay.C. Ellis, V. Measday, and M.F. Moran, Phosphorylation-Dependent Complexes of p120 Ras-Specific
GTPase-Activating Proteins with p62 and p190.
Cell Expression and Analysis:
in Vitro
:A.D.
Cox, P.A. Solski, J.D. Jordan, and C.J. Der, Analysis of Ras Protein Expression in Mammalian Cells.A.M.M. deVries-Smits, B.M.T.
Burgering, and J.L. Bos, Vaccinia Virus Expression of p21rasAsn-17.H. Cai and G.M. Cooper, Inducible Expression
of Ras N17 Dominant Inhibitory Protein.J.F. Hancock, Prenylation and Palmitoylation Analysis.C.W.M. Reuter, A.D. Catling,
and M.J. Weber, Immune Complex Kinase Assays for Mitogen-Activated Protein Kinase and MEK.S. Kuroda, K. Shimizu, B. Yamamori,
and Y. Takai, Cell-Free Assay System for Ras-Dependent MEK Activation.P. Dent, G. Romero, D. Castle, and T.W. Sturgill,
Preparation and Use of Semiintact Mammalian Cells for Analysis of Signal Transduction.C.J. Marshall and S.J. Leevers, Mitogen-Activated
Protein Kinase Activation by Scrape Loading of P21ras.D.R. Alessi, P. Cohen, A. Ashworth, S. Cowley, S.J. Leevers, and C.J. Marshall,
Assay and Expression of Mitogen-Activated Protein Kinase, MAP Kinase Kinase, and Raf.C.A. Lange-Carter and G.L. Johnson, Assay
of MEK Kinases.D.K. Morrison, Activation of Raf-1by Ras in Intact Cells.R. Finney and D. Herrera, Ras-Raf Complexes:
Analyses of Complexes Formed in Vivo.X.-f. Zhang, M.S. Marshall, and J. Avruch, Ras-Raf Complexes in Vitro.
A.B. Vojtek and S.M. Hollenberg, Ras-Raf Interaction: Two-Hybrid Analysis.J.K. Westwick and D.A. Brenner, Methods
for Analyzing c-Jun Kinase.B. Margolis, E.Y. Skolnik, and J. Schlessinger, Use of Tyrosine-Phosphorylated Proteins to Screen
Bacterial Expression Libraries for SH2 Domains.S.M. Feller, B. Knudsen, T.W. Wong, and H. Hanafusa, Detection of SH3-Binding
Proteins in Total Cell Lysates with Glutathine S-Transferase-SH3 Fusion Proteins: SH3 Blot Assay.N.E. Kohl, F.R. Wilson,
T.J. Thomas, R.L. Bock, S.D. Mosser, A. Oliff, and J.B. Gibbs, Inhibition of Ras Function in Vitro and in Vivo
Using Inhibitors of Farnesyl-Protein Transferase.
Biological Activity:
C. Finlay, Rat Embryo Fibroblast Complementation
Assay with ras Genes.G.J. Clark, A.D. Cox, S.M. Graham, and C.J. Der, Biological Assays for Ras Transformation.C.A.
Hauser, J.K. Westwick, and L.A. Quilliam, Ras-Mediated Transcription Activation: Analysis by Transient Cotransfection Assays.B.
Tocque, M. Janicot, and M. Kenigsberg, Oocyte Microinjection Assay for Evaluation of Ras-Induced Signaling Pathways.D. Bar-Sagi,
Mammalian Cell Microinjection Assays.A. Mikheev, R.S. Cha, and H. Zarbl, Detection of Point Mutations in Ras in Tumor Cell
Lines by Denaturant Gradient Gel Electrophoresis.S.M. Kahn, W. Jiang, I.B. Weinstein, and M. Perucho, Diagnostic Detection
of Mutant ras Genes in Minor Cell Populations.L. Chen and S. Powers, Ras in Yeast: Complementation Assays for Testof
Function.K.A. Mintzer and J. Field, Yeast Adenylyl Cyclase Assays.M.T. Quinn, C.A. Parkos, and A.J. Jesaitis, Purification
of Human Neutrophil NADPH Oxidase Cytochrome b-588 and Association with Rap1A.P. Poullet and F. Tamanoi, Use of Yeast
Two-Hybrid System to Evaluate Ras Interactions with Neurofibromin-GTPase-Activating Protein.A.B. Sparks, N.B. Adey, L.A. Quilliam,
J.M. Thorn, and B.K. Kay, Screening Phage-Displayed Random Peptide Libraries for SH3 Ligands.
Author Index.
Subject Index.
| Bibliographic details |
Hardbound, 548 pages, publication date: AUG-1995
ISBN-13: 978-0-12-182156-2
ISBN-10: 0-12-182156-0
Imprint: ACADEMIC PRESS
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